Use of extraction columns for the isolation of desonide and parabens from creams and ointments for high-performance liquid chromatographic analysis

Nguyen, Thuy T.; Kringstad, Randi; Rasmussen, Knut

doi:10.1016/s0021-9673(01)93500-9

Cited by 13 publications

(5 citation statements)

References 7 publications

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“…Extraction of additives from various matrices (food, cosmetics and pharmaceutical products) has been previously achieved by solvent or solid‐phase extraction [2–5]. However, most of them are time‐consuming and poor recoveries occur for some additives.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 21 preservatives in cosmetics by ultra performance liquid chromatography

Wang

et al. 2008

Intern J of Cosmetic Sci

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An ultra performance liquid chromatography (UPLC) method has been developed for the simultaneous determination of 21 preservatives: 2-methyl-4-isothiazoline-3-ketone, bronopol, 5-chloro-2-methyl-4-isothiazoline-3-ketone, benzyl alcohol, 2-phenoxyethanol, methyl-p-hydroxy benzoate, ethyl-p-hydroxy benzoate, methyl benzoate, 4-hydroxybenzoic acid iso-propyl ester, propyl-p-hydroxy benzoate, 4-chloro-3-methylphenol, ethyl benzoate, 2-phenylphenol, 4-hydroxybenzoic acid iso-butyl ester, butyl-p-hydroxy benzoate, 4-chloro-3,5-dimethylphenol, phenyl benzoate, 2,4-dichloro-3,5-dimethylphenol, 2-benzyl-4-chlorophenol, triclocarban and triclosan in cosmetics. A Waters ACQUITY UPLC BEH C(18) column was used with 0.1% formic acid solution as the mobile phase under the condition of gradient elution. Preservatives were extracted with methanol by ultrasonicator, and then they were analysed by UPLC-PDA detector. All these preservatives were baseline separated in 8.5 min. The pre-treatment method of samples and the chromatographic condition of analysis were critically examined in this study. The recoveries ranged from 90.5 to 97.8%, with RSD values below 3.2%, and all correlation coefficients (r) were no less than 0.9997. Thus, this method could be used for analysing the preservatives in cosmetic products.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 21 preservatives in cosmetics by ultra performance liquid chromatography

Wang

et al. 2008

Intern J of Cosmetic Sci

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“…Desonide has no inclusion in any pharmacopoeia [55]; however the determination of desonide in biological samples had already been performed by HPLC [56,57], as well as ear drops [58], lotion hair [55,59], creams, and ointments [49,60], but never was encapsulated or quantified in nanoparticles. These colloidal drug carrier systems such as nanocapsules are analytical challenge due to the variety of compounds in the formulation and also the need to detect small amounts of drugs [61].…”

Section: Discussionmentioning

confidence: 99%

“…. Desonide in the formulation was quantified by high performance liquid chromatography (HPLC) using a method adapted from Nguyen and coworkers [49]. The chromatographic system was YL-Clarity5 model YL9100 HPLC System, bomb model YL9110, and detector UV model YL 916, using a column Lichropher5 100 RP-18, 250 mm, 4 mm, 5 m, Merck5.…”

Section: Chromatographic Systemmentioning

confidence: 99%

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Antonow

Lorenzoni

Barbosa

et al. 2016

Advances in Materials Science and Engineering

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Desonide is a topical corticosteroid that has been used for more than 30 years; however, its prolonged use can induce several side effects, affecting dermis and epidermis. The present work consists of development desonide-loaded nanocapsule suspensions (D-NC) using different polymers (Eudragit S100® or Eudragit L100®) and desonide-loaded lipid-core nanocapsules (D-LNC). They were formulated by interfacial deposition using the preformed polymer method and all formulations showed negative zeta potential and adequate nanotechnological characteristics (particle size 161–202 nm, polydispersity index < 0.20). Simple and sensitive methods using high-performance liquid chromatography (HPLC) were developed to quantify desonide in LNC and to study its release kinetics. The method was linear, specific, precise, and exact and therefore can be applied in quantification of D-NC and D-LNC. We evaluatedin vitromethods for drug release (dissolution, Franz diffusion cells, and dialysis sac) and we use mathematical models (monoexponential, biexponential, and Korsmeyer-Peppas) to show release kinetics from this system.

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“…Although it has been used for several decades, there are no official pharmacopeia methods to control the content of this active pharmaceutical ingredient in the topical formulations currently used (Sweetman 2007). Moreover, the analytical methods previously published regarding desonide containing formulations (DiPietra et al 1996;Nguyen et al 1988;Nguyen, Kringstad, and Rasmussen 1986;USP 1996) focus only on the non-simultaneous quantification of desonide (Nguyen et al 1988;USP 1996) and two preservatives, methylparaben and propylparaben (Nguyen et al 1986), or binary mixtures (DiPietra et al 1996). However, no methodology is available to quantify simultaneously desonide and different preservatives (such as antioxidant agents and other anti-microbial agents) included in several emulsions commercially available.…”

Section: Introductionmentioning

confidence: 99%

Development of an HPLC Assay Methodology for a Desonide Cream with Chemometrics Assisted Optimization

et al. 2012

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A new reversed-phase liquid chromatographic method for the separation and simultaneous quantification of desonide, sorbic acid, methylparaben, propyl gallate, and the major degradation product of desonide in a hydrophilic cream was developed with the aid of experimental design, resolution loss functions, and chemometrics methods. A strategy involving a screening phase and a fractional factorial design revealed the most influent chromatographic variables (pH and organic solvent content). The arc tangent resolution function was adopted as the optimization loss function. These variables were further optimized using a central composite design. Multivariate curve resolution and partial least squares regression were tested to optimize the chromatographic run time. The latter revealed to be superior in terms of precision and allowed the validation of a method with a total run time 3 times lower (approximately 8 min). The experimental design and chemometrics models enabled an efficient use of time and resources in predicting the optimum separation conditions for the desonide formulation. The validation of the resulting method according to the current ICH guidelines confirmed its selectivity, linearity, accuracy, and precision.

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Use of extraction columns for the isolation of desonide and parabens from creams and ointments for high-performance liquid chromatographic analysis

Cited by 13 publications

References 7 publications

Simultaneous determination of 21 preservatives in cosmetics by ultra performance liquid chromatography

Simultaneous determination of 21 preservatives in cosmetics by ultra performance liquid chromatography

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Development of an HPLC Assay Methodology for a Desonide Cream with Chemometrics Assisted Optimization

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