Urinary NGAL, cystatin C, β2-microglobulin, and osteopontin significance in hydronephrotic children

Madsen, Mia Gebauer; Nørregaard, Rikke; Palmfeldt, Johan; Olsen, Lars Henning; Frøkiær, Jørgen; Jørgensen, Troels Munch

doi:10.1007/s00467-012-2217-6

Cited by 59 publications

(64 citation statements)

References 23 publications

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Section: Discussionmentioning

confidence: 99%

“…Changes in the urinary excretion of B2M, cystatin C, osteopontin, and uromodulin have been associated with varying forms of kidney injury, 27,33,[50][51][52][53][54][55]231 but were not different between preeclampsia cases and controls. Increased urine NGAL has been associated with acute kidney injury, 51,56,57 but we found levels to be decreased in preeclampsia. EGF has been shown to be decreased in acute renal failure, obstructive nephropathy, and diabetic nephropathy, 29,58,59 and we also found decreased urinary levels in preeclampsia.…”

Section: Discussionmentioning

confidence: 99%

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Complement Activation and Kidney Injury Molecule-1–Associated Proximal Tubule Injury in Severe Preeclampsia

et al. 2014

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We enrolled 25 cases with severe preeclampsia, 25 controls with chronic hypertension, and 25 healthy controls without hypertension from a cohort of women receiving care at Brigham and Women's Hospital from March 2012 through March 2013. Institutional review board approval was obtained through the Partners Human Research Committee, and subjects gave informed consent. All procedures followed were in accordance with institutional guidelines. Methods Abstract-Kidney injury with proteinuria is a characteristic feature of preeclampsia, yet the nature of injury in specific regions of the nephron is incompletely understood. Our study aimed to use existing urinary biomarkers to describe the pattern of kidney injury and proteinuria in pregnancies affected by severe preeclampsia. We performed a case-control study of pregnant women from Brigham and Women's Hospital from 2012 to 2013. We matched cases of severe preeclampsia (n=25) 1:1 by parity and gestational age to 2 control groups with and without chronic hypertension. Urinary levels of kidney injury molecule-1 and complement components (C3a, C5a, and C5b-9) were measured by enzyme-linked immunosorbent assay, and other markers (albumin, β2 microglobulin, cystatin C, epithelial growth factor, neutrophil gelatinase-associated lipocalin, osteopontin, and uromodulin) were measured simultaneously with a multiplex electrochemiluminescence assay. Median values between groups were compared with the Wilcoxon signed-rank test and correlations with Spearman correlation coefficient. Analysis of urinary markers revealed higher excretion of albumin and kidney injury molecule-1 and lower excretion of neutrophil gelatinase-associated lipocalin and epithelial growth factor in severe preeclampsia compared with chronic hypertension and healthy controls. Among subjects with severe preeclampsia, urinary excretion of complement activation products correlated most closely with kidney injury molecule-1, a specific marker of proximal tubule injury (C5a: r=0.60; P=0.001; and C5b-9: r=0.75; P<0.0001). Taken together, we describe a pattern of kidney injury in severe preeclampsia that is characterized by glomerular impairment and complement-mediated inflammation and injury, possibly localized to the proximal tubule in

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Complement Activation and Kidney Injury Molecule-1–Associated Proximal Tubule Injury in Severe Preeclampsia

et al. 2014

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“…Also the concentration of RBP4 in serum is the major indicator of the newborn's birth weight [35]. The level of β2-MG (β2-microglobulin) can also be used for evaluating the function of the renal tubules of the kidneys [39], in particular, in term babies with perinatal asphyxia [40] and children with pneumonia [41].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Investigation of urine proteome of preterm newborns with respiratory pathologies

Стародубцева

Kononikhin

Бугрова

et al. 2016

Journal of Proteomics

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“…7-9 In the setting of UPJO a recent observational study described increased NGAL in the renal pelvic urinary aspirates of patients undergoing pyeloplasty compared to controls. 10 However, these data were not correlated with any clinical end points.…”

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confidence: 92%

Urinary NGAL Levels Correlate with Differential Renal Function in Patients with Ureteropelvic Junction Obstruction Undergoing Pyeloplasty

et al. 2013

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Purpose: Recent investigations described the use of NGAL, a sensitive biomarker for kidney injury, in the setting of ureteropelvic junction obstruction. We prospectively evaluated urinary NGAL levels in the affected renal pelvis and bladder of children with ureteropelvic junction obstruction undergoing unilateral dismembered pyeloplasty. Our hypothesis was that higher NGAL in the kidney and bladder would correlate with decreased ipsilateral differential function. Materials and Methods: We performed a prospective cohort study in patients treated with unilateral dismembered pyeloplasty from 2010 to 2012. Urine was obtained intraoperatively from the bladder and obstructed renal pelvis. A control population of unaffected children was recruited to provide a voided bladder specimen. Bladder NGAL levels were compared between the study and control populations. We tested our study hypothesis by correlating bladder and renal pelvic NGAL levels with the differential renal function of the affected kidney. Results: A total of 61 patients with a median age at surgery of 1.62 years (range 0.12 to 18.7) were enrolled in the study. Median bladder NGAL was 18.6 ng/mg (range 1.4-1,650.8) and median renal pelvic NGAL was 26.2 ng/mg (range 1.2-18,034.5, p = 0.004). Median bladder NGAL was significantly higher than in controls (p = 0.004). The correlation of bladder and renal pelvic NGAL with differential renal function was r = −0.359 (p = 0.004) and r = −0.383 (p = 0.002), respectively. Conclusions: Bladder NGAL is increased in children with ureteropelvic junction obstruction. Renal pelvic and bladder normalized urinary NGAL levels correlate inversely with the relative function of the affected kidney in cases of unilateral ureteropelvic junction obstruction.

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Urinary NGAL, cystatin C, β2-microglobulin, and osteopontin significance in hydronephrotic children

Abstract: This study confirms increased concentrations of NGAL and β2-M in urine from obstructed kidneys and should be tested in larger studies to ascertain their ability to identify obstruction and to determine the importance of age-adjusted reference values.

Cited by 59 publications

References 23 publications

Complement Activation and Kidney Injury Molecule-1–Associated Proximal Tubule Injury in Severe Preeclampsia

Complement Activation and Kidney Injury Molecule-1–Associated Proximal Tubule Injury in Severe Preeclampsia

Investigation of urine proteome of preterm newborns with respiratory pathologies

Urinary NGAL Levels Correlate with Differential Renal Function in Patients with Ureteropelvic Junction Obstruction Undergoing Pyeloplasty

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