Using nematodes in soil ecotoxicology

Sochová, Ivana; Hofman, Jakub; Holoubek, Ivan

doi:10.1016/j.envint.2005.08.031

Cited by 107 publications

(54 citation statements)

References 87 publications

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“…6A). The correlations for toxicities between C. elegans and rodents make the case of inclusion of C. elegans for toxicity assessment (Dengg and van Meel, 2004; Sochova et al ., 2006). Our results suggest that tested compounds could use for hyphal inhibition in animals with low dosage.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Manoharan

Lee

2018

Microbial Biotechnology

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SummaryCertain pathogenic bacteria and yeast form biofilms on biotic and abiotic surfaces including medical devices and implants. Hence, the development of antibiofilm coating materials becomes relevant. The virulence of those colonizing pathogens can be reduced by inhibiting biofilm formation rather than killing pathogens using excessive amounts of antimicrobials, which is touted as one of the main reasons for the development of drug resistance. Candida albicans is an opportunistic fungal pathogen, and the transition of yeast cells to hyphal cells is believed to be a crucial virulence factor. Previous studies have shown that indole and its derivatives possess antivirulence properties against various bacterial pathogens. In this study, we used various indole derivatives to investigate biofilm‐inhibiting activity against C. albicans. Our study revealed that 7‐benzyloxyindole, 4‐fluoroindole and 5‐iodoindole effectively inhibited biofilm formation compared to the antifungal agent fluconazole. Particularly, 7‐benzyloxyindole at 0.02 mM (4.5 μg ml−1) significantly reduced C. albicans biofilm formation, but had no effect on planktonic cells, and this finding was confirmed by a 2,3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide (XTT) assay and three‐dimensional confocal laser scanning microscopy. Scanning electron microscopy analyses revealed that 7‐benzyloxyindole effectively inhibited hyphal formation, which explains biofilm inhibition. Transcriptomic analysis showed that 7‐benzyloxyindole downregulated the expressions of several hypha/biofilm‐related genes (ALS3,ECE1,HWP1 and RBT1). A C. albicans‐infected Caenorhabditis elegans model system was used to confirm the antivirulence efficacy of 7‐benzyloxyindole.

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Section: Discussionmentioning

confidence: 99%

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Manoharan

Lee

2018

Microbial Biotechnology

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“…Nematodes are the most abundant metazoan organisms in the soil; approximately 7.6 × 10 5 and 2.9 × 10 7 individuals per square meters inhabit desert and deciduous forest ecosystems, respectively (Volz 1951;Sohlenius 1980). Due to their prominent role in the soil processes, as a decomposition of organic matter and energy flows (Sohlenius 1980;Yeates et al 2003), they represent irreplaceable components of the soil food web (Sochová et al 2006). It has been estimated that approximately 40% of nutrient mineralisation in ecosystem is currently coming from nematodes and other soil microbe-feeding fauna (De Ruiter et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Nematode community structure in the vicinity of a metallurgical factory

Šalamún

Renčo

Miklisová

et al. 2011

Environ Monit Assess

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Soil nematode communities (taxa composition, trophic structure, ecological indices) in the area of metallurgical factory (Oravské ferozliatinárske závody) in Široká, Northern Slovakia were investigated in 2009. The factory belongs to main sources of emissions originated by ferroalloy production in this region. Four sites (meadows) were selected in a downwind direction from the factory: site A was located 0.85 km far from the factory, and the other sites were maintained in approximately 2-km intervals from each other. Chemical analysis of soil samples showed low concentrations of heavy metals (As, Cd, Cr, Cu, Ni, Pb and Zn), with all values being under Slovak limit concentrations of heavy metals in soils. Only the Cd content in the soil sample from site A slightly exceeded the allowable threshold, but it was decreasing with the distance from the factory, similarly as remaining metals except Cr, with slightly increasing trend of concentration. Within 64 identified nematode genera, the Helicotylenchus, Paratylenchus, Pratylenchus, Acrobeloides, Cephalobus and Rhabditis were most common and eudominant. This was clearly reflected on the

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“…Because organic matter (OM) content and C:N ratio can be measured quickly and easily, soil ecosystem evaluation is often confined to these parameters. Nematode community indices are especially useful in detecting impacts of various soil treatments on nematode communities and soil quality (Bongers, 1999;Sochová et al, 2005;McSorley, 2011;Bastow, 2012). However, calculating these indices requires specialist taxonomical knowledge and is labor intensive, which makes it impracticable for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Impact of castor meal on root-knot and free-living nematodes

Ritzinger

Ley

Ploeg

et al. 2014

Sci. agric. (Piracicaba, Braz.)

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Soil amendment may enhance soil quality as well as reduce plant-parasitic nematode. Despite the many applications already undertaken using castor meal, its efficiency in controlling root-knot nematodes (RKN, Meloidogyne incognita) when applied to melon (Cucumis melo) is still not clear. Three different amounts of castor meal (Ricinus communis) applied were evaluated in microplots planted with melon either with or without RKN. The impact of castor meal on soil free-living nematode communities was also determined. Total nematode genera richness was estimated as 37 for the entire set of microplots sampled across both sampling dates. Rarefaction analysis resulted in 12 collector's curves out of the total of 30 that reached the horizontal asymptote. Univariate ANOVA with two factors yielded differences (p ≤ 0.05) only with regard to the time factor. Simpson, Shannon, Evenness and Equitability indices showed a trend toward moderate increases by the end of the experiment, while the other indices were higher for tomato in pre-transplant sampling compared to harvest. Nematode community and diversity changed during the course of the experiment, although there was substantial confounding heterogeneity within and between the factorial combinations from the beginning. Root knot population was not reduced by the castor meal but increased throughout the period, regardless of treatment. RKN reduced melon yield, number and weight of melon.

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Using nematodes in soil ecotoxicology

Cited by 107 publications

References 87 publications

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Nematode community structure in the vicinity of a metallurgical factory

Impact of castor meal on root-knot and free-living nematodes

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