Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy

Haubitz, Marion; Wittke, Stefan; Weissinger, Eva M.; Walden, Michael; Rupprecht, Harald; Floege, J.; Haller, Hermann; Mischak, Harald

doi:10.1111/j.1523-1755.2005.00335.x

Cited by 211 publications

(183 citation statements)

References 17 publications

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“…These patterns can be used for diagnostic purposes, since each renal disease produces a specific and typical urinary polypeptide pattern. The discrimination between different primary renal diseases was high with a sensitivity and specificity above 90% [41,54]. These results indicate that a "common biomarker", which should display unique characteristics (presence/absence or signal intensity) in each sample from patients with a defined kidney disease does not exist.…”

Section: Biomarker Discovery In Urinementioning

confidence: 75%

“…As evident from recent reports, this appears to be correct [12,41,[54][55][56], but a serious obstacle to this venture is the variability of polypeptides examined. Using CE-MS we documented that polypeptides in urine change significantly during the day, most likely as a consequence of physical activity, diet, smoking, etc.…”

Section: Biomarker Discovery In Urinementioning

confidence: 93%

“…To this end, we analyzed more than 100 samples from healthy subjects and identified a "normal" urine polypeptide pattern [12,40,41]. Moreover, using CE-MS together with the MosaiquesVisu software, we established characteristic polypeptide patterns for patients with various renal diseases, e.g., minimal-change disease, focalsegmental glomerulosclerosis, membranous glomerulonephritis, Immunoglobulin (IgA) nephropathy, and diabetic nephropathy [12,41,54,55]. These patterns can be used for diagnostic purposes, since each renal disease produces a specific and typical urinary polypeptide pattern.…”

Section: Biomarker Discovery In Urinementioning

confidence: 99%

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Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes

2005

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Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposesThe introduction of fast, sensitive, and robust techniques for proteomic analysis into clinical practice represents a major step toward a new diagnostic approach of body fluids. In addition, proteomics emerges as a key technology for the discovery of disease biomarkers in various body fluids. However, even in relatively protein-deprived body fluids such as urine, the complexity and wide dynamic range of protein expression pose a considerable challenge to both separation and identification technologies. In the present review we discuss from a clinical point-of-view recent advances of the use of proteomics in clinical diagnosis as well as therapy evaluation. We focus on capillary electrophoresis coupled to mass spectrometry (CE-MS) and discuss CE-MS from an application point of view evaluating its merits and vices with regard to biomarker discovery. This review further presents examples of clinical applications of CE-MS for detection and identification of biomarkers in urine. IntroductionIt is well appreciated for centuries that body fluids contain information on the "internal" environment, i.e., the function of tissues and organs, which can be used as a gauge for the well being of the organism. Although this concept was created by Hippocrates more than 2000 years ago, standardized clinical laboratory tests were not developed before the 20th century. Moreover, a systematic discovery of biomarkers was made possible only with the discovery of antibodies and their use in immunological assays such as radioimmuno assay (RIA), ELISA, etc. Their increasing use enabled detection and quantification of distinct molecules -mainly peptides and proteins -in various body fluids. However, these techniques were limited by their requirement of prior knowledge of the molecule and a specific probe. New functional proteomic approaches appeared to circumvent these limitations. Spurred by advances in MS it was hoped that proteomics would allow the analysis of thousands of peptides and proteins in one sample [1][2][3][4][5][6], but several obstacles turned up soon. The high complexity and wide dynamic range of peptides in body fluids required pre-MS separation techniques that offer high resolution and could cope with a wide dynamic range of peptide concentrations [3]. Further, suitable software for interpretation of a vast number of generated MS spectra as well as comparison of the data was a major challenge [7].The approach towards solving most of these problems was to reduce data complexity, for example by arbitrarily collecting data on only a few polypeptides and also to reduce resolution of the analysis instrument. Both were accomplished with the surface-enhanced laser desorption/ionization (SELDI) technology -a complex solution of analytes was allowed to interact with an active surface (ion-exchange, hydro-

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Section: Biomarker Discovery In Urinementioning

confidence: 75%

Section: Biomarker Discovery In Urinementioning

confidence: 93%

Section: Biomarker Discovery In Urinementioning

confidence: 99%

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Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes

2005

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“…Initial studies involving application of urine proteomic analysis in distinguishing patients with acute rejection from patients with stable disease have identified common biomarkers (␤ 2 -microglobulin) with promising performance characteristics (sensitivity, specificity, and positive and negative predictive values) (15,16). Other studies in patients with IgA nephropathy suggest that urine protein patterns allow discrimination of patients with IgA nephropathy from healthy controls and patients with membranous nephropathy; importantly, treatment changes the patterns of protein excretion (17).…”

Section: Excreted Urinary Mediators As a Biomarker In Human Glomerulomentioning

confidence: 99%

Urine proteomics for the early diagnosis of acute glomerulonephritis: Has the time come?

Boumpas¹,

Kuroiwa²

2007

Arthritis & Rheumatism

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“…CE-MS was also applied to analysis of urinary polypeptides in case of IgA nephropathy [218]. The urine of patients with IgA nephropathy showed a different polypeptide pattern when compared to healthy controls.…”

Section: Urinary Proteinsmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy

Abstract: Proteomic analysis with CE-MS coupling permits fast and accurate identification and differentiation of polypeptide patterns in the urine of patients with IgAN, allowing differentiation from healthy controls and, probably, other renal diseases.

Cited by 211 publications

References 17 publications

Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes

Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes

Urine proteomics for the early diagnosis of acute glomerulonephritis: Has the time come?

Capillary electrophoresis of proteins 2003–2005

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