Using Collision Cross Section Distributions to Assess the Distribution of Collision Cross Section Values

Abstract

In this study we explore the use of collision cross section distributions to allow comparability of IM-MS data for proteins on different instruments. We present measurements on seven standard proteins across three IM-MS configurations, namely an Agilent 6560 IM QToF, a Waters Synapt G2 possessing a TWIMS cell and a modified Synapt G2 possessing an RF confining linear field drift cell. Mobility measurements were taken using both He and N<sub>2</sub> as the drift gases. To aid comparability across instruments and best assess the corresponding gas-phase conformational landscapes of the protein ‘standards’ we present the data in the form of averaged collision cross section distributions.