Using gold nanostars modified pencil graphite electrode as a novel substrate for design a sensitive and selective Dopamine aptasensor

Talemi, Rasoul Pourtaghavi; Mousavi, Seyed Mehdi; Afruzi, Hossein

doi:10.1016/j.msec.2016.12.119

Cited by 26 publications

(14 citation statements)

References 38 publications

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“…An electrode modified with gold nanostars and a dopaminebinding aptamer exhibited a very low detection limit of 0.0019 nM. The presence of many interferents did not hinder the sensitivity of the biosensor due to the specific binding between the aptamers and the dopamine molecules [68]. Similarly, a platform composed of a spindle-shaped gold nanostructure and dopamine-binding aptamers showed high selectivity, high stability, and a limit detection of 0.01 nM using differential pulse voltammetry [69].…”

Section: General Overview Of Modification Materials Formentioning

confidence: 99%

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

2021

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Neurotransmitters are biochemical molecules that transmit a signal from a neuron across the synapse to a target cell, thus being essential to the function of the central and peripheral nervous system. Dopamine is one of the most important catecholamine neurotransmitters since it is involved in many functions of the human central nervous system, including motor control, reward, or reinforcement. It is of utmost importance to quantify the amount of dopamine since abnormal levels can cause a variety of medical and behavioral problems. For instance, Parkinson’s disease is partially caused by the death of dopamine-secreting neurons. To date, various methods have been developed to measure dopamine levels, and electrochemical biosensing seems to be the most viable due to its robustness, selectivity, sensitivity, and the possibility to achieve real-time measurements. Even if the electrochemical detection is not facile due to the presence of electroactive interfering species with similar redox potentials in real biological samples, numerous strategies have been employed to resolve this issue. The objective of this paper is to review the materials (metals and metal oxides, carbon materials, polymers) that are frequently used for the electrochemical biosensing of dopamine and point out their respective advantages and drawbacks. Different types of dopamine biosensors, including (micro)electrodes, biosensing platforms, or field-effect transistors, are also described.

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Section: General Overview Of Modification Materials Formentioning

confidence: 99%

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

2021

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“…This sensor was successfully applied to spiked serum samples showing good recoveries. Talemi et al designed a sensitive and selective dopamine aptasensor based on gold nanostar‐modified pencil graphite as a novel substrate 120 . The thiolated DNA aptamer for dopamine was immobilized on the gold nanostars coated with positively charged cetyltrimethylammonium bromide (CTAB) surfactant.…”

Section: Electochemical Biosensors Based On Oxidation Of Dopaminementioning

confidence: 99%

Biosensors and sensors for dopamine detection

Liu

2020

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196

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Dopamine is a key catecholamine neurotransmitter and it has critical roles in the function of the human central nervous system. Abnormal release of dopamine is related to neurological diseases and depression. Therefore, it is necessary to monitor dopamine levels in vivo and in real time to understand its physiological roles. In this review, we discuss dopamine detection focusing on the molecular recognition methods including enzymes, antibodies, and aptamers, as well as new advances based on nanomaterials and molecularly imprinted polymers (MIPs). A large fraction of these sensors rely on electrochemical detection to fulfill the requirement of fast, in situ, and in vivo detection with a high spatial and temporal resolution. These methods need to overcome interferences from molecules with a similar redox potential. In addition, fluorescent and colorimetric sensors based on aptamers are also quite popular, and care needs to be taken to validate specific dopamine binding. Combining aptamers or MIPs with electrochemistry promises to achieve rapid detection and increased selectivity. In this article, we pay more attention to the molecular recognition mechanism and critically review the sensor designs. In the end, some future directions are discussed.

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“…Multiple sensors for the detection of dopamine have been developed using either the RNA or DNA DBA. − In one instance, both the DNA and RNA aptamers were investigated in the same platform and the DNA homologue aptamer showed increased sensitivity and specificity . Since the binding affinity and selectivity of the DBA has been well characterized by several independent research groups, the DBA was investigated as the main aptamer of interest in this work and the novel aptamer selected in this work was used to validate the generality of the system.…”

Section: Resultsmentioning

confidence: 99%

In Vivo Use of a Multi-DNA Aptamer-Based Payload/Targeting System To Study Dopamine Dysregulation in the Central Nervous System

McConnell

Ventura

Dwyer

et al. 2018

ACS Chem. Neurosci.

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The delivery of therapeutics across the blood-brain barrier remains a considerable challenge in investigating central nervous system related processes. In this work, a liposome vehicle was surface-modified with an aptamer that binds to the transferrin receptor and was loaded with two different dopamine-binding aptamer payloads. This system was effectively used to promote the delivery of the aptamer cargo from the peripheral injection site into the brain. The effect of these delivered aptamers on behavior was investigated in vivo in a locomotor task. The first dopamine binding aptamer assessed was a DNA aptamer, the binding of which had been previously validated through the aptamer-based biosensor development reported by several independent research groups. The second aptamer investigated was the result of a novel in vitro selection experiment described herein. Our data suggest that systemic administration of the modified liposomes led to delivery of the dopamine aptamers into the brain. Fluorescence microscopy revealed differential distribution of fluorescence based on the presence or absence of the transferrin receptor aptamer on the surface of fluorescently modified liposomes. In a behavioral experiment using cocaine administration to induce elevated concentrations of neural dopamine, systemic pretreatment with the dopamine aptamer-loaded liposomes reduced cocaine-induced hyperlocomotion. Multiple controls including a transferrin-negative liposome control and transferrin-positive liposomes loaded with either a nonbinding, base-substituted dopamine aptamer or a random oligonucleotide were investigated. None of these controls altered cocaine-induced hyperlocomotion. Chronic systemic administration of the modified liposomes produced no deleterious neurobehavioral or neural degenerative effects. Importantly, this work is one example of an application for this versatile multiaptamer payload/targeting system. Its general application is limited only by the availability of aptamers for specific neural targets.

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Using gold nanostars modified pencil graphite electrode as a novel substrate for design a sensitive and selective Dopamine aptasensor

Cited by 26 publications

References 38 publications

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

Biosensors and sensors for dopamine detection

In Vivo Use of a Multi-DNA Aptamer-Based Payload/Targeting System To Study Dopamine Dysregulation in the Central Nervous System

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