Use of the rice sucrose synthase-1 promoter to direct phloem-specific expression of β-glucuronidase and snowdrop lectin genes in transgenic tobacco plants

She, Ying; Wang, M.B.; Powell, Kevin; Damme, Els J.M. Van; Hilder, Vaughan A.; Gatehouse, Angharad M. R.; Boulter, D.; Gatehouse, John A.

doi:10.1093/jxb/45.5.623

Cited by 101 publications

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“…Therefore the rice sucrosesynthase gene promoter was utilized in the present study to drive the expression of gna in the phloem tissues of rice plants. In earlier studies, this promoter was shown to be phloem specific in transgenic tobacco (Shi et al, 1994), as well as rice (Rao et al, 1998).…”

Section: Discussionmentioning

confidence: 95%

“…The plasmid construct contained the selectable marker gene bar driven by the CaMV 35S promoter (Rathore et al, 1993), and the snowdrop-lectin gene gna driven by phloem-specific rice-sucrose-synthase 1 (RSs1) promoter (Shi et al, 1994). Expression units of bar pG35bar) and gna (RSs1-gna-nos) were cloned at multiple cloning sites of the intermediate vector, pSB11 (Komari et al, 1996), obtained from Japan Tobacco Inc., Japan.…”

Section: Ti Plasmid Construction For Transformation Studiesmentioning

confidence: 99%

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Transgenic indica rice resistant to sap‐sucking insects

Nagadhara

Ramesh

Pasalu

et al. 2003

Plant Biotechnology Journal

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SummaryAgrobacterium-mediated genetic transformation has been optimized in indica rice susceptible to sap-sucking insects, viz., brown planthopper (BPH) and green leafhopper (GLH). Snowdrop lectin gene ( gna) from Galanthus nivalis, driven by phloem-specific rice-sucrose-synthase promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was employed for genetic transformation. Embryogenic calli -after cocultivation with Agrobacterium strain LBA4404 harbouring Ti plasmid pSB111-bar-gnawere selected on the medium containing phosphinothricin. PCR and Southern blot analyses confirmed the stable integration of both the genes into genomes of transgenic (T 0 ) rice plants. Northern and Western blot analyses revealed the expression of gna in the transgenic plants. In the T 1 and T 2 generations, the gna and bar transgenes showed co-segregation at a ratio of 3 : 1. Plant progenies expressing gna, in T 1 and T 2 , exhibited substantial resistance against BPH and GLH pests. This is the first report dealing with transgenic indica rice exhibiting high resistance to both insects.

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Section: Discussionmentioning

confidence: 95%

Section: Ti Plasmid Construction For Transformation Studiesmentioning

confidence: 99%

Transgenic indica rice resistant to sap‐sucking insects

Nagadhara

Ramesh

Pasalu

et al. 2003

Plant Biotechnology Journal

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“…In any case, effects seen with an artificial diet need to be confirmed by assays performed under more or less natural conditions. For testing of hemipteran pests, efforts have been made to target lectin expression specifically to the phloem sap of transgenic plants (Shi et al, 1994). Experiments with biting caterpillars are usually performed with transgenic lines that show high level constitutive expression of the lectin (Fitches et al, 1997).…”

Section: Insectsmentioning

confidence: 99%

“…GNA is one of the most closely studied plant lectins, mainly because it was the first plant lectin shown to be active against Hemiptera and the protein is easy to purify (Shi et al, 1994;Hilder et al, 1995). It specifically binds to high-mannose glycans, in particular to terminal mannose residues.…”

Section: Gna: a Mannose-specific Lectin With Negative Effects On Sevementioning

confidence: 99%

Plant‐insect interactions: what can we learn from plant lectins?

Michiels

Damme

Smagghe

2010

Arch Insect Biochem Physiol

120

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Many plant lectins have high anti-insect potential. Although the effects of most lectins are only moderately influencing development or population growth of the insect, some lectins have strong insecticidal properties. In addition, some studies report a deterrent activity towards feeding and oviposition behavior. Transmission of plant lectins to the next trophic level has been investigated for several tritrophic interactions. Effects of lectins with different sugar specificities can vary substantially with the insect species under investigation and with the experimental setup. Lectin binding in the insect is an essential step in exerting a toxic effect. Attempts have been made to study the interactions of lectins in several insect tissues and to identify lectin-binding receptors. Ingested lectins generally bind to parts of the insect gut. Furthermore, some lectins such as the Galanthus nivalus agglutinin (GNA) cross the gut epithelium into the hemolymph and other tissues. Recently, several candidate lectinbinding receptors have been isolated from midgut extracts. To date little is known about the exact mechanism for insecticidal activity of plant lectins. However, insect glycobiology is an emerging research field and the recent Grant sponsor: Fund for Scientific Research-Flanders, Brussels; Grant number: 3G016306.Correspondence to: Guy Smagghe, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. E-mail: guy.smagghe@ugent.be Vol. 73, No. 4, 193-212 (2010 ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,

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“…For phloem-specific expression of DB1, the rice sucrose synthase-1 (RSs1) promoter (accession no. AJ401233) was employed as described (Shi et al 1994). The promoter sequence (3 kb) was amplified from rice cv.…”

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confidence: 99%

Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid

Kato

Hori

Ogawa

et al. 2010

Plant Biotechnology

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Dioscorea batatas tuber lectin 1 (DB1) is a storage protein isolated from yam tuber, and is shown to be a mannose-binding lectin. It has 58% amino-acid identity to insecticidal snowdrop bulb lectin GNA. In this study, we demonstrated that Ն1 mg ml Ϫ1 DB1 in an artificial diet significantly decreased the survival and fecundity of green peach aphid, Myzus persicaeca. We produced transgenic tobacco plants expressing cDNA of DB1 under the control of Cauliflower mosaic virus 35S promoter (35S-DB1) or phloem-specific promoter of rice sucrose synthase-1 gene (RSs1-DB1), and evaluate the degree of aphid resistance in whole plant bioassays. The number of survival aphids was reduced to 60% in transgenic lines with 35S-DB1 and RSs1-DB1, which accumulated DB1 at a level of 1.8% and 0.25%, respectively, of total soluble protein. Our results indicate that DB1 can be used to enhance resistance to sap-sucking insects in transgenic crops.Key words: Aphid, Dioscorea batatas tuber lectin 1, insect resistance, transgenic tobacco.Plant Biotechnology 27, 141-145 (2010) Original PaperThis article can be found at http://www.jspcmb.jp/ Materials and methods Artificial diet bioassaysGreen peach aphid (Myzus persicae) was obtained form JT (Japan Tobacco Inc. Japan). Aphids were maintained on mature plants of Nicotiana tabacum cv. Petit Havana SR1 in a controlled environment growth chamber, 25 Ϯ 1 o C, 16L/8D. The artificial diet bioassays for aphids was carried out as described by Powell et al. (1993) using an artificial diet for aphids (Dadd and Mitter 1966). DB1 was incorporated at 2, 1 and 0.1 mg ml Ϫ1 in the artificial diet. Controls were set as treatment with feed using an artificial diet containing 0 mg ml Ϫ1 DB1 or 1 mg ml Ϫ1 BSA, and treatment with no diet (no diet controls). Ten adult apterous aphids were transferred from the host plant to glass petri-dishes. Each petri-dish was sealed with a stretched Parafilm (Pechiney Plastic Packaging Inc., USA), an 500 ml artificial diet was placed on top of the stretched Parafilm, and then the artificial diet was covered with another stretched Parafilm. The diet and Parafilm were changed daily. The bioassays were conducted in an environmental growth chamber (25 Ϯ 1°C, 16L/8D), and the number of surviving adults and newborn nymphs was counted with four sets of replication. A statistical analysis was carried out using the Excel Statistics software. Transformation of tobaccoThe nucleotide sequence for a DB1 isoform, DB1 (Leu86), under the accession no. AB178475 in DDBJ was revealed to be lacking a part of 5Ј signal sequence. PCR for 5Ј RACE was carried out to obtain full length cDNA using yam tuber mRNA (Ohizumi et al. 2009) and the Marathon cDNA Amplification Kit (BD Bioscience Clontech). The nucleotide sequence of full length open reading frame (ORF) of DB1 cDNA was deposited to DDBJ (accession no. AB513659). It encodes 172 aminoacid protein with a predicted targeting-signal sequence to endoplasmic reticulum.The cDNA covering full-length ORF was PCR cloned into pGEM T-vectors (P...

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Use of the rice sucrose synthase-1 promoter to direct phloem-specific expression of β-glucuronidase and snowdrop lectin genes in transgenic tobacco plants

Cited by 101 publications

References 0 publications

Transgenic indica rice resistant to sap‐sucking insects

Transgenic indica rice resistant to sap‐sucking insects

Plant‐insect interactions: what can we learn from plant lectins?

Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid

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