Use of Blood-Glucose Test Strips for Introducing Enzyme Electrodes and Modern Biosensors

Wang, Joseph; Maccà, Carlo

doi:10.1021/ed073p797

Cited by 18 publications

(21 citation statements)

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“…Wang and Macca [3] did some amperometric studies on carbon test strips. But, they did not do any works on gold test strips nor any works on the effect of electrode design on glucose measurement.…”

Section: Introductionmentioning

confidence: 99%

Design of Electrodes on Gold Test Strips for Enhanced Accuracy in Glucose Measurement

et al. 2019

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This paper reviews the practical process of designing the electrodes on electroless immersion gold-plated test strips for glucose measurement. We have found that great care must be taken on determining the width and length of the working and reference electrodes, to yield the optimum performance on electroless immersion gold-plated glucose test strips for glucose measurement. Preferably, a width of at least 0.6 mm and a length of at least 24 mm are recommended to prevent variance in electrode resistance during mass production of electroless immersion gold-plated glucose test strips, in order to enhance the accuracy of glucose measurement. This paper also recommended that, if two different geometric sizes of test strips are going to be used on the same glucose meter, both test strips must use different lengths and widths on the working and reference electrodes to yield the same electrical resistance and hence similar electrochemical performance.

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Section: Introductionmentioning

confidence: 99%

Design of Electrodes on Gold Test Strips for Enhanced Accuracy in Glucose Measurement

et al. 2019

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“…Some bioanalytical protocols based on the application of biosensors have been presented: potentiometric detection of urea, amperometric detection of glucose based on the application of redox mediators [8] and depletion of dissolved oxygen [9], and detection of hydrogen peroxide concentration by an oxygen electrode [5]. The application of soluble redox mediators and/or detection of reaction substrates/products were applied in each case.…”

mentioning

confidence: 99%

Surface plasmon resonance label‐free monitoring of antibody antigen interactions in real time

Kaušaitė

Dijk²,

Castrop³

et al. 2007

Biochem Molecular Bio Educ

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Detection of biologically active compounds is one of the most important topics in molecular biology and biochemistry. One of the most promising detection methods is based on the application of surface plasmon resonance for label-free detection of biologically active compounds. This method allows one to monitor binding events in real time without labeling. The system can therefore be used to determine both affinity and rate constants for interactions between various types of molecules. Here, we describe the application of a surface plasmon resonance biosensor for label-free investigation of the interaction between an immobilized antigen bovine serum albumin (BSA) and antibody rabbit anti-cow albumin IgG1 (anti-BSA). The formation of a self-assembled monolayer (SAM) over a gold surface is introduced into this laboratory training protocol as an effective immobilization method, which is very promising in biosensing systems based on detection of affinity interactions. In the next step, covalent attachment via artificially formed amide bonds is applied for the immobilization of proteins on the formed SAM surface. These experiments provide suitable experience for postgraduate students to help them understand immobilization of biologically active materials via SAMs, fundamentals of surface plasmon resonance biosensor applications, and determination of non-covalent biomolecular interactions. The experiment is designed for master and/or Ph.D. students. In some particular cases, this protocol might be adoptable for bachelor students that already have completed an extended biochemistry program that included a background in immunology.

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“…An at-home experiment for determining glucose in beverages using a commercial glucometer was developed. This experiment was adapted from a report that used blood-glucose test strips for introducing enzyme electrodes and modern biosensors to undergraduate students [ 3 ]. An overview and background information on amperometric blood-glucose meters and test strips are provided on the ASDL website [ 4 ].…”

Section: Remote Experiments For An Instrumental Analysis Laboratorymentioning

confidence: 99%

Hands-on experiences for remotely taught analytical chemistry laboratories

et al. 2021

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Use of Blood-Glucose Test Strips for Introducing Enzyme Electrodes and Modern Biosensors

Cited by 18 publications

References 0 publications

Design of Electrodes on Gold Test Strips for Enhanced Accuracy in Glucose Measurement

Design of Electrodes on Gold Test Strips for Enhanced Accuracy in Glucose Measurement

Surface plasmon resonance label‐free monitoring of antibody antigen interactions in real time

Hands-on experiences for remotely taught analytical chemistry laboratories

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