Use of virulence factor-specific egg yolk-derived immunoglobulins as a promising alternative to antibiotics for prevention of attaching and effacing<i>Escherichia coli</i>infections

Girard, Francis; Batisson, Isabelle; Martínez, Gabriela; Breton, Cynthia; Harel, J; Fairbrother, John M.

doi:10.1111/j.1574-695x.2005.00030.x

Cited by 36 publications

(31 citation statements)

References 45 publications

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“…Oral administration of anti-intimin IgY antibodies has recently been shown to be a promising strategy for prophylaxis of attaching and effacing Escherichia coli (AEEC) infections in weaned pigs (Girard et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

et al. 2007

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IgY is a chicken egg yolk antibody which has been used for treatment and prophylaxis of gastrointestinal infections. Our aim was to verify if IgY obtained from chickens immunized with EPEC O111, STEC O111 and STEC O157 is able to show in vitro reactivity and biological activity towards the three bacteria. IgY was obtained from eggs laid before and after immunization with each bacterium. The preparations of IgY anti-EPEC O111 and anti-STEC O111 shared high reactivity detected by ELISA and growth inhibition ability towards both bacteria EPEC O111 and STEC O111. Nevertheless, the preparation of IgY anti-STEC O157 showed high reactivity and growth inhibitory effect only towards the homologous strain. Our results showing in vitro biological activity of IgY reinforce its use as an alternative for the treatment or prophylaxis of E. coli infections and encourage the development of in vivo studies for a possible future human therapeutic use.

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Section: Introductionmentioning

confidence: 99%

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

et al. 2007

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“…또한 난황항체는 대장균 설사증 (Yokoyama et al, 1992;Marquardt et al, 1999;김 등, 2000;Fulton et al, 2002;Girard et al, 2006) …”

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Effects of Egg Yolk Antibody Powder (IgY) Supplementation on Growth Performance, Blood Component Profile, Intestinal Microflora, and Immunoglobulin G in Meat Ducks

Jeon¹,

Kang²,

Kim³

et al. 2016

Korean Journal of Poultry Science

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This study was conducted to investigate the effect of dietary egg yolk antibody (IgY) powder supplementation on the growth performance, blood component profile, intestinal microflora, and immunoglobulin G in ducks. A total of 300 1-day-old ducks (Cherry Valley) were randomly divided into 5 groups, with 3 replicates of 20 birds. The treatment groups were the negative (NC), positive (PC), egg yolk antibody powder 0.1% (T1), egg yolk antibody powder 0.5% (T2), and egg yolk antibody powder 1.0% (T3) groups. In the growth performance of ducks during the entire experimental period, the IgY groups and positive control group (PC) showed significantly higher (P<0.05) body weights and body weight gains compared to the negative control group (NC). However, no significant differences were observed in the feed intake and feed conversion ratio. The blood component profiles showed that the IgY 0.1 and 1.0% groups decreased in total cholesterol content compared to the NC group. The aspartate aminotransferase (AST) and alanine aminotranferease (ALT) contents were lower in the IgY 1.0% group, but there were no significant differences. Regrading the leukocyte content after feeding IgY, the heterophil: lymphocyte ratio decreased in the IgY groups, especially in the IgY 0.1% group, which had a lower content than the other groups. However, these results showed no significant differences. The Lactobacillus count in the intestines significantly increased (P<0.05) in the IgY 0.1 and 0.5% groups, the level of IgY increased, and the Escherichia coli count decreased. However, no significant difference was observed in the total plate count. The immunoglobulin G content was lower in the IgY groups than in the NC group, and compared with the IgY groups, the IgY 0.5% had a lower content, which was not a significant difference

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“…13,[22][23][24][25][26] In the present study, we immunized chickens with rStx-1B and rStx-2B. The antibody titer increased at 2-4 weeks after booster immunization, and antibody production continued for a further 7-8 weeks.…”

Section: Discussionmentioning

confidence: 78%

Recombinant Shiga Toxin B Subunit Can Induce Neutralizing Immunoglobulin Y Antibody

Neri

Tokoro

Sugiyama

et al. 2012

Biological & Pharmaceutical Bulletin

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Previously, we have shown that chickens immunized with Shiga toxin (Stx) produce Stx-neutralizing egg yolk immunoglobulin Y (IgY) antibody. The anti-Stx-1 IgY and anti-Stx-2 IgY exert their neutralizing activity through their antibody activity against the B subunit of the toxin but not the A subunit. In the present study, chickens were immunized with recombinant Stx-1 B subunit (rStx-1B) and recombinant Stx-2 B subunit (rStx-2B). Induced anti-rStx-1B and anti-rStx-2B IgY neutralized the toxicity of Stx-1 and Stx-2 against HeLa 229 cells. The neutralizing activity of anti-rStx-1B IgY on Stx-1 was almost 10 times stronger than that of anti-Stx-1 IgY, and that of anti-rStx-2B IgY was 2.6 times stronger than that of anti-Stx-2 IgY. Anti-rStx-1B and anti-rStx-2B IgY reacted with multimeric and monomeric forms of the B subunits in contrast to anti-Stx-1 and anti-Stx-2 IgY that reacted with only the multimeric form. These results indicated that recombinant B subunits were promising antigens for induction of neutralizing antibodies in chickens.

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Use of virulence factor-specific egg yolk-derived immunoglobulins as a promising alternative to antibiotics for prevention of attaching and effacingEscherichia coliinfections

Cited by 36 publications

References 45 publications

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

Effects of Egg Yolk Antibody Powder (IgY) Supplementation on Growth Performance, Blood Component Profile, Intestinal Microflora, and Immunoglobulin G in Meat Ducks

Recombinant Shiga Toxin B Subunit Can Induce Neutralizing Immunoglobulin Y Antibody

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