Using Capillary Electrophoresis to Determine the Purity of Acetylsalicylic Acid Synthesized in the Undergraduate Laboratory

Welder, Frank; Colyer, Christa L.

doi:10.1021/ed078p1525

Cited by 11 publications

(8 citation statements)

References 11 publications

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“…A number of these experiments have focused on small ion separations (5,6,8,18,22). Only one experiment was explicitly designed for the first-or second-year course sequence (23).…”

Section: Experimental Considerationsmentioning

confidence: 99%

Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory

2004

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Capillary electrophoresis is gradually working its way into the undergraduate laboratory curriculum. Typically, experiments utilizing this newer technology have been introduced into analytical or instrumental courses. We have introduced an experiment into the introductory laboratory that utilizes capillary electrophoresis to identify and quantitate cations in common water samples. This experiment can be done in a lab, as a "dry-lab", or as an in-class exercise. Students explore a number of basic separation topics and work with figures of merit for real and meaningful samples. We have adopted a strategy where students encounter various instruments and methods of analyses multiple times throughout the curriculum. The level of sophistication in the exposures increases with the experience level of the students. Through this repeated exposure students will gain a better and fuller understanding of these methods than they would with a single exposure to the technique in the analytical or instrumental laboratory. This is the first of at least four experiments that our chemistry and biochemistry students encounter involving capillary electrophoresis methods.

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“…A number of these experiments have focused on small ion separations (5,6,8,18,22). Only one experiment was explicitly designed for the first-or second-year course sequence (23).…”

Section: Experimental Considerationsmentioning

confidence: 99%

Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory

2004

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“…A number of undergraduate laboratory experiments utilizing CE have been developed subsequent to the availability of CE instrumentation analyzing various small molecules, − inorganic ions, − and biopolymers. ,− While effective in demonstrating the use of CE across a wide range of compounds, these experiments utilize UV absorbance almost exclusively as a means of detection while neglecting to utilize other detection methods. To our knowledge, an undergraduate laboratory experiment using CE-LIF has yet to be published.…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Diagnosis of Phenylketonuria: A Versatile Wet or Dry Laboratory Experiment in Upper-Level Undergraduate Analytical Chemistry

2021

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Capillary electrophoresis is a powerful separation tool with a wide range of analytes for diverse applications and is compatible with various detection methods. While some more established separation techniques like gas or liquid chromatography are utilized heavily in analytical chemistry laboratory courses, newer commercial capillary electrophoresis instrumentation remains underrepresented in undergraduate laboratories, with the concepts being largely in the realm of lecture alone in analytical chemistry curricula. Despite its proven power and wide application, an undergraduate laboratory experiment utilizing capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) has yet to be published. Here, we present an experiment for third-and fourthyear undergraduate analytical chemistry courses in which the students use CE-LIF to optimize system parameters, analyze the amino acid profile of a mock "patient sample", and use calibration standards coupled with statistical analysis to provide a phenylketonuria (PKU) diagnosis. This experiment is easily adaptable to a dry lab, making this technique available to laboratories without the resources needed to acquire a CE-LIF system. We also demonstrate that the dry lab analysis can be accomplished in an online format, requiring no student presence in the laboratory, which is especially relevant due to recent events concerning the COVID-19 pandemic.

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“…Indeed, CE is routinely used in research applications for the analysis of peptides. − Microfluidic CE devices are often connected to electrospray mass spectrometers for proteomic analysis. − Capillary electrophoresis is covered in most analytical textbooks, and several laboratory experiments have been developed to demonstrate the power of this technique . These include laboratories aimed at determining the amount of caffeine in various foods and beverages, − separation of barbiturates, analysis of cations in soil and water samples, − studying the reaction products from an organic chemistry experiment, , the analysis of various foods and food dyes, and analgesic formulations . Laboratories with biological applications include determining protein amino acid composition, determining the amino acid composition of a dipeptide using the related micellar electrokinetic capillary chromatography technique, carbohydrate analysis, and determining the diffusion constant of dopamine .…”

Section: Introductionmentioning

confidence: 99%

Capillary Zone Electrophoresis for the Analysis of Peptides: Fostering Students’ Problem-Solving and Discovery Learning in an Undergraduate Laboratory Experiment

Albright

Beussman

2017

J. Chem. Educ.

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Capillary electrophoresis is an important analytical separation method used to study a wide variety of samples, including those of biological origin. Capillary electrophoresis may be covered in the classroom, especially in advanced analytical courses, and while many students are exposed to gel electrophoresis in biology or biochemistry laboratories, capillary electrophoresis is not as commonly found in undergraduate lab curricula. Published lab experiments that incorporate capillary electrophoresis include the analysis of caffeine in beverages and cations in water samples but not of more biological samples such as a peptide mixture. This paper introduces a peptide separation based on capillary zone electrophoresis that is both qualitative and quantitative. It describes the separation of four small peptides where two of the analytes have similar migration times, forcing students to think about how they can best identify which peak corresponds to which analyte, while exposing them to a bioanalytical application of capillary electrophoresis.

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Using Capillary Electrophoresis to Determine the Purity of Acetylsalicylic Acid Synthesized in the Undergraduate Laboratory

Cited by 11 publications

References 11 publications

Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory

Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory

Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Diagnosis of Phenylketonuria: A Versatile Wet or Dry Laboratory Experiment in Upper-Level Undergraduate Analytical Chemistry

Capillary Zone Electrophoresis for the Analysis of Peptides: Fostering Students’ Problem-Solving and Discovery Learning in an Undergraduate Laboratory Experiment

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