Use of random amplified polymorphic DNA markers for the detection of Azospirillum strains in soil microcosms

Applicability of the 16S–23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil

“…1998) and ERIC‐PCR (enterobacterial repetitive intergenic consensus‐PCR) as described by Rademaker et al. (1998), and also RAPD analysis using primer 1253 (5′‐GTTTCCGCCC‐3′; Fancelli et al. 1998) as described by Vial et al.…”

“…Research might also focus on a fourth strategy that relies on the identification of strain‐specific DNA sequences and the edition of probes/primers. This approach has been implemented on Azospirillum with randomly selected sequences (Fancelli et al. 1998; Jacoud et al.…”

Evaluating wastewater‐induced plant genotoxicity using randomly amplified polymorphic DNA

“…This technique, developed by Williams et al (1990) and Welsh and McClelland (1990), is a powerful technique that involves the amplification of random segments of genomic DNA using Polymerase Chain Reaction (PCR). Since RAPD is simple, fast, and sensitive, it is now being used in many different fields, such as phylogeny, taxonomy, genotoxicity, and epidemiology (Marillia and Scoles, 1996;Fancelli et al, 1998). The results of RAPD allow the evaluation of how environmental pollutants modify the structure of DNA in living organisms (Conte et al, 1998;Zhiyi and Haowen, 2004).…”

Spontaneous Super-Swarming Derivatives of Azospirillum brasilense Sp245 have Different DNA Profiles and Behavior in the Presence of Various Nitrogen Sources

“…For PCR fingerprinting of the A. brasilense genomes, we used primers specific to repetitive BOX elements [18] and to enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic (REP) sequences [3], as well as primers for random amplified polymorphic DNA (RAPD) analysis [5] (Table 1).…”

“…After 25 cycles, the reaction mixtures were incubated for an additional 5 min at 70°C. The amplification conditions for BOX-, ERIC-, RAPD-, and REP-PCR were as described earlier [3,5,18]. A Tercyc automated thermocycler (DNA-Technology, Russia) was used for DNA amplification.…”