Urinary Hydatid Antigen Detection by Coagglutination, a Cost-Effective and Rapid Test for Diagnosis of Cystic Echinococcosis in a Rural or Field Setting

Ravinder, Prajwal; Parija, Subhash Chandra; Rao, K. S. V. K. Subba

doi:10.1128/jcm.38.8.2972-2974.2000

Cited by 16 publications

(5 citation statements)

References 13 publications

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“…Therefore, there is a need for rapid diagnostic tools to prevent high economic losses. A coagglutination kit can be considered as one such test [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

et al. 2020

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Background and Aim: Newcastle disease (ND) is a viral infection that causes high mortality and economic loss in the poultry industry. The Office International des Epizooties (OIE) recommends several diagnostic methods for the detection of ND, including isolation and molecular tests. However, these detection methods are time-consuming and highly expensive. Therefore, this study was conducted to develop a coagglutination kit as a novel diagnostic tool for ND in the poultry industry. Materials and Methods: Two adult male New Zealand White rabbits weighing 2.5 kg were vaccinated using ND life vaccine intraperitoneally. The vaccination was conducted once a week for 4 weeks with multilevel doses. Rabbits' serum was collected at week 6 and inactivated at 56°C for 30 min. The serum was precipitated using ammonium sulfate and reacted with protein A of Staphylococcus aureus to produce the agglutination kit for detecting ND virus. A total of 25 chickens suspected with ND infection from a local poultry farm in Yogyakarta were used as the test samples. The chickens were necropsied, and the brain, spleen, lung, intestine, and feces were collected. Half of these organs were subjected to tests using the coagglutination kit and reverse transcription-polymerase chain reaction (RT-PCR). The other half was processed for histopathology. Data were analyzed qualitatively. Results: Of the 25 samples, 13 (52%) were positive for ND infection when tested using both the ND coagglutination kit and RT-PCR. The positive samples also exhibited several histopathological changes, including perivascular cuffing surrounding the cerebral blood-brain barrier, hemorrhagic pneumonia, splenitis, and necrotic hemorrhage enteritis. Conclusion: This study confirmed that the ND coagglutination kit could be used as a novel diagnostic tool for the detection of ND virus infection in the poultry industry.

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“…Therefore, there is a need for rapid diagnostic tools to prevent high economic losses. A coagglutination kit can be considered as one such test [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

et al. 2020

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“…Urinary hydatid antigen detection by co-agglutination (Co-A) potentially represents a costeffective and rapid test for diagnosis of CE in a rural or field setting. However, the lower sensitivity of Co-A for detection of antigen in the urine of a patient whose serum was positive for the antigen is possibly due to low levels of antigen in the urine (Ravinder et al, 2000).…”

Section: Circulating Antigen Detectionmentioning

confidence: 99%

Serological Diagnosis of Certain Human, Animal and Plant Diseases

Al-Moslih¹

2012

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“…The circulating antigen would also normally disappear quite rapidly upon successful treatment. ELISA , latex agglutination test , immune‐electro‐transfer blot , and co‐agglutination test were previously used for detection of CE antigen in urine, serum, and saliva. However, these methods showed low sensitivity and relatively high cost and require trained personnel.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

2020

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Cystic echinococcosis (CE) or hydatid disease is a parasitic infection caused by Echinococcus granulosus. Early serodiagnosis and continuous monitoring of the disease is very important for medical treatment. Here, we report the detecting of both echinococcus antigen and antibody for the diagnosis of hydatid disease using square wave voltammetry (SWV)‐based immunosensors. The gold electrodes were functionalized using cysteamine/phenylene diisothiocyanate linkers and used for the immunosensors fabrication. The hydatid antigen and antibody immunosensors were constructed by the immobilization of either purified rabbit polyclonal antibody or recombinant antigen B (AgB), respectively on the functionalized gold electrodes surfaces. The detection in both cases was achieved by following the change in the SWV reduction peak current of the ferro/ferricyanide redox couple upon antibody or antigen binding. These immunosensors enabled the detection of echinococcus antigen and antibody within a concentration range of 1 pg.mL−1 to 1 μg.mL−1 with detection limits of 0.4 pg.mL−1 and 0.3 pg.mL−1, respectively. A preliminary application of the developed immunosensor was performed in spiked serum sample showing good recovery percentages ranging from 102 to 110 % for both hydatid antibody and antigen detection. This easy‐to‐use, sensitive, and low cost quantitative method holds great promise for the early diagnosis of hydatid disease and thus, better managements and treatment outcomes.

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Urinary Hydatid Antigen Detection by Coagglutination, a Cost-Effective and Rapid Test for Diagnosis of Cystic Echinococcosis in a Rural or Field Setting

Cited by 16 publications

References 13 publications

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

Serological Diagnosis of Certain Human, Animal and Plant Diseases

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

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