Urine nitrification with a synthetic microbial community

Christiaens, Marlies; Paepe, Jolien De; Ilgrande, Chiara; Vrieze, Jo De; Barys, Justyna; Teirlinck, Pieter; Meerbergen, Ken; Lievens, Bart; Boon, Nico; Clauwaert, Peter; Vlaeminck, Siegfried E.

doi:10.1016/j.syapm.2019.126021

Cited by 14 publications

(19 citation statements)

References 43 publications

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“…According to this analysis and in light of the fact that they are often used interchangeably, we conclude that the terms microbial consortium, synthetic microbial community, microbial cocktail, and synthetic microbiome Del Frari et al, 2019;El Hage et al, 2019;Colpa et al, 2020;Pereira et al, 2020;Dai et al, 2021 Chen et al, 2018;Wang et al, 2018;Rafique et al, 2019;Rékási et al, 2019;Dong et al, 2020;Singh et al, 2020 Synthetic microbial community Shahab et al, 2018;Carrión et al, 2019;Christiaens et al, 2019;Honjo et al, 2019;Wang et al, 2020;Krieger et al, 2021 Microbial cocktail Moreira et al, 2017;Qu et al, 2018;Pasaribu et al, 2019;Pires et al, 2019;Tobin et al, 2020;Zheng et al, 2020 Synthetic microbiome Venturelli et al, 2018;Timmis et al, 2019;Vandeplassche et al, 2019;Voges et al, 2019;Koskella, 2020;Mabwi et al, 2021 The symbol "+" indicates the presence of the specific key characteristic, while the symbol "-" indicates its absence, in association with each term (refer to the text for the definition of the concepts of each key characteristic).…”

Section: Are Microbial Blend-related Terms Synonyms?mentioning

confidence: 80%

“…The numerous advantages of using microbial blends over microbiomes or single strains have been attracting a growing interest in several fields of science (Compant et al, 2019;Santos et al, 2019). Among the numerous applications of microbial blends, we find probiotics (El Hage et al, 2019), biotechnology (Christiaens et al, 2019), bioremediation (Brune and Bayer, 2012), biocontrol (Del Frari et al, 2019), ecology (Voges et al, 2019), human disease (Pereira et al, 2020), crop enhancement (Allaga et al, 2020), and more. In addition, recent advances in microbial blend engineering (Lawson et al, 2019) suggest that this technology will strongly contribute to re-shape microbiological research in the near future.…”

Section: Microbiomes and Microbial Blendsmentioning

confidence: 99%

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Microbial Blends: Terminology Overview and Introduction of the Neologism “Skopobiota”

Frari

Ferreira

2021

Front. Microbiol.

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Section: Are Microbial Blend-related Terms Synonyms?mentioning

confidence: 80%

Section: Microbiomes and Microbial Blendsmentioning

confidence: 99%

Microbial Blends: Terminology Overview and Introduction of the Neologism “Skopobiota”

Frari

Ferreira

2021

Front. Microbiol.

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“…This method bypasses the separate aerobic digestion step where nitrification may normally be present. Though previous stabilization steps still apply, Christiaens et al (2019) were able to achieve N production rates of 29 mg nitrate L −1 d −1 in 10% diluted urine even under high sodium pressures. Udert and Wächter (2012) developed a system combining distillation with nitrification to completely recover nutrients from urine.…”

Section: Nitrificationmentioning

confidence: 99%

Optimizing Nitrogen Fixation and Recycling for Food Production in Regenerative Life Support Systems

Langenfeld

Kusuma

Wallentine

et al. 2021

Front. Astron. Space Sci.

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Nitrogen (N) recycling is essential for efficient food production in regenerative life support systems. Crew members with a high workload need 90–100 g of protein per person per day, which is about 14 g of N, or 1 mole of N, per person per day. Most of this N is excreted through urine with 85% as urea. Plants take up N predominantly as nitrate and ammonium, but direct uptake as urea is possible in small amounts. Efficient N recycling requires maintenance of pH of waste streams below about 7 to minimize the volatilization of N to ammonia. In aerobic reactors, continuous aerobic conditions are needed to minimize production and volatilization of nitrous oxide. N is not well recycled on Earth. The energy intensive Haber–Bosh process supplies most of the N for crop production in terrestrial agriculture. Bacterial fixation of dinitrogen to ammonium is also energy intensive. Recycling of N from plant and human waste streams is necessary to minimize the need for N fixation. Here we review approaches and potential for N fixation and recycling in regenerative life support systems. Initial estimates indicate that nearly all the N from human and plant waste streams can be recovered in forms usable for plants.

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“…For nitrification of ammonium to nitrate, the MELiSSA system uses a synthetic community of two axenic autotrophic strains, i.e., Nitrosomonas europaea ATCC 19718 and Nitrobacter winogradskyi ATCC 25391, instead of a mixed undefined community from wastewater sludge ( Cruvellier et al, 2016 ). An axenic co-culture does not perform significantly better than its open culture counterparts in a bioreactor ( Zeghal et al, 1994 ), but it is easier to study, characterize, and model ( Christiaens et al, 2019a ). N. winogradskyi is the NOB considered in the MELISSA CIII because out of all NOB strain candidates studied, it proved to be the least affected by high concentrations of both ammonium and nitrite.…”

Section: Nitrogen Recovery In CIII Of Melissamentioning

confidence: 99%

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

et al. 2021

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To enable long-distance space travel, the development of a highly efficient and robust system to recover nutrients from waste streams is imperative. The inability of the current physicochemical-based environmental control and life support system (ECLSS) on the ISS to produce food in situ and to recover water and oxygen at high enough efficiencies results in the need for frequent resupply missions from Earth. Therefore, alternative strategies like biologically-based technologies called bioregenerative life support systems (BLSSs) are in development. These systems aim to combine biological and physicochemical processes, which enable in situ water, oxygen, and food production (through the highly efficient recovery of minerals from waste streams). Hence, minimalizing the need for external consumables. One of the BLSS initiatives is the European Space Agency’s (ESA) Micro-Ecological Life Support System Alternative (MELiSSA). It has been designed as a five-compartment bioengineered system able to produce fresh food and oxygen and to recycle water. As such, it could sustain the needs of a human crew for long-term space exploration missions. A prerequisite for the self-sufficient nature of MELiSSA is the highly efficient recovery of valuable minerals from waste streams. The produced nutrients can be used as a fertilizer for food production. In this review, we discuss the need to shift from the ECLSS to a BLSS, provide a summary of past and current BLSS programs and their unique approaches to nitrogen recovery and processing of urine waste streams. In addition, compartment III of the MELiSSA loop, which is responsible for nitrogen recovery, is reviewed in-depth. Finally, past, current, and future related ground and space demonstration and the space-related challenges for this technology are considered.

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Urine nitrification with a synthetic microbial community

Cited by 14 publications

References 43 publications

Microbial Blends: Terminology Overview and Introduction of the Neologism “Skopobiota”

Microbial Blends: Terminology Overview and Introduction of the Neologism “Skopobiota”

Optimizing Nitrogen Fixation and Recycling for Food Production in Regenerative Life Support Systems

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

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