Using a Modified Ferrous Oxidation−Xylenol Orange (FOX) Assay for Detection of Lipid Hydroperoxides in Plant Tissue

DeLong, John M.; Prange, Robert K.; Hodges, D. Mark; Forney, Charles F.; Bishop, Marie-France; Quilliam, Michael A.

doi:10.1021/jf0106695

Cited by 152 publications

(123 citation statements)

References 60 publications

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“…On the other hand, they are criticized for their non-specificity [42,43]. Lipid hydroperoxides may be determined by the iodometric method and FOX test [44].…”

Section: Spectrophotometric Methods In Lipid Peroxidation Analysismentioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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“…On the other hand, they are criticized for their non-specificity [42,43]. Lipid hydroperoxides may be determined by the iodometric method and FOX test [44].…”

Section: Spectrophotometric Methods In Lipid Peroxidation Analysismentioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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“…The extent of lipid peroxidation was estimated by measuring the levels of lipid hydroperoxides in leaves. Lipid hydroperoxide levels were estimated spectrophotometrically following a modified ferrous oxidation-xylenol orange (FOX) assay, as described in DeLong et al (2002).…”

Section: Leaf Water Contents Pigment Levels and Lipid Peroxidationmentioning

confidence: 99%

Adaptation of the Long-Lived Monocarpic Perennial, Saxifraga longifolia to High Altitude

et al. 2016

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Global change is exerting a major effect on plant communities, altering their potential capacity for adaptation. Here, we aimed at unveiling mechanisms of adaptation to high altitude in an endemic long-lived monocarpic, Saxifraga longifolia, by combining demographic and physiological approaches. Plants from three altitudes (570, 1100, and 2100 m above sea level [a.s.l.]) were investigated in terms of leaf water and pigment contents, and activation of stress defense mechanisms. The influence of plant size on physiological performance and mortality was also investigated. Levels of photoprotective molecules (a-tocopherol, carotenoids, and anthocyanins) increased in response to high altitude (1100 relative to 570 m a.s.l.), which was paralleled by reduced soil and leaf water contents and increased ABA levels. The more demanding effect of high altitude on photoprotection was, however, partly abolished at very high altitudes (2100 m a.s.l.) due to improved soil water contents, with the exception of a-tocopherol accumulation. a-Tocopherol levels increased progressively at increasing altitudes, which paralleled with reductions in lipid peroxidation, thus suggesting plants from the highest altitude effectively withstood high light stress. Furthermore, mortality of juveniles was highest at the intermediate population, suggesting that drought stress was the main environmental driver of mortality of juveniles in this rocky plant species. Population structure and vital rates in the high population evidenced lower recruitment and mortality in juveniles, activation of clonal growth, and absence of plant sizedependent mortality. We conclude that, despite S. longifolia has evolved complex mechanisms of adaptation to altitude at the cellular, whole-plant and population levels, drought events may drive increased mortality in the framework of global change.

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“…Lipid peroxide content was measured using the ferrous oxidation xylenol orange assay as described previously (DeLong et al, 2002;Sattler et al, 2004) with the following modifications. Leaf samples (25 to 30 mg) harvested at the end of the light cycle were immediately extracted with 200 mL of methanol containing 0.01% (w/v) butylated hydroxytoluene, 200 mL of dichloromethane, and 50 mL of 150 mM acetic acid using three 3-mm glass beads and a commercial paint shaker.…”

Section: Lipid Peroxide Analysismentioning

confidence: 99%

“…The triphenyl phosphine-treated and untreated samples (100 mL) were incubated with 900 mL of ferrous oxidation xylenol orange reagent (90% [v/v] methanol, 4 mM butylated hydroxytoluene, 25 mM sulfuric acid, 250 mM ferrous ammonium sulfate, and 100 mM xylenol orange) at room temperature for exactly 20 min, and A 560 was measured. Lipid peroxide content was calculated based on a standard curve of hydrogen peroxide, as described previously (DeLong et al, 2002).…”

Section: Lipid Peroxide Analysismentioning

confidence: 99%

Tocopherols Play a Crucial Role in Low-Temperature Adaptation and Phloem Loading in Arabidopsis

et al. 2006

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To test whether tocopherols (vitamin E) are essential in the protection against oxidative stress in plants, a series of Arabidopsis thaliana vitamin E (vte) biosynthetic mutants that accumulate different types and levels of tocopherols and pathway intermediates were analyzed under abiotic stress. Surprisingly subtle differences were observed between the tocopheroldeficient vte2 mutant and the wild type during high-light, salinity, and drought stresses. However, vte2, and to a lesser extent vte1, exhibited dramatic phenotypes under low temperature (i.e., increased anthocyanin levels and reduced growth and seed production). That these changes were independent of light level and occurred in the absence of photoinhibition or lipid peroxidation suggests that the mechanisms involved are independent of tocopherol functions in photoprotection. Compared with the wild type, vte1 and vte2 had reduced rates of photoassimilate export as early as 6 h into low-temperature treatment, increased soluble sugar levels by 60 h, and increased starch and reduced photosynthetic electron transport rate by 14 d. The rapid reduction in photoassimilate export in vte2 coincides with callose deposition exclusively in phloem parenchyma transfer cell walls adjacent to the companion cell/sieve element complex. Together, these results indicate that tocopherols have a more limited role in photoprotection than previously assumed but play crucial roles in low-temperature adaptation and phloem loading.

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Using a Modified Ferrous Oxidation−Xylenol Orange (FOX) Assay for Detection of Lipid Hydroperoxides in Plant Tissue

Cited by 152 publications

References 60 publications

Automation of Methods for Determination of Lipid Peroxidation

Automation of Methods for Determination of Lipid Peroxidation

Adaptation of the Long-Lived Monocarpic Perennial, Saxifraga longifolia to High Altitude

Tocopherols Play a Crucial Role in Low-Temperature Adaptation and Phloem Loading in Arabidopsis

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