Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides

Langfield, Richard D.; Scarano, Frank J.; Heitzman, Mary E.; Kondo, Miwako; Hammond, Gerald B.; Neto, Catherine C.

doi:10.1016/j.jep.2004.06.013

Cited by 118 publications

(66 citation statements)

References 8 publications

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“…However, the dilution method is the recommended procedure for the quantitative assessment of these properties (Eloff, 1998;CLSI, 2008;Langfield et al, 2004). This study used the broth microdilution method, for a quantitative assessment of antimicrobial activity based on the value of MIC (CLSI, 2008).…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of medicinal plants from Northeastern Brazil

Ferreira¹,

Santiago²,

Langassner³

et al. 2013

J. Med. Plants Res.

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The aim of this work was to find how medicinal plants play an important role as source of new bioactive molecules. To evaluate the antifungal activity of 10 medicinal plants from northeastern Brazil, traditionally used as anti-infective agents. The activity of 30 crude extracts (water; ethanol:water, 1:1; acetone:water, 1:1) against four standard species of Candida yeasts (Candida albicans ATCC 90028, Candida dubliniensis ATCC 7289, Candida glabrata ATCC 2001 and Candida krusei ATCC 6258) was investigated by the Minimal Inhibitory Concentration (MIC), using the microdilution method and the working range used was from 1.95 to 1000 μg/mL. Extracts from leaves of Eugenia uniflora (Myrtaceae), stem bark of Caesalpinia ferrea (Caesalpinaceae) and leaves of Psidium guajava (Myrtaceae) showed significant activity against all yeasts evaluated. The best antifungal activities were achieved against C. glabrata and C. krusei by E. uniflora extract (MIC = 15.62) and followed by extracts from C. ferrea and P. guajava (MIC ranged from 15.62 to 250 µg/mL). E. uniflora also showed fungicidal properties against all yeasts, especially against Candida dubliniensis. This study identified plant species that may be candidates for the development of alternative treatments for candidiasis.

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Section: Discussionmentioning

confidence: 99%

Antifungal activity of medicinal plants from Northeastern Brazil

Ferreira¹,

Santiago²,

Langassner³

et al. 2013

J. Med. Plants Res.

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“…The antibacterial activity of the Cucumis sativus L. peel methanol and chloroform extract was analysed by well diffusion method 26 . Muller Hinton agar was prepared according to the standard procedure and 25 mL was poured into the plates and was allowed to solidify.…”

Section: Antibacterial Activitymentioning

confidence: 99%

In Vitro Antioxidant and Antimicrobial Properties of Cucumis Sativus L. Peel Extracts

John¹,

Priyadarshini²,

Monica³

et al. 2018

Int. Res. J. Pharm.

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The present study evaluated the phytochemicals, antioxidant and antimicrobial potential; and thin layer chromatographic studies of Cucumis sativus L. peel extracts. The total flavonoid content was assessed using Aluminium Chloride reagent method. The antioxidant potential was evaluated using in vitro assays viz DPPH radical scavenging assay, FRAP and Phosphomolybdenum assay. The antimicrobial activity was determined against Shigella flexneri, E coli, Staphylococcus aureus and Klebsiella pneumonia using Agar Well Diffusion method. Qualitative phytochemical analysis confirmed the presence of alkaloids, saponins, diterpenes, steroids and flavonoids. Total Flavonoid content in the Cucumis sativus L. peel was 3.50 µg/mg QE. Cucumis sativus L. peel showed highest radical scavenging activity of 71% at the concentration of 600 µg/mL. In determination of antioxidant activity via FRAP and Phosphomolybdenum assay cucumber peel extract showed highest absorbance of 0.80 and 0.94 respectively at the concentration of 300 µg/mL. The peel extracts inhibited the growth of all the tested pathogens by forming inhibition zones ranging from 11-21 mm. Thin layer chromatographic studies of the cucumber peel extracts constituted different coloured phytochemical compounds with different Rf values. This study highlights the pharmacological properties of Cucumis sativus L. peel.

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“…Uji bioassay untuk melihat aktivitas anti-V. harveyi dan anti-V. parahaemolyticus dari S. alba dan B. gymnnorrhiza dilakukan dengan teknik "High Throughput Screening" (Langfield et al, 2004;Ganju et al, 2008;Karuppusamy & Rajasekaran, 2009). Hasil fraksinasi dan ekstrak metanol dari kedua jenis mangrove tersebut ditimbang masing-masing sebanyak 5 mg dan 10 mg, kemudian dilarutkan dengan 1 mL DMSO (dimethylsulfoxide) 10% dalam tabung mikro volume 1,5 mL (Kasanah & Isnansetyo, 2013).…”

Section: Uji Aktivitas Anti-bakterial Secara Kualitatifunclassified

AKTIVITAS ANTIBAKTERI PENYEBAB VIBRIOSIS TERHADAP UDANG WINDU DARI EKSTRAK HERBAL MANGROVE Sonneratia alba DAN Bruguiera gymnorrhiza

Muliani

Tampangallo

Atmomarsono

2017

Jurnal Riset Akuakultur

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ABSTRAKPenelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak herbal mangrove Sonneratia alba dan Bruguiera gymnorrhiza terhadap penyebab penyakit vibriosis pada udang windu. Daun mangrove S. alba dan B. gymnorrhiza masing-masing diambil dari Kabupaten Maros dan Pangkep. Sampel daun dikeringanginkan selama dua minggu, ditepungkan, diekstraksi dengan metanol 80%, dan dievaporasi. Rendemen yang diperoleh dipartisi menggunakan dua jenis pelarut yaitu butanol dan dietileter. Uji bioassay dilakukan baik secara kualitatif maupun kuantitatif melalui penentuan minimum inhibition concentration (MIC) dan minimum bactericidal concentration (MBC PENDAHULUANPenyakit vibriosis atau penyakit udang berpendar merupakan salah satu jenis penyakit udang yang banyak menimbulkan kematian baik di tambak maupun di panti perbenihan. Vibriosis disebabkan oleh beberapa jenis bakteri vibrio seperti Vibrio harveyi, Vibrio parahaemolytiucus, Vibrio alginolyticus, Vibrio fischeri. Di antara beberapa jenis vibrio yang paling berbahaya adalah V. harveyi (Kannapiran et al., 2009) dan sampai saat ini penyakit ini masih merupakan kendala budidaya udang terutama di panti perbenihan.Berbagai upaya telah dilakukan untuk menanggulangi serangan penyakit pada budidaya udang, namun hingga saat ini kematian udang di tambak dan di panti perbenihan akibat serangan penyakit masih saja terus terjadi. Penggunaan bahan alam termasuk mangrove dan tanaman asosiasinya untuk penanggulangan penyakit di bidang perikanan mulai dirintis meskipun masih terbatas pada skala laboratorium di antaranya sebagai antibakteri (Velmurugan & Citarasu, 2010;Rajasekar et al., 2011;Rajeswari et al., 2012).

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Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides

Cited by 118 publications

References 8 publications

Antifungal activity of medicinal plants from Northeastern Brazil

Antifungal activity of medicinal plants from Northeastern Brazil

In Vitro Antioxidant and Antimicrobial Properties of Cucumis Sativus L. Peel Extracts

AKTIVITAS ANTIBAKTERI PENYEBAB VIBRIOSIS TERHADAP UDANG WINDU DARI EKSTRAK HERBAL MANGROVE Sonneratia alba DAN Bruguiera gymnorrhiza

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