Using molecular detection for the diversity and occurrence of cyanobacteria and 2-methylisoborneol-producing cyanobacteria in an eutrophicated reservoir in northern China

Qiu, Pengfei; Chen, Youxing; Li, Chenjie; Huo, Da; Bi, Yonghong; Wang, Jianbo; Li, Yunchuang; Li, Renhui; Yu, Ge

doi:10.1016/j.envpol.2021.117772

Cited by 13 publications

(1 citation statement)

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“…High-throughput sequencing, including amplicon sequencing and metagenomic sequencing, could rapidly detect unculturable lowabundance microorganisms in the environment (Liu et al, 2021). Amplicon sequencing is widely used in the study of microbial diversity, especially for toxic or odor-producing cyanobacteria (Cristina Casero et al, 2019;Qiu et al, 2021). However, the identification of species by amplicon sequencing can only reach a genus-level resolution, and the selection of primers and the determination of the number of PCR cycles during sequencing have a large impact on the sequencing results.…”

Section: Introductionmentioning

confidence: 99%

Producers and drivers of odor compounds in a large drinking-water source

Qiu

Zhang

et al. 2023

Front. Ecol. Evol.

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IntroductionTaste and odor (T&O) problems have been affecting drinking water safety. As a eutrophicated drinking water reservoir in Tianjin city, the Yuqiao Reservoir was threatened by 2-MIB and geosmin in recent years.MethodsIn this study, quantile regression analysis and metagenome were used to quickly and accurately screen the producers and drivers of 2-MIB and geosmin in this reservoir.ResultsThe mean concentrations of 2-MIB and geosmin in the four-year were 103.58 ± 128.13 ng/L and 14.29 ± 27.95 ng/L, respectively. 2-MIB concentrations were higher in summer and autumn, with a bimodal variation throughout the year. Geosmin concentrations showed a decreasing trend from year to year from 2018 to 2021. Metagenome revealed that Pseudanabaena sp. dqh15, Microcoleus pseudautumnalis Ak1609, Pseudanabaena limnetica, and Planktothricoides raciborskii were the 2-MIB-producers, while Streptosporangium caverna and Dolichospermum circinale were the geosmin-producers. Multivariate quantile regression analysis indicated Pseudanabaena sp. and CODMn were the best predictors of 2-MIB concentrations, temperature and CODMn were the most useful parameters for describing geosmin concentration change. 2-MIB concentrations increased with the increase of Pseudanabaena sp. cell density and CODMn. Geosmin concentrations were higher at harsh temperatures and increased with higher CODMn. CODMn was significantly and positively correlated with the biosynthesis of secondary metabolites synthesis and terpenoid backone biosynthesis pathway. Both quantile regression and metagenome results showed that CODMn was an important driver of odor compounds.DiscussionMetagenome achieved higher resolution of taxonomic annotation than amplicons to identify odor-producers, which helps us to understand the main taxa of odor-producing microorganisms in Chinese water bodies and the genetic basis of odor compounds in microorganisms. Understanding the sources and drivers of odor compounds was useful for improving taste and odor problem management. This is the first time that the main odor-producing microorganisms in water bodies have been resolved by microbial metagenomic functional gene prediction.

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Section: Introductionmentioning

confidence: 99%