Using in vivo intact structure for system-wide quantitative analysis of changes in proteins
Ahrum Son,
Hyunsoo Kim,
Jolene K. Diedrich
et al.
Abstract:Mass spectrometry-based methods can provide a global expression profile and structural readout of proteins in complex systems. Preserving the in vivo conformation of proteins in their innate state is challenging during proteomic experiments. Here, we introduce a whole animal in vivo protein footprinting method using perfusion of reagents to add dimethyl labels to exposed lysine residues on intact proteins which provides information about protein conformation. When this approach is used to measure dynamic struc… Show more
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