Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis

Chen, Min; Bui, Anhthu Q.; Goldberg, Robert B.

doi:10.1007/978-1-0716-0342-0_15

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…We used SRB embryo medial sections to avoid potential contamination from surrounding seed tissue during embryo proper and suspensor region LCM ( Fig. 1K) (see Materials and Methods) (20,33). We searched our embryo proper and suspensor RNA-Seq reads for endothelium-specific transcripts (G563) (SI Appendix Fig.…”

Section: Absence Of Contamination From Surrounding Seed Tissue In Embmentioning

confidence: 99%

“…The large size of SRB and CB embryos permits hand dissection of embryo proper and suspensor regions for experimental studies (Appendix SI Fig. S1A), which is not practical in SB and Arabidopsis (20). Almost five decades ago Ian Sussex, Mary Clutter, Ed Yeung, and their colleagues took advantage of this property to show that giant SRB and CB suspensors are highly active and supply the embryo proper with substances responsible for embryo proper growth (21)(22)(23) -sustaining the ideas of pioneering plant embryologists of the late 19th and early 20th centuries (8,9).…”

Section: Introductionmentioning

confidence: 99%

“…Pods were harvested 5-7 days after pollination (DAP) to obtain globular stage embryos from seeds 2.0 to 2.5 mm in length(Fig. 1A-C).CB.Phaseolus vulgaris (Andean inbred landrace accession G19833) seeds were planted and CB plants were grown under the same conditions as SRB(20), except after one month they were moved into a growth chamber with an 8 h/16 h light/dark cycle for one week to induce flowering.…”

mentioning

confidence: 99%

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Comparative Analysis of Embryo Proper and Suspensor Transcriptomes in Plant Embryos With Different Morphologies

Chen¹,

Lin²,

Wu³

et al. 2020

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An important question is what genes govern the differentiation of plant embryos into suspensor and embryo-proper regions following fertilization and division of the zygote. We compared embryo proper and suspensor transcriptomes of four plants that vary in embryo morphology within the suspensor region. We determined that genes encoding enzymes in several metabolic pathways leading to the formation of hormones, such as gibberellic acid, and other metabolites are up-regulated in giant Scarlet Runner Bean and Common Bean suspensors. Genes involved in transport and Golgi body organization are up-regulated within the suspensors of these plants as well - strengthening the view that giant specialized suspensors serve as a hormone factory and a conduit for transferring substances to the developing embryo proper. By contrast, genes controlling transcriptional regulation, development, and cell division are up-regulated primarily within the embryo proper. Transcriptomes from less specialized soybean and Arabidopsis suspensors demonstrated that fewer genes encoding metabolic enzymes and hormones are up-regulated. Genes active in the embryo proper, however, are functionally similar to those active in Scarlet Runner Bean and Common Bean embryo proper regions. We uncovered a set of suspensor- and embryo-proper-specific transcription factors (TFs) that are shared by all embryos irrespective of morphology, suggesting that they are involved in early differentiation processes common to all plants. ChIP-Seq experiments with Scarlet Runner Bean and soybean WOX9, an up-regulated suspensor TF, gained entry into a regulatory network important for suspensor development irrespective of morphology.

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Section: Absence Of Contamination From Surrounding Seed Tissue In Embmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Comparative Analysis of Embryo Proper and Suspensor Transcriptomes in Plant Embryos With Different Morphologies

Chen¹,

Lin²,

Wu³

et al. 2020

Preprint

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“…G12882 was grown at 28/24°C under an 11 h light/13 h dark cycle. Phaseolus coccineus was hand pollinated according to Chen et al (2020). Designation of developmental stages of the seed is according to Walbot et al (1972).…”

Section: Methodsmentioning

confidence: 99%

Pectin acetylesterase 8 influences pectin acetylation in the seed coat, seed imbibition, and dormancy in common bean (Phaseolus vulgaris L.)

et al. 2021

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The results of a prior transcript profiling study identified a large difference in transcript accumulation of a pectin acetylesterase gene, designated as PAE8, in two genetically related lines of common bean (Phaseolus vulgaris). The results of reverse transcription‐quantitative PCR experiments confirmed this difference and revealed that the gene is expressed specifically in the seed coat of developing seeds. Genomic sequence data identified a non‐functional allele, due to a five‐base pair insertion resulting in a frameshift and premature stop codon. The non‐functional pae8 allele was associated with a lack of detectable protein as determined by Western blot. PAE8 accounted for approximately 65% of total pectin acetylesterase activity in the developing seed coat. Lack of functional PAE8 resulted in an approximately 2.5‐fold increase in acetylation of soluble pectin in the mature seed coat. The presence of a non‐functional pae8 allele was associated with an increased rate of water absorption by the seed and increased percentage of germination in aged seeds. The data suggest that the decreased acetylation of pectin leads to enhanced interaction with Ca2+, contributing to water impermeability.

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Protocorm Regeneration and Protocorm-Like Bodies

Yeung,

Stasolla

2024

Springer Protocols Handbooks

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Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis

Cited by 6 publications

References 26 publications

Comparative Analysis of Embryo Proper and Suspensor Transcriptomes in Plant Embryos With Different Morphologies

Comparative Analysis of Embryo Proper and Suspensor Transcriptomes in Plant Embryos With Different Morphologies

Pectin acetylesterase 8 influences pectin acetylation in the seed coat, seed imbibition, and dormancy in common bean (Phaseolus vulgaris L.)

Protocorm Regeneration and Protocorm-Like Bodies

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