Use of Random Amplified Polymorphic DNA PCR To Examine Epidemiology of
 Stenotrophomonas maltophilia
 and
 Achromobacter
 (
 Alcaligenes
 )
 xylosoxidans
 from Patients with Cystic Fibrosis

Krzewinski, Jay; Nguyen, Chau D.; Foster, Jessica M.; Burns, Jane L.

doi:10.1128/jcm.39.10.3597-3602.2001

Cited by 93 publications

(99 citation statements)

References 44 publications

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“…Patient-to-patient transmission between siblings with CF or acquisition of the same strain from an identical environmental source within the family situation has been demonstrated for many bacterial pathogens, including methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Achromobacter xylosoxidans (35,36,37,38). Likewise, concordance in the genotypes colonizing the respiratory tract of siblings suffering from CF has also been reported for some fungal pathogens, like Candida species (39) and Pneumocystis jirovecii (40).…”

Section: Discussionmentioning

confidence: 93%

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

et al. 2016

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The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.R asamsonia argillacea, which was first reported in clinical practice as a Penicillium species, then reclassified in the genus Geosmithia, and finally reassigned in 2013 to the new genus Rasamsonia, is now considered an emerging pathogen (1). Since its first report in humans as Penicillium emersonii (2), there has been an increasing number of cases reported in both dogs (3, 4, 5) and humans (6). Nevertheless, it is likely that the number of human infections caused by this fungus has long been underestimated because of the lack of specificity of its morphological features and to subsequent misidentifications with some Penicillium or Paecilomyces species.Only six species were previously recognized in the genus Rasamsonia described in 2012, i.e., R. argillacea, R. composticola, R. brevistipitata, R. byssochlamydoides, R. cylindrospora, and R. emersonii (7). However, multilocus sequence analysis of several R. argillacea isolates revealed a clustering which superimposed some phenotypic differences. Therefore, R. argillacea is now considered a species complex comprising four distinct species: R. argillacea sensu stricto, R. piperina, R. aegroticola, and R. eburnea (8).Infections caused by species of the R. argillacea complex have been reported in various clinical contexts in human. Chronic granulomatous disease (CGD) (9, 10, 11) and cystic fibrosis (CF) (12,13,14,15,16,17) are the major underlying clinical conditions. Rasamsonia infections may also occur in bone marrow transplant recipients (18, 19) but also may occur in the absence of any predisposing factors, as evidenced by the pulmonary and aortic graft infection reported by Doyon et al. (...

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Section: Discussionmentioning

confidence: 93%

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

et al. 2016

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“…Esse complexo é um grupo taxonômico ainda em crescimento, incluindo, na atualidade, nove diferentes espécies ou variedades genômicas (3,17) . Nos últimos anos, entretanto, outros microrganismos, especialmente gram-negativos não-fermentadores da glicose, vêm sendo apontados como capazes de colonizar a árvore respiratória de portadores de FC (2,11,22) . Neste estudo relata-se a prevalência de espécies bacterianas potencialmente patogênicas encontradas em espécimes respiratórios, escarro e swabs faríngeos de fibrocísticos do Recife.…”

Section: Introductionunclassified

Prevalência de bactérias potencialmente patogênicas em espécimes respiratórios de fibrocísticos do Recife

Magalhães¹,

Britto²,

Bezerra³

et al. 2004

J. Bras. Patol. Med. Lab.

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“…142 In the same multicenter antibiotic trial described above, 92 Achromobacter isolates were analyzed, and 5 instances of shared strains (2 individuals each) were detected. 143 In Athens, Greece, 5 of 9 individuals with CF infected with the same strain of A. xylosoxidans were close social contacts. 196 In a multicenter study conducted in Belgium, 2 clusters of A. xylosoxidans strains were identified (one consisted of 4 individuals, and the other consisted of 10 individuals).…”

Section: Iiia3 Other Gram-negative Bacteriamentioning

confidence: 99%

“…140 Among 183 S. maltophilia isolates obtained from a multicenter antibiotic trial conducted in the United States, only 3 instances of shared strains were found. 143 More recently, 110 isolates recovered from 50 individuals with CF were studied, and 5 distinct strains were identified that were each shared by 2 or 3 patients. 141 Thus, while there is some evidence for shared strains of S. maltophilia and possible patient-to-patient transmission, most infections in people with CF appear to result from independent acquisition, most likely from non-healthcare-associated environmental sources or as a result of antimicrobial selective pressure.…”

Section: Iiia3 Other Gram-negative Bacteriamentioning

confidence: 99%

See 1 more Smart Citation

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

352

382

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The 2013 Infection Prevention and Control (IP&C) Guideline for Cystic Fibrosis (CF) was commissioned by the CF Foundation as an update of the 2003 Infection Control Guideline for CF. During the past decade, new knowledge and new challenges provided the following rationale to develop updated IP&C strategies for this unique population:1. The need to integrate relevant recommendations from evidence-based guidelines published since 2003 into IP&C practices for CF. These included guidelines from the Centers for Disease Control and Prevention (CDC)/Healthcare Infection Control Practices Advisory Committee (HICPAC), the World Health Organization (WHO), and key professional societies, including the Infectious Diseases Society of America (IDSA) and the Society for Healthcare Epidemiology of America (SHEA). During the past decade, new evidence has led to a renewed emphasis on source containment of potential pathogens and the role played by the contaminated healthcare environment in the transmission of infectious agents. Furthermore, an increased understanding of the importance of the application of implementation science, monitoring adherence, and feedback principles has been shown to increase the effectiveness of IP&C guideline recommendations.2. Experience with emerging pathogens in the non-CF population has expanded our understanding of droplet transmission of respiratory pathogens and can inform IP&C strategies for CF. These pathogens include severe acute respiratory syndrome coronavirus and the 2009 influenza A H1N1. Lessons learned about preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant gram-negative pathogens in non-CF patient populations also can inform IP&C strategies for CF.

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Use of Random Amplified Polymorphic DNA PCR To Examine Epidemiology of Stenotrophomonas maltophilia and Achromobacter ( Alcaligenes ) xylosoxidans from Patients with Cystic Fibrosis

Cited by 93 publications

References 44 publications

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Prevalência de bactérias potencialmente patogênicas em espécimes respiratórios de fibrocísticos do Recife

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

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