Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon-contaminated and bioremediated soils

Płaza, Grażyna; Zjawiony, Ireneusz; Banat, Ibrahim M.

doi:10.1016/j.petrol.2005.10.005

Cited by 181 publications

(134 citation statements)

References 25 publications

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“…The oil spreading method is rapid and easy to carry out, requires no specialized equipment and just a small volume of sample (Płaza et al, 2006). It can be applied when the activity and quantity of biosurfactant is low.…”

Section: Oil Displacement Testmentioning

confidence: 99%

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

109

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-Pseudomonas aeruginosa TMN was used to produce rhamnolipid (RL) from a variety of carbon and nitrogen substrates. The most favorable carbon sources for RL production were glucose and glycerol (both at 40 g/L), giving a RL yield of 0.3 and 0.25 g/L, respectively. Meanwhile, sodium nitrate appeared to be the preferable nitrogen source, resulting in a RL production of 0.34g/L. Rhamnolipid production from P. aeruginosa TMN was affected by temperature, pH and agitation rate, with 37 °C, pH 7 and 200 rpm agitation favorable for rhamnolipid production. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) and electro spray ionization-mass spectrometry (ESI-MS) analyses indicated that the purified product contained one type of commonly found rhamnolipid, which is L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate. The rhamnolipid product can reduce the surface tension of water to 34 mN/m with a critical micelle concentration of nearly 18.75 mg/L and emulsified kerosene by 46%. P. aeruginosa TMN strain is a potential source of rhamnolipid biosurfactant, which could be used for the development of bioremediation processes in the marine environment.

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Section: Oil Displacement Testmentioning

confidence: 99%

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

109

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“…This test was carried out as prescribed by Plaza et al 22 . In this method, a drop of supernatant from each bacterial isolate was taken onto a sterile glass slide.…”

Section: Drop Collapse Testmentioning

confidence: 99%

“…Plaza et al 22 proposed the drop collapse technique as a quantitative method to assess biosurfactant production 30 . However the present study involves the use of this technique for qualitative estimation.…”

Section: Drop Collapse Test Oil Spreading Assay and Emulsification Amentioning

confidence: 99%

Biodegradation of Crude Oil Using Marine <i>Bacillus species</i> from Vadinar Coast, Gujarat, India

Mulani¹,

Fulke²,

DeSouza³

et al. 2017

Current Science

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The marine environment is open to large sources of toxic organic waste in the form of accidental oil spills. Therefore, it is important to study microbial degradation processes that help reduce the damage caused to the environment. Universally, oil spills produce enormous public anxiety and highlight the need for costeffective, indigenous and environmentally acceptable bioremediation technologies. In recent times, advanced remedial techniques have been opted, such as solidifying, skimming, controlled burning and bioremediation. The present study aimed to isolate crude oil-degrading marine bacteria from Vadinar coastal area of Gujarat, India. Among seven isolates, three potential bacterial strains were chosen for crude oil and petroleum hydrocarbon (PHC) degradation, which were analysed by UV spectrophotometric and fluorometric analysis. These bacterial cultures were verified by 16S rRNA gene sequencing and identified as Bacillus species. Phylogenetic analysis was carried out to confirm the evolutionary relationship with existing oil-degrading species. In the present study, drop collapse, oil spreading and emulsification assay were performed to detect biosurfactant production. Bacillus sp. NM1 KT354277 was capable of degrading 50% of PHCs at the end 72 h for one week under rotary incubation in ONR7a medium. Among the studied strains, Bacillus sp. NM3 KT354278 showed promising lipase activity, viz. 60.72 and 61.19 U ml -1 for 2% of olive oil and tributyrin respectively. Thus, the present study explores indigenous marine isolates that could be utilized as a potential alternative for oilspill remediation in future.

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“…It produced an extracellular biosurfactant called Cladosan after twenty five days that reduced the surface tension of the aqueous phase from 72 mN/m to 50 mN/m and, after being partially purified, reduced the tension to 40 mN/m (Muriel et al, 1996). Plaza et al (2006) investigated the biosurfacatant/ bioemulsifier productions to sixteen species of bacteria, isolated from contaminated soil with petroleum hydrocarbons. The experiments were carried out in liquid cultures containing crude oil under thermophilic conditions.…”

Section: Culture Medium Investigation For Bioemulsifier/ Biosurfactanmentioning

confidence: 99%

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

Souza

Salgueiro

Albuquerque

2012

Braz. J. Chem. Eng.

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-The objective of this work was to investigate, on a flask scale, the production of bioemulsifiers by Yarrowia lipolytica in the presence of sea water, supplemented with nitrogen and phosphate sources, using diesel oil as substrate. A full 2 4 factorial design was conducted to investigate the effects and interactions of the nutrient concentrations (diesel oil, urea, ammonium sulfate and monobasic potassium phosphate) on the response variables: emulsification activity and surface tension of the cell-free cultures. High emulsification activities (> 5,4 UEA) were determined after 168 h in all the experiments. The interactions among diesel oil, urea and monobasic potassium phosphate favored the emulsification with statistical significance. A correlation between the increase of emulsification activity and the reduction of surface tension was not identified.

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Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon-contaminated and bioremediated soils

Cited by 181 publications

References 25 publications

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Biodegradation of Crude Oil Using Marine <i>Bacillus species</i> from Vadinar Coast, Gujarat, India

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

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