Use of extracellular extracts of lactic acid bacteria and bifidobacteria for the inhibition of dermatological pathogen Staphylococcus aureus

Hor, Yan-Yan; Liong, Min‐Tze

doi:10.1016/j.dsi.2014.03.001

Cited by 68 publications

(42 citation statements)

References 17 publications

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“…The bacterial load and the numbers of the infected patients with bacteria remarkably reduced in response to oral administration probiotic capsules. This is in line with prior studies which detected antibacterial activity of Lactobacilli and Bifidobacteria in the laboratory against common disease pathogens such as S. typhimurium, E. coli and S. aureus that proved what Hor and Liong (2014) and Hütt (2006) noted. The identification with VITEK 2 system as a precise automated technique was used to identify the isolated pathogens into P. aeuroginosa, E. coli, S. lentus, S. aureus, K. pneumonia.…”

Section: Discussionsupporting

confidence: 91%

Lactobacillus acidophilus and Bifidobacteria spp having antibacterial and antiviral effects on chronic HCV infection

Allam¹,

Salem²,

Elbatae³

et al. 2019

Afr. J. Microbiol. Res.

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Hepatitis C virus is a serious hepatic disease that could be developed into hepatocellular carcinoma. Previously, some probiotic strains showed a natural therapeutic activity against the fatty liver disorder. Therefore, it could make sense to evaluate the antiviral and antibacterial responses to probiotics as Lactobacillus acidophilus and Bifidobacteria spp. in patients with chronic hepatitis C virus. Twenty (20) patients with chronic hepatitis C (both gender in age 47± 5 years) were treated with capsule that contains probiotics (L. acidophilus and Bifidobacterium spp.). They administered one capsule per day for a month before HCV treatment; blood and urine samples were collected before and after the given treatment and they were processed for a quantitative estimation of HCV by PCR, identification of bacteria by VITEK2 system and 16S r RNA gene sequencing assay. Moreover, the estimation of antibacterial activity of probiotics by antibiotic sensitivity test, counts of leukocytes, CD3 + T cells and CD56 + natural killer cells by flowcytometry, DGAT1 by ELISA. Administration of probitics enhanced the treatment response rate to HCV treatments pegylated IFN-α and ribavirin by 25% and showed an antibacterial activity against five species of the most common infectious bacteria in chronic HCV patients which were identified by this study. CD3 + cells counts and CD56 + natural killer cells were increased. L. acidophilus and Bifidobacterium spp. can act as a supportive supplement with antiviral and antibacterial activities; we recommend Probiotics side by side with HCV treatments.

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Section: Discussionsupporting

confidence: 91%

Lactobacillus acidophilus and Bifidobacteria spp having antibacterial and antiviral effects on chronic HCV infection

Allam¹,

Salem²,

Elbatae³

et al. 2019

Afr. J. Microbiol. Res.

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“…Accordingly, Al Kassaa et al (2014) showed that L. fermentum CMUL054 and L. plantarum CMUL140 were active against S. aureus ATCC33862 [23]. Moreover, Hor and Liong (2014) analyzed 87 lactic acid bacteria strains and three strains of bifidobacteria, and found that all strains inhibited the growth of S. aureus by 0.5 to 34.2 %; L. fermentum and L. plantarum , which were isolated from milk, inhibited growth by around 20 % [24]. Although inhibition of S. aureus by lactic bacteria has been reported in many studies [23–26], few of them have calculated the MIC from extracellular bacterial extracts.…”

Section: Resultsmentioning

confidence: 99%

“…We did not observe statistically significant differences between treatments with 90 % (2.25 mg∙mL −1 ) or 70 % of the MIC (1.75 mg∙mL −1 ) for this strain. In a previous report, the supernatant of L. bulgaricus FTDC8611 inhibited significantly S. aureus biofilm, an effect attributed to organic acids [24]. Also, Ait Ouali et al(2014) demonstrated that L. pentosus LB3F2 had antimicrobial and antibiofilm activity against S. aureus SA3 [29].…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Staphylococcus aureus biofilm by Lactobacillus isolated from fine cocoa

et al. 2016

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BackgroundBiofilm production represents an important virulence and pathogenesis factor for Staphylococcus aureus. The formation of biofilms on medical devices is a major concern in hospital environments, as they can become a constant source of infection. Probiotic bacteria, such as Lactobacillus fermentum and L. plantarum, have been found to inhibit biofilm formation; however little is known about the underlying mechanism. In this study, we tested the activity of supernatants produced by L. fermentum TCUESC01 and L. plantarum TCUESC02, isolated during the fermentation of fine cocoa, against S. aureus CCMB262 biofilm production. We measured inhibition of biofilm formation in vitro and analyzed biofilm structure by confocal and electronic microscopy. Additionally, we quantified the expression of S. aureus genes icaA and icaR involved in the synthesis of the biofilm matrix by real-time PCR.ResultsBoth Lactobacillus supernatants inhibited S. aureus growth. However, only L. fermentum TCUESC01 significantly reduced the thickness of the biofilm, from 14 μm to 2.83 μm (at 18 mg∙mL−1, 90 % of the minimum inhibitory concentration, MIC), 3.12 μm (at 14 mg∙mL−1, 70 % of the MIC), and 5.21 μm (at 10 mg∙mL−1, 50 % of the MIC). Additionally, L. fermentum TCUESC01 supernatant modulated the expression of icaA and icaR.Conclusions L. fermentum TCUESC01 reduces the formation of S. aureus biofilm under subinhibitory conditions. Inhibition of biofilm production probably depends on modulation of the ica operon.

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“…The impact of probiotics can be attributed to their molecular components, and their effects can be mediated by either live or dead cells. Nevertheless, scientific evidence implies that inactivated microbes and their metabolites positively affect human and animal health (Adams 2010; Hor and Liong, 2014; Kataria et al , 2009). Accordingly, products designed with heat-killed strain Lactobacillus acidophilus ( L. acidophilus ) from human origin Lactéol strain (Coconnier et al , 1997) have been imported and marketed in China since early 1998 (PUMC Pharmaceutical Co., Ltd).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cronobacter sakazakii by Lactobacillus acidophilus n.v. Er2 317/402

Charchoghlyan¹,

Kwon²,

Hwang³

et al. 2016

Korean Journal for Food Science of Animal Resources

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Lactobacillus acidophilus n.v. Er2 317/402 strain Narine is known as a health beneficial functional probiotic culture and supplementary source of nutrition for newborns. In this study, in vitro antimicrobial activities of Narine-lyophilized (Narine-L), Narine-heat treated (Narine-HT), and Narine crude cell-free extract (Narine-CCFE) were evaluated against pathogen Cronobacter sakazakii (C. sakazakii) in agar as well as in a reconstituted powdered infant formula (RPIF) model. Inhibition zones of 30 mg Narine-L and Narine-HT were both 150 U, whereas inhibition zone of 30 mg Narine-CCFE was 200 U. Narine-L (1 g) and Narine-HT (1 g) were added to 10 mL of artificially contaminated RPIF, respectively, containing 100 μL of C. sakazakii (1.62×108 colony forming unit (CFU)/mL). After treatment with Narine-L and Narine-HT for 3 h and 6 h at 37℃, less than ≤107 CFU/mL of C. sakazakii was detected in RPIF. Without Narine-L and Narine-HT treatment, the population of C. sakazakii increased up to 5.36×109 CFU/mL after 6 h. Examination by transmission electron microscopy confirmed C. sakazakii cells were damaged by Narine-CCFE. Thus, employing Narine culture as a natural and safe bio-preservative may protect infants from C. sakazakii.

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Use of extracellular extracts of lactic acid bacteria and bifidobacteria for the inhibition of dermatological pathogen Staphylococcus aureus

Cited by 68 publications

References 17 publications

Lactobacillus acidophilus and Bifidobacteria spp having antibacterial and antiviral effects on chronic HCV infection

Lactobacillus acidophilus and Bifidobacteria spp having antibacterial and antiviral effects on chronic HCV infection

Inhibition of Staphylococcus aureus biofilm by Lactobacillus isolated from fine cocoa

Inhibition of Cronobacter sakazakii by Lactobacillus acidophilus n.v. Er2 317/402

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