Urocortin-like immunoreactivity in the primary lymphoid organs of the duck (Anas platyrhynchos)

Luca, A. De; Squillacioti, C.; Pero, M.E.; Paino, S.; Langella, E.; Mirabella, N.

doi:10.4081/ejh.2009.167

Cited by 5 publications

(6 citation statements)

References 47 publications

(64 reference statements)

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“…The biological effects of CRH and UCNs are mediated by two distinct receptors, namely CRH receptor type 1 (CRHR1) and CRH receptor type 2 (CRHR2), respectively, which belong to the G‐protein‐coupled receptor superfamily of brain‐gut neuropeptides (Vita et al, ). Urocortin and its receptors have been shown to be associated with a variety of physiological functions and detected in digestive, cardiovascular, immune, endocrine and genital tracts (De Luca et al, ; Lee, Braden, Kang, & Rivier, ; Oki & Sasano, ; Squillacioti et al, , ; Squillacioti, Luca, Liguori, Paino, & Mirabella, ; Venkatasubramanian, Newby, & Lang, ; Yang et al, ). Specifically, UCN and CRHR expression has been observed in the testis of rat, mouse, human and dog (Lee et al, ; Squillacioti et al, ; Tao et al, ; Tezval et al, ), in the human prostate gland (Arcuri et al, ), and in the epididymis of the rat and alpaca (De Luca et al, ; Liguori et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…The biological effects of CRH and UCNs are mediated by two distinct receptors, namely CRH receptor type 1 (CRHR1) and CRH receptor type 2 (CRHR2), respectively, which belong to the G-protein-coupled receptor superfamily of brain-gut neuropeptides (Vita et al, 1995). Urocortin and its receptors have been shown to be associated with a variety of physiological functions and detected in digestive, cardiovascular, immune, endocrine and genital tracts (De Luca et al, 2009;Lee, Braden, Kang, & Rivier, 2011;Oki & Sasano, 2004;Squillacioti et al, , 2012Squillacioti, Luca, Liguori, Paino, & Mirabella, 2011;Venkatasubramanian, Newby, & Lang, 2010;Yang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Urocortinergic system in the epididymis of the normal and cryptorchid dogs

Squillacioti

Pelagalli

Luca

et al. 2019

Reprod Domestic Animals

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Cryptorchidism is associated with changes in the gonads and the spermatic duct system, which may cause infertility problems. Urocortin (UCN) is a corticotrophin‐releasing hormone (CRH)‐related peptide, which affects several functions of male genital organs. The aim of the present study was to investigate the expression of UCN and its receptors CRHR1 and CRHR2 using immunohistochemistry, western blotting and real‐time reverse transcription polymerase chain reaction in tissues collected from the epididymis of normal and cryptorchid dogs. The lumen of the cryptic epididymal duct was found to be relatively smaller than that of the normal one, and interstitial tissue was abundant in the cryptic epididymis. In addition, only a few spermatids were observed in the lumen of the epididymal duct. Results showed that UCN, CRHR2 and CRHR1 were expressed in tissues collected from normal and cryptic epididymal ducts. Urocortin‐ and CRHR2‐immunoreactivities (IRs) were detected in the principal cells of the caput, corpus and cauda of the normal and cryptic epididymides. CRHR1‐IR was detected in vascular smooth muscles and fibromuscular cells surrounding epididymal tubules of the normal and cryptorchid dogs. Expression levels of UCN and CRHR2 mRNA were higher in cryptic epididymal ducts than that in normal epididymal ducts. These results suggest that UCN and its receptors might play a role in regulating the maturation and storage of spermatozoa. These findings indicated that the expression of these proteins could be modulated by the cryptorchidism condition.

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Section: Introductionmentioning

confidence: 99%

“…The biological effects of CRH and UCNs are mediated by two distinct receptors, namely CRH receptor type 1 (CRHR1) and CRH receptor type 2 (CRHR2), respectively, which belong to the G-protein-coupled receptor superfamily of brain-gut neuropeptides (Vita et al, 1995). Urocortin and its receptors have been shown to be associated with a variety of physiological functions and detected in digestive, cardiovascular, immune, endocrine and genital tracts (De Luca et al, 2009;Lee, Braden, Kang, & Rivier, 2011;Oki & Sasano, 2004;Squillacioti et al, , 2012Squillacioti, Luca, Liguori, Paino, & Mirabella, 2011;Venkatasubramanian, Newby, & Lang, 2010;Yang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Urocortinergic system in the epididymis of the normal and cryptorchid dogs

Squillacioti

Pelagalli

Luca

et al. 2019

Reprod Domestic Animals

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“…CRHR1 exhibits high affinity for CRH and UCN, low for UCN 2 and no affinity for UCN 3; CRHR2 exhibits high affinity for UCNs and no affinity for CRH. The expression of UCN and CRH was identified in different organs belonging to digestive, cardiovascular, immune, endocrine and genital tracts (Oki and Sasano, ; De Luca et al., ; Venkatasubramanian et al., ; Yang et al., ; Lee et al., ; Squillacioti et al., , ). In the male genital tract, UCN and CRH receptors have been shown in the rat, mouse and human testis (Tao et al., ; Tezval et al., ; Lee et al., ), in human prostate (Arcuri et al., ) and in rat epididymis (De Luca et al., accepted in press).…”

Section: Introductionmentioning

confidence: 99%

Presence and Distribution of Urocortin and its Receptors in the Epididymis of Alpaca (Vicugna pacos)

Liguori

Squillacioti

Luca

et al. 2014

Anat. Histol. Embryol.

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Urocortin 1 (UCN) is a 40-amino acid peptide belonging to the corticotrophin-releasing hormone (CRH) family. The biological effects of this peptide are modulated by binding two G-coupled receptors named CRH receptor 1 (CRHR1) and CRH receptor 2 (CRHR2). CRHR2 has high affinity for UCN. The aim of the present study was to investigate the presence and distribution of UCN, CRHR1 and CRHR2 in the epididymis of the South America camelid Alpaca (Vicugna pacos) by Western blotting analysis and immunohistochemistry. Tissue extracts of the organ reacted with the anti-UCN, anti-CRHR1 and anti-CRHR2 antibodies, recognizing in all the cases a single specific protein band. UCN- and CRHR2-immunoreactivities (IRs) were found in the cytoplasm of the principal cells (PCs) of the caput epididymis. A prevalent supranuclear localization of granular-shaped positive material was observed. CRHR1-IR was observed in the fibromuscular stromal cells encircling the tubules and in the smooth musculature of the blood vessels throughout the three epididymal segments. In addition, in the cauda, CRHR1-IR was observed in some apical epithelial cells (ACs) which were morphologically similar to apical mitochondria-rich cells (AMRCs). These results suggest that UCN, CRHR1 and CRHR2 are expressed in the alpaca epididymis and that CRH-related peptides might play multiple roles in maturation and storage of spermatozoa.

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“…The production of several hormones has been described in the thymus of mammals and birds, as steroids, 23 glucocorticoids, 24–26 growth hormone, 27 POMC-derived peptides, 28–30 and neuropeptides. 31,32 …”

Section: Introductionmentioning

confidence: 99%

Effects of hypophyseal or thymic allograft on thymus development in partially decerebrate chicken embryos: expression of PCNA and CD3 markers

et al. 2010

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Changes in chicken embryo thymus after partial decerebration (including the hypophysis) and after hypophyseal or thymic allograft were investigated. Chicken embryos were partially decerebrated at 36–40 h of incubation and on day 12 received a hypophysis or a thymus allograft from 18-day-old donor embryos. The thymuses of normal, sham-operated and partially decerebrate embryos were collected on day 12 and 18. The thymuses of the grafted embryos were collected on day 18. The samples were examined with histological method and tested for the anti-PCNA and anti-CD3 immune-reactions. After partial decerebration, the thymic cortical and medullary compartments diminished markedly in size. Anti-PCNA and anti-CD3 revealed a reduced immunereaction, verified also by statistical analysis. In hypophyseal or grafted embryos, the thymic morphological compartments improved, the anti-PCNA and anti-CD3 immune-reactions recovered much better after the thymic graft, probably due to the thymic growth factors and also by an emigration of thymocytes from the same grafted thymus.

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Urocortin-like immunoreactivity in the primary lymphoid organs of the duck (Anas platyrhynchos)

Cited by 5 publications

References 47 publications

Urocortinergic system in the epididymis of the normal and cryptorchid dogs

Urocortinergic system in the epididymis of the normal and cryptorchid dogs

Presence and Distribution of Urocortin and its Receptors in the Epididymis of Alpaca (Vicugna pacos)

Effects of hypophyseal or thymic allograft on thymus development in partially decerebrate chicken embryos: expression of PCNA and CD3 markers

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