Using of bioinformatics and computer morphometry in study of Fusarium spp. causing potato dry rot

Abstract

Nadezhda Zhilinskaia E-mail: jilinskie@mail.ru Introduction. The purpose of research -to evaluate the possibility of using the methods of computer morphometry and bioinformatic analysis in studying of reactive changes in Fusarium spp. causing potato dry rot.Materials and methods. Tubers of "Nevski" potato with Fusarium contamination signs were selected. Three areas of the damaged tuber potatoes were studied: the epicenter of contaminated plant tissue; peripheral area of contamination; plant tissue outside of contamination epicenter. Morphological research and computer morphometry of fixed preparations of microorganisms stained by 1,0% methylene blue solution were made under magnification x 400, x 1000.Results and discussion. Macroconidium morphometric characteristics of Fusarium spp. (square, perimeter, length, number) were obtained by automatic image analyzer "Micros". The algorithm and the computer program based on morphometric analysis of Fusarium spp. macroconidium cells causing potato dry rot was created to study bioinformatic cell characteristics.The following information characteristics of fungal cells have been calculated: H -information entropy characterizing the real structural diversity; H max -the storage capacity characterizing the maximum structural time-diversity of biological system; R (%) -information redundancy coefficient characterizing the relative organization of biosystem.The Fusarium macroconidium cells have demonstrated low values of H (0,002) compared to H max (0,5). The values of R in Fusarium cells were 99,40% -99,69% thus demonstrated a high degree of orderliness and relative organization in microbial cellss. Our research proves the maximum adaptation level of Fusarium cells as parasites in biocenosis with plant cells. It should be expected that in the case of the reaction of Fusarium cells on chemical or biological preparations the value of H should increase up to values of H max , and the value of R will decrease. It's may be occured as a result of disruption structural and functional integrity of Fusarium cell system.Conclusions. The method of bioinformatic analysis of Fusarium spp. cells causing potato dry rot can be recommended as a testing system in development of new plant protecting preparations.

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