Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

Abdalla

Egyptian Academic Journal of Biological Sciences. A, Entomology

et al. 2020

Radiation technology is widely used to produce changes in biosystems. The goal of this work is to determine the variation induced in male parents and F1 Ceratitiis capitata when gamma-irradiated as pupae using substerilising doses (50 and 70 Gy and sterilising dose 90 Gy. Biological studies showed that the percent adult emergence of irradiated pupae decreased as the dose increased and also the percent egg hatchability decreased as the dose increased, also percent pupation and percent emergence of F1 decreased as the dose increased. Comparing DNA (using RAPD-PCR) of untreated adults, adults irradiated with sterilising dose of 90 Gy and parents irradiated with substerile dose (50 and 70 Gy) and their resultant F1. The results revealed that some extra bands appeared and others disappeared, as a result of irradiation. The appearance of extra bands may be due to the repair mechanism of the irradiation damaged DNA. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that irradiation of parents with 50 and 70 Gy and their F1 are different from the unirradiated pupae It was concluded that the sterile male technique could be used as a benefit tool in controlling Ceratitiis capitata.

Section: Introductionmentioning

confidence: 99%

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Abdalla

Egyptian Academic Journal of Biological Sciences. A, Entomology

et al. 2020

“…The percent of polymorphic bands increased with increases in the EGTA treatment concentration. Ercan [29] and Atienzar et al [52][53][54][55] suggested that the loss of normal bands may be related to the DNA damage, point mutations, or complex chromosomal rearrangements; while appearance of new bands and an increase in band intensity could be ascribed to changes in DNA conformation. The RAPD results from this study suggest that EGTA treatments induced changes to DNA structures.…”

Section: Discussionmentioning

confidence: 99%

“…With the EGTA treatment, polymorphic bands emerged and could be divided into four types: (a) appearance of extra bands; (b) disappearance of normal bands; (c) increased band intensity, and (d) decreased band intensity [29] (Tab. 2 and Tab.…”

Section: Rapd Analysismentioning

confidence: 99%

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

Zhang

Shi

et al. 2016

Acta Soc Bot Pol

In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca 2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca 2+ chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.

“…They showed variations in the band intensity and gain or loss of bands suggesting the probable cause of variation as DNA damage and mutations or structural rearrangements caused by genotoxins, which may have affected the primer sites and/or interpriming distances. Likewise, essential oil of Prangos ferulacea has been reported to induce DNA changes in Ephestia küehniella Zeller larvae (Ercan 2015). Keeping all the above reports and investigations in view, the present work was carried out to explore the larvicidal potential of hexane extracts of leaf and stem of A. aspera and the impact of these extracts on the genomic DNA of early fourth instars of A. aegypti by RAPD-PCR technique.…”

Section: Introductionmentioning

confidence: 95%

Assessment of Achyranthes aspera induced toxicity and molecular analysis of RAPD-PCR profiles of larval genomic DNA of Aedes aegypti L. (Diptera: Culicidae)

2017

Current studies investigated the anti-mosquito potential of against the dengue vector, The stems and leaves of were extracted in hexane and evaluated for their toxicity against early fourth instars of. The larvicidal efficacy of the extract was validated as per WHO protocol. The mortality counts were made after 24 h and LC values were calculated at different levels. The adverse impact of extracts was also explored on the larval genomic DNA. The larvae were exposed to extracts at LC levels and the alterations in -DNA was evaluated through RAPD-PCR technique using three random primers; MA-09, MA-12 and MA-26. Our investigations ascertained the larvicidal efficacy of both the leaf and stem extracts of resulting in respective LC values of 0.068 and 0.082 mg/mL. The extracts also caused variable genotoxic effects with significant changes in the RAPD profiles. The results showed appreciable modifications in larval -DNA with loss of certain bands and gain of unique bands with 82.35% DNA polymorphism. These alterations suggest the probable DNA damage and mutations in the larval-DNA caused by certain phytocomponents which could be the possible reason of larval mortality. Our studies evidenced the anti-mosquito potential of extracts against causing appreciable larval mortality and significant changes in -DNA. The extracts are suggested as efficient and eco-friendly control agent against , yet further investigations are needed to identify the bioactive constituent and ascertain its effectiveness in the field conditions.