ObjectiveTo evaluate the penetration of hydrogen peroxide (HP) into the pulp chamber and the color change of different bleaching varnishes in low concentrations used for at‐home bleaching.Materials and MethodsNinety healthy premolars were used, randomly distributed into nine groups (n = 10) according to bleaching varnish (PL, PolaLuminate; VS, VivaStyle Paint On Plus; CA, Cavex Bite&White whitening pen and; AW AlignerWhite) and time (10 and 30 min), and a control group (no bleaching). The penetration of HP was evaluated by UV–Vis spectroscopy. To evaluate the color change (ΔEab, ΔE00, ΔWID) a digital spectrophotometer was used (α = 0.05).ResultsThe AW group in 10 min and the control group showed similar and lower HP penetration in the pulp chamber when compared to the other groups (p = 0.003). Increasing the application time to 30 minutes elevated the amount of HP inside the pulp chamber for all groups (p = 0.003), except for PL (p > 0.05). When applied for 30 min all bleaching varnishes showed higher color change (ΔWID) when compared to 10 min (p = 0.04).ConclusionsFor all bleaching varnishes evaluated, PolaLuminate applied for 30 min showed lower penetration into the pulp chamber and higher bleaching effects.Clinical SignificanceThe use of bleaching varnishes seems promising for teeth bleaching, but it varies according to user product and protocol.