Abstract. Despite the vine adaptation to different soil types, most are found in soils with low nutrient supply, requiring corrections. The organic fertilizer facilitates the absorption of nutrients and improve soil structure. The objective was to evaluate the effect of organic fertilization with bovine and ovine manure on growth and totalchlorophyll content of 'Merlot' vines grafted on three different rootstocks. The experiment was conducted by the Nucleus of Study, Research and Extension in Oenology (NEPE 2 ) with the variety 'Merlot' VCR1 clone. The treatments were applied in the rootstock '161.49' 420-A 'and' Paulsen 1103', and used manure of bovine and ovine origin, totaling 12 treatments. Were evaluated the plant height (cm) and total chlorophyll (Falker clorofiLOG R ). The treatments at the rootstock 'Paulsen 1103' showed greater height of 'Merlot' clone VCR1, followed by the rootstock '161.49' and '420A'. But the treatments with the rootstock 'Paulsen 1103' showed less chlorophyll in the leaves of 'Merlot' clone VCR1 compared to other treatments. Preliminarily itwas concluded that the use of manurefrom bovine and ovine origin on the vine 'Merlot' clone VCR1 on the rootstocks 'Paulsen 1103', '420A' and '161.49', present in the leaves higher total chlorophyll content.