Urinary microbiome profile in men with genitourinary malignancies

Ahn, Hyun Kyu; Kim, Kwangmin; Park, Jun Hyung; Kim, Kwang Hyun

doi:10.4111/icu.20220124

Cited by 20 publications

(7 citation statements)

References 30 publications

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“…The bacteria discovered in this study, such as Sphingomonas , Propionibacterium , and Corynebacterium , have been reported in several previous studies on urinary microbiota ( Ahn et al, 2022 ; Cappelli et al, 2023 ; Nickel et al, 2022 ; Perovic et al, 2022 ; Popovic et al, 2018 ; Kim & Park, 2018 ). However, Methylophilus , identified in this study, has only been reported in earlier studies on human skin and gut microbiota ( Dekio et al, 2005 ; Jiang et al, 2021b ; Jiang et al, 2021b ; Lee et al, 2021 ; Zheng et al, 2023 ).…”

Section: Discussionsupporting

confidence: 69%

Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients

Li,

Tang,

et al. 2024

PeerJ

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Objectives Type 2 diabetes mellitus (T2DM) commonly complicates kidney stone disease (KSD). Our objective is to investigate the variations in the urinary microbiota between individuals with KSD alone and those with KSD plus T2DM. This exploration could have implications for disease diagnosis and treatment strategies. Methods During lithotripsy, a ureterscope was employed, and 1 mL of urine was collected from the renal pelvis after bladder disinfection. Sequencing targeting the V3–V4 hypervariable region was performed using the 16S rRNA and Illumina Novaseq platform. Results The Shannon index showed a significant decrease in the KSD plus T2DM group compared to the KSD-only group (false discovery rate = 0.041). Principal Coordinate Analysis (PCoA) demonstrated a distinct bacterial community in the KSD plus T2DM group compared to the KSD-only group (false discovery rate = 0.027). The abundance of Sphingomonas, Corynebacterium, and Lactobacillus was significantly higher in the KSD plus T2DM group than in the KSD-only group (false discovery rate < 0.05). Furthermore, Enhydrobacter, Chryseobacterium, and Allobaculum were positively correlated with fasting blood glucose and HbA1c values (P < 0.05). Conclusions The urinary microbiota in the renal pelvis exhibits differences between patients with KSD plus T2DM and those with KSD alone. Further studies employing animal models are necessary to validate these distinctions, potentially paving the way for therapeutic developments based on the urinary microbiota.

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Section: Discussionsupporting

confidence: 69%

Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients

Li,

Tang,

et al. 2024

PeerJ

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“…(2023) Sphingomonas is widely present in the environment and has an environmental restorative role ( Asaf et al., 2020 ). Hyun Kyu Ahn et al. (2022) revealed that, compared with other urological tumors, there was a higher content of Sphingomonas in BCa compared to other urological tumors.…”

Section: Discussionmentioning

confidence: 94%

The bladder microbiome of NMIBC and MIBC patients revealed by 2bRAD-M

Sun

Xia

Zhong

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundBladder cancer (BCa) is the most common malignancy of the urinary tract which can be divided into non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC), and their microbial differences are not fully understood. This study was conducted by performing 2bRAD sequencing for Microbiome (2bRAD-M) on NMIBC and MIBC tissue samples to investigate the microbiota differences between NMIBC and MIBC individuals.MethodsA total of 22 patients with BCa, including 7 NMIBC and 15 MIBC, were recruited. Tumor tissues were surgically removed as samples and DNA was extracted. Type IIB restriction endonucleases were used to enzymatically cleave the microbial genome for each microbe’s tag and map it to species-specific 2bRAD markers to enable qualitative and quantitative studies of microbes between MIBC and NMIBC tissues.ResultsA total of 527 species were detected. The microbial diversity of NMIBC tissues was significantly higher than that of MIBC tissues. Microbial composition of the two tumor tissues was similar, where Ralstonia_sp000620465 was the most dominant species. 4 species (Acinetobacter_guillouiae, Anoxybacillus_A_rupiensis, Brevibacillus_agri and Staphylococcus_lugdunensis) were enriched in NMIBC, while Ralstonia_mannitolilytica, Ralstonia_pickettii, and Ralstonia_sp000620465 were overrepresented in MIBC. 252 discriminatory character taxa were also revealed by linear discriminant analysis effect sizea (LEfSe). Species importance point plots identified Ralstonia_sp000620465, Cutibacterium_acnes and Ralstonia_pickettii as the three most important species between the two groups. Meanwhile, functional annotation analysis showed 3011 different COGs and 344 related signaling pathways between MIBC and NMIBC microbiome.ConclusionThis first 2bRAD-M microbiome study on MIBC and NMIBC tissues revealed significant differences in the microbial environment between the two groups, which implies a potential association between tumor microbial dysbiosis and BCa, and provides a possible target and basis for subsequent studies on the mechanisms of BCa development and progression.

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“…Hyun Kyu Ahn et al, demonstrated that there is no difference in alpha- and beta-diversity between renal cell carcinoma patients and healthy individuals. They also identified 8 species, the abundance of which differs significantly in renal cell carcinoma patients, with 6 of these— C. acnes , P. lacydonensis , Micrococcus spp ., C. granulosum , Tessaracoccus arenae , and Staphylococcus epidermidis were more abundant in the renal cell carcinoma group [ 24 ].…”

Section: Urinary Microbiomementioning

confidence: 99%

Resident Microbiome of Kidney Tumors

Kovaleva,

Podlesnaya,

Gratchev

2024

Oncol.Rev.

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Emerging research has uncovered the significance of microbiota in carcinogenesis, with specific bacterial infectious agents linked to around 15% of malignant tumors. This review is focused on the resident kidney microbiome, its composition, and alterations in various diseases. Recent studies have shown that bacteria can infiltrate the kidney, with differences between normal and tumor tissue. These studies have identified distinctive microorganisms unique to both conditions, hinting at their potential clinical relevance. Research into the kidney microbiome diversity reveals differences in tumor tissue, with specific taxa associated with different histological types. Notably, the alpha diversity indices suggest variations in bacterial content between tumor and normal tissue, offering insights into potential diagnostic and prognostic use of these markers. Better studied is the impact of the gut microbiome on therapy efficacy in malignant kidney tumors. Antibiotics, which can alter the gut microbiome, have been linked to survival outcomes in patients receiving targeted therapy and immunotherapy. The findings suggest that the uncontrolled use of antibiotics may not only contribute to bacterial resistance but also disrupt the normal microbiome, potentially influencing the development of oncological diseases. In-depth investigation into the resident kidney microbiome is essential for addressing fundamental and practical aspects of kidney tumor development.

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Urinary microbiome profile in men with genitourinary malignancies

Cited by 20 publications

References 30 publications

Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients

Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients

The bladder microbiome of NMIBC and MIBC patients revealed by 2bRAD-M

Resident Microbiome of Kidney Tumors

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