Using hydrogen isotope incorporation as a tool to unravel the surfaces of hydrogen-treated nanodiamonds

Nehlig, Émilie; Garcia‐Argote, Sébastien; Feuillastre, Sophie; Moskura, Mélanie; Charpentier, Thibault; Schleguel, Michel; Girard, Hugues A.; Arnault, Jean-Charles; Pieters, Grégory

doi:10.1039/c9nr01555h

Cited by 12 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We measured hydrodynamical diameters of DND clusters ranging from about 60 nm to about 90 nm for H-DND and T-DND colloidal suspensions, respectively ( Supplementary Table S2). These results are in agreement with previous colloidal studies on hydrogenated DND [21,24] and also with the transmission electron microscopy observations we reported in Ref. [16] (see Figure 4a in this reference), showing H-DND aggregates of ≈50 nm in size at the cell membrane.…”

Section: Characterization Of the Hydrogenated And Tritiated Nanodiamosupporting

confidence: 94%

“…As-received manually milled DND were first pre-oxidized to remove native C-H groups to achieve a more quantitative control of tritium added to the surface [21]. To this aim, 100 mg was placed in an alumina crucible under air for 1 h 30 min at 550 • C. Then, 30 mg of these pre-oxidized DND were annealed for 4 h at 550 • C, using the same process that for H-DND with 10% T 2 and 90% H 2 gas mixture (in order to obtain the desired activity).…”

Section: Mixture Of T-dnd and H-dnd (Later Denoted T-dnd To Simplify)mentioning

confidence: 99%

“…After hydrogenation or tritiation by annealing using molecular gases (see methods and [21]), we suspended the treated DND powder in distilled water by sonication. We measured hydrodynamical diameters of DND clusters ranging from about 60 nm to about 90 nm for H-DND and T-DND colloidal suspensions, respectively ( Supplementary Table S2).…”

Section: Characterization Of the Hydrogenated And Tritiated Nanodiamomentioning

confidence: 99%

“…Indeed, Riojas et al [20] showed that hydroxylated 7-nm sized DND further functionalized with radiolabeled amino groups are efficiently eliminated in urines if the solution is filtered before being intravenous injected.In this work, we describe an original method that we developed to treat ES in vivo by injecting DND:siRNA complexes, and to determine the organ distribution of these DND. To this aim, we used DND labeled with tritium by annealing method [21]. We show that siRNA (i) can be loaded onto hydrogenated or tritiated DND (H-DND and T-DND, respectively), (ii) can efficiently inhibit EWS-FLI1 in ES tumor model xenografted on mice and (iii) that the organ distribution and elimination of T-DND can be measured thanks to its radioactivity.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution

et al. 2020

Self Cite

View full text Add to dashboard Cite

Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. polymers or dendrimers [5]), inorganic (e.g., metallic nanoparticles such as gold, iron, titanium [4]) or mineral like clay [6], silica nanoparticles [7] and nanodiamonds [8,9]. Most inorganic nanocarriers present a good-to-high chemical stability; they have a low toxicity at therapeutic dose of the drug:carrier conjugate and are able to deliver their cargo compounds into cells. Nevertheless, the high stability of inorganic nanoparticles goes with the fact that they are not biodegradable, and for pharmacological applications, this can be a limiting factor. Therefore, for the safe development of these particles, it is crucial to determine their possible elimination pathways after administration and study how they are distributed in the body.Here, we report on the use of cationic detonation nanodiamonds (DND) for the delivery of siRNA directed against Ewing sarcoma (ES) junction oncogene EWS-FLI1 to ES tumor xenografted on mice. Ewing sarcoma is a rare bone and soft tissue cancer [10] which is caused in the vast majority of cases by the formation of EWS-FLI1 oncogene. To carry siRNA, DND surface needs to be modified to be able to bind negatively charged nucleic acids by electrostatic interactions. One strategy relies on cationic polymer coating of diamond surface, which is either done by electrostatic interaction [9,11] or by covalent grafting [12,13]. However, polymer coating may lead to the formation of large aggregates by crosslinking DND to other DND via polymer chains bridging. Another strategy, the one selected for this study, is based on the direct surface modification of DND using hydrogen gas combined with microwave plasma or with annealing method. Both hydrogenation methods were recently compared [14]. It was shown that after such reductive treatment, the hydrogenated DND (H-DND) can be dispersed in water, and acquire a positive surface charge characterized by a zeta potential of ...

show abstract

Section: Characterization Of the Hydrogenated And Tritiated Nanodiamosupporting

confidence: 94%

Section: Mixture Of T-dnd and H-dnd (Later Denoted T-dnd To Simplify)mentioning

confidence: 99%

Section: Characterization Of the Hydrogenated And Tritiated Nanodiamomentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The excellent nuclear properties of tritium for NMR detection were recognized early in the development of NMR spectroscopy, 7 but applications of modern experimental methods to tritiated materials have subsequently been limited to a small number of examples on organic solids. 8,9 In the case of irradiated ceramics, the radioactivity hazard is amplified by the continuous release of tritiated gases from the matrix during experiments. 4 Recent advances in NMR instrumentation adapted for volatile, highly radioactive materials open the way to the use of NMR spectroscopy as a nondestructive, isotope-selective approach for analyzing irradiated samples.…”

Section: Introductionmentioning

confidence: 99%

Isotope‐specific analysis of neutron‐irradiated lithium aluminate ceramics by nuclear magnetic resonance spectroscopy

et al. 2020

View full text Add to dashboard Cite

The effects of neutron irradiation on lithium aluminate ceramics and the transport and incorporation of tritium into surrounding components are primary concerns in the development of materials for use in tritium production. The high specific activity and volatility of the tritium in activated samples greatly increase the challenges of experimental analysis. This paper reports the determination of structural forms and concentrations of 3 H and 6 Li in irradiated and nonirradiated LiAlO 2 by nuclear magnetic resonance spectroscopy. The experiments find tritium uniformly distributed in hydroxyl groups bridging aluminum centers and lithium in four-and six-coordinated sites. These results demonstrate that measurements can be performed in a nondestructive, safe manner to obtain isotope-selective, quantitative information even on highly hazardous materials.

show abstract

Advanced Materials Designed with Nanodiamonds

Arnault

2020

Synthesis and Applications of Nanocarbons

View full text Add to dashboard Cite

Using hydrogen isotope incorporation as a tool to unravel the surfaces of hydrogen-treated nanodiamonds

Abstract: We report a robust method for the labelling of detonation nanodiamonds with hydrogen isotopes and demonstrate the usefulness of isotope incorporation in investigating the surface chemistry of such nanomaterials.

Cited by 12 publications

References 44 publications

Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution

Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution

Isotope‐specific analysis of neutron‐irradiated lithium aluminate ceramics by nuclear magnetic resonance spectroscopy

Advanced Materials Designed with Nanodiamonds

Contact Info

Product

Resources

About