Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration

Builles, Nicolas; Janin-Manificat, H.; Malbouyres, Marilyne; Justin, V.; Rovere, M.R.; Pellegrini, Graziella; Torbet, J.; Hulmes, David J.S.; Burillon, C.; Damour, O.; Ruggiero, Florence

doi:10.1016/j.biomaterials.2010.07.066

Cited by 76 publications

(56 citation statements)

References 41 publications

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“…Compared to earlier studies with poorer retention with techniques requiring extensive suturing [26,27], the FLISK technique resulted in a 100% implant retention rate in 15 rabbit corneas. The procedure maintained epithelial integrity and resulted in rapid wound healing without inflammation and without the use of surgical sutures, thereby avoiding the potentially damaging effects of the epithelial wound healing response [37].…”

Section: Discussionmentioning

confidence: 96%

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Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Koulikovska

Rafat

Petrovski

et al. 2015

Tissue Engineering Part A

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Severe shortage of donor corneas for transplantation, particularly in developing countries, has prompted the advancement of bioengineered tissue alternatives. Bioengineered corneas that can withstand transplantation while maintaining transparency and compatibility with host cells, and that are additionally amenable to standardized, low-cost mass production, are sought. In this study, a bioengineered porcine construct (BPC) was developed to function as a biodegradable scaffold to promote corneal stromal regeneration by host cells. Using high purity medical-grade type I collagen, high 18% collagen content and optimized EDC-NHS crosslinker ratio, BPCs were fabricated into hydrogel corneal implants with over 90% transparency and fourfold increase in strength and stiffness compared to previous versions. Remarkably, optical transparency was achieved despite the absence of collagen fibril organization at the nanoscale. In vitro testing indicated the BPC supported confluent human epithelial and stromal-derived mesenchymal stem cell populations. With a novel femtosecond laser-assisted corneal surgical model in rabbits, cell-free BPCs were implanted in vivo in the corneal stroma of 10 rabbits over an 8 week period. In vivo, transparency of implanted corneas was maintained throughout the postoperative period, while healing occurred rapidly without inflammation and without the use of postoperative steroids. BPC implants had a 100% retention rate at 8 weeks, when host stromal cells began to migrate into implants. Direct histochemical evidence of stromal tissue regeneration was observed by means of migrated host cells producing new collagen from within the implants. This study indicates that a cost-effective BPC extracellular matrix equivalent can incorporate cells passively to initiate regenerative healing of the corneal stroma, and is compatible with human stem or organ-specific cells for future therapeutic applications as a stromal replacement for treating blinding disorders of the cornea.3

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Section: Discussionmentioning

confidence: 96%

“…In earlier studies, stromal cell invasion of implanted biomaterials has been shown, but the production of new collagen by these invading cells has not been convincingly demonstrated [8,23,24,[26][27][28]. The BPC also supported human corneal epithelial cell growth and a human corneal stroma-derived mesenchymal stem cell population in vitro.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Koulikovska

Rafat

Petrovski

et al. 2015

Tissue Engineering Part A

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“…The distance of the gratings appears to be more important than the attributes of the material [Koo et al, 2011]. Cell alignment can also be induced using scaffolds that consist of aligned fibers as a cell culture substrate [Builles et al, 2010;Wu et al, 2012]. Wu et al [2012] recently illustrated that a keratocyte phenotype (due to serum-free culture conditions) is not sufficient to produce globally aligned stromal tissues.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Vitamin C-Induced Cell Sheets Formed from Primary and Immortalized Human Corneal Stromal Cells for Tissue Engineering Applications

Grobe

Reichl²

2013

Cells Tissues Organs

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The purpose of this study was to compare the ability of primary human corneal stromal cells (HuFib cells) and SV40-immortalized human corneal keratocytes (HCK cells) to synthesize their own extracellular matrix induced by vitamin C supplementation. Therefore, the amount of collagen secreted and resulting biomechanical properties based on the culture duration were assessed. Cells were cultivated for several weeks with or without vitamin C. The amount of collagen secreted by the cells was quantified based on the culture duration. Cell viability was simultaneously determined via the MTT assay. Collagen secretion was increased as a result of vitamin C supplementation. The effect was stronger in primary cells. In addition, vitamin C supplementation had a positive effect on HuFib cell viability. Vitamin C supplementation induced the formation of detachable cell sheets in both primary and immortalized cells. The biomechanical properties of the sheets were evaluated using a static material testing machine, and the ultrastructure of the cell sheets was examined using scanning electron microscopy. The cell sheets formed from HuFib cells had a higher percentage of light transmission between 400 and 800 nm and were superior in terms of E-modulus and ultimate strength testing. Indirect immunofluorescence and Western blot confirmed the presence of collagen type I in the HuFib and HCK cell cultures. Stimulating secretion of the extracellular matrix in corneal stromal cells is a promising approach for corneal stroma reconstruction for tissue engineering applications.

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“…Type I collagen fibrils are induced to align orthogonally in a high magnetic field, which mimic the fibril organization of corneal stroma and the transparency can be greatly improved by adding proteoglycans [15,16]. Such scaffolds allow the in vitro construction of human hemi-corneas comprises of stroma and epithelium by seeding human keratocytes and limbal stem cell derived epithelial cells [15,16]. Collagen vitrigel which is made of type I collagen is used to culture epithelial cells, keratocytes and endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Matrix is an Important Component of Limbal Stem Cell Niche

Mei

González

Deng

2012

JFB

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Extracellular matrix plays an important role in stem cell niche which maintains the undifferentiated stem cell phenotype. Human corneal epithelial stem cells are presumed to reside mainly at the limbal basal epithelium. Efforts have been made to characterize different components of the extracellular matrix that are preferentially expressed at the limbus. Mounting evidence from experimental data suggest that these components are part of the stem cell niche and play a role in the homeostasis of limbal stem cells. The extracellular matrix provides a mechanical and structural support as well as regulates cellular functions such as adhesion, migration, proliferation, self-renewal and differentiation. Optimization of the extracellular matrix components might be able to recreate an ex vivo stem cell niche to expand limbal stem cells.

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Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration

Cited by 76 publications

References 41 publications

Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Characterization of Vitamin C-Induced Cell Sheets Formed from Primary and Immortalized Human Corneal Stromal Cells for Tissue Engineering Applications

Extracellular Matrix is an Important Component of Limbal Stem Cell Niche

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