Use of an Enzyme-Linked Immunosorbent Assay to Measure Antibody Levels in Turkey Breeder Hens, Eggs, and Progeny Following Natural Infection or Immunization with a Commercial Bordetella avium Bacterin

Abstract

Twelve large white turkey hens were immunized with a commercially available Bordetella avium bacterin. Hens and eggs were tested using an enzyme-linked immunosorbent assay (ELISA) to determine the response to the bacterin. Three hundred poults were then obtained from two commercial flocks, the hens of one flock having been immunized with the same bacterin used on the group of 12 turkeys. Titers of the poults were monitored for 7 weeks, and poults were challenged by exposure to infected poults at 1, 7, 14, and 21 days post-hatch. Hens produced an antibody response following immunization, with a parallel antibody response being detected in eggs. Maternal antibodies were present in poults from immunized hens. Poult titers declined to near the level of poults from unimmunized hens by 14 days of age. Poults from immunized hens challenged at 1 and 7 days were resistant to development of clinical disease and gross lesions, whereas all poults from unimmunized hens exhibited clinical signs and gross lesions. After 14 days, the resistance of both groups to development of clinical disease, became near equal, neither group being affected as severely as the unimmunized hens challenged at days 1 and 7. Six commercial turkey breeding flocks and their progeny that had not been vaccinated for B. avium and had no history of B. avium infection were evaluated with the B. avium ELISA. There were variations between the flocks, with poult titers reflecting those found in the hens.

Hindilerde Bordetella avium antikorlarının enzyme-linked immunosorbent assay, mikroaglutinasyon ve lam aglutinasyon testleri ile belirlenmesi üzerine karşılaştırmalı bir araştırma

“…ELISA oldukça güvenilir bir teşhis yöntemidir ve pek çok laboratuarda referans test olarak B. avium'a karşı oluşan antikorların belirlenmesinde kullanılmaktadır. Ancak ELISA kitlerinin fiyatının yüksek olması kitin kullanılabilirliği açısından önemli bir dezavantajdır (2,9,12). Bununla birlikte henüz ticari olarak B. avium Lam Aglütinasyon Test (LAT) antijeni geliştirilmemiştir.…”