Using HPLC with mass-spectrometric detection for riboflavin determination in complex medicinal forms

Mitrofanov, D. A.; Nazarov, G. V.; Babkin, I. Yu.; Галан, С. Е.; Goncharov, V. M.; Вострухов, С. В.

doi:10.1007/s11094-009-0258-3

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…Nowadays, high-performance liquid chromatography (HPLC) is one of the most reliable analytical techniques for separating, quantifying, and identifying fluorochromes [ 21 , 22 , 23 ]. Likewise, nuclear magnetic resonance spectroscopy and mass spectrometry are powerful analytical tools that have been used to quantify known fluorochromes, as well as to elucidate the chemical identity, structure, and properties of fluorochromes [ 24 , 25 , 26 ].…”

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confidence: 99%

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

Jiménez-Nava,

Zepeda-Vallejo,

Santoyo-Tepole

et al. 2023

Biomolecules

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The yeast Hyphopichia wangnamkhiaoensis excretes a brilliant yellow fluorescent compound into its growth culture. In this study, we isolated and identified this compound using reverse-phase high-performance liquid chromatography-diode array detector (RP-HPLC-DAD) as well as 1H NMR and UV–Vis spectroscopy. Two of the three RP-HPLC-DAD methods used successfully separated the fluorescent compound and involved (1) a double separation step with isocratic flow elution, first on a C18 column and later on a cyano column, and (2) a separation with a linear gradient elution on a phenyl column. The wavelengths of maximum absorption of the fluorescent compound-containing HPLC fractions (~224, 268, 372, and 446 nm) are in good agreement with those exhibited by flavins. The 1H NMR spectra revealed methyl (δ 2.30 and 2.40) and aromatic proton (δ 7.79 and 7.77) signals of riboflavin. The 1H NMR spectra of the samples spiked with riboflavin confirmed that the brilliant yellow fluorescent compound is riboflavin. The maximum excitation and emission wavelengths of the fluorescent compound were 448 and 528 nm, respectively, which are identical to those of riboflavin.

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Section: Introductionmentioning

confidence: 99%

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

Jiménez-Nava,

Zepeda-Vallejo,

Santoyo-Tepole

et al. 2023

Biomolecules

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A Novel Direct Method for Determination of Riboflavin in Alcoholic Fermented Beverages

et al. 2015

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N,S Co-Doped Graphene Quantum Dots for Detection of Riboflavin and Cell Imaging

Zhang

Xiao

et al. 2021

NANO

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Graphene quantum dots (GQDs) have been extensively used in biosensors and bioimaging. Heteroatom-doped GQDs can regulate material properties and endow them to improve structural and physicochemical properties. In this work, N,S-GQDs were prepared through a high-temperature pyrolysis method using L-cysteine and citric acid as the precursors. The morphology and structure of nanocomposites were identified by TEM, X-ray diffractometer (XRD), X-ray photoelectron spectrometer (XPS), and FTIR. The as-prepared N,S-GQDs show bright blue fluorescence with satisfactory fluorescence quantum yield. N,S-GQDs display significant response to riboflavin, achieving a low detection limit of 27[Formula: see text]nM. The reaction of N,S-GQDs to riboflavin is mainly governed by static quenching. The detection of riboflavin in real samples has been performed to demonstrate its practical application. The obtained N,S-GQDs have low cytotoxicity and have been applied successfully in cell imaging.

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Using HPLC with mass-spectrometric detection for riboflavin determination in complex medicinal forms

Cited by 3 publications

References 2 publications

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

A Novel Direct Method for Determination of Riboflavin in Alcoholic Fermented Beverages

N,S Co-Doped Graphene Quantum Dots for Detection of Riboflavin and Cell Imaging

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