Use of Spheroplast Fusion and Genetic Transformation to Introduce Dextrin Utilization into<i>Saccharomyces Uvarum</i>

Barney, M. C.; Jansen, Gregor; Helbert, J.

doi:10.1094/asbcj-38-0001

Cited by 15 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2016). Although labor- and time-intensive, non-sexual crossing methods such as spheroplast fusion can also be applied (Barney, Jansen and Helbert 1980).…”

Section: Improvement Strategies For Lager-brewing Strainsmentioning

confidence: 99%

Lager-brewing yeasts in the era of modern genetics

Vries

Pronk

Daran

2019

FEMS Yeast Research

View full text Add to dashboard Cite

The yeast Saccharomyces pastorianus is responsible for the annual worldwide production of almost 200 billion liters of lager-type beer. S. pastorianus is a hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus that has been studied for well over a century. Scientific interest in S. pastorianus intensified upon the discovery, in 2011, of its S. eubayanus ancestor. Moreover, advances in whole-genome sequencing and genome editing now enable deeper exploration of the complex hybrid and aneuploid genome architectures of S. pastorianus strains. These developments not only provide novel insights into the emergence and domestication of S. pastorianus but also generate new opportunities for its industrial application. This review paper combines historical, technical and socioeconomic perspectives to analyze the evolutionary origin and genetics of S. pastorianus. In addition, it provides an overview of available methods for industrial strain improvement and an outlook on future industrial application of lager-brewing yeasts. Particular attention is given to the ongoing debate on whether current S. pastorianus originates from a single or multiple hybridization events and to the potential role of genome editing in developing industrial brewing yeast strains.

show abstract

“…2016). Although labor- and time-intensive, non-sexual crossing methods such as spheroplast fusion can also be applied (Barney, Jansen and Helbert 1980).…”

Section: Improvement Strategies For Lager-brewing Strainsmentioning

confidence: 99%

Lager-brewing yeasts in the era of modern genetics

Vries

Pronk

Daran

2019

FEMS Yeast Research

View full text Add to dashboard Cite

show abstract

“…An amylolytic yeast strain would allow the production of a low-calorie beer without the addition of exogenous enzymes to convert dextrins in fermentable sugar. Some attempts have been made by mating (Emeis, 1971) and protoplast fusion (Hockney & Freeman, 1979;Barney et al, 1980), but the more direct approach of the cloning of the arnylolytic gene and its transfer to a brewer's yeast has been more successful. The amyloglucosidase gene of S. diastaticus has been transferred into brewing yeast by Meaden & Tubb (1985), Sakai et al (1988), and Vakeria & Hinchliffe (1989).…”

Section: New Technological Evolutions Raw Materialsmentioning

confidence: 99%