Using precursor ion scan of 184 with liquid chromatography-electrospray ionization-tandem mass spectrometry for concentration normalization in cellular lipidomic studies

Differences in lipidomics may be potential biomarkers for early diagnosis of pancreatic cancer

“…Lipidomics was one of the metabolomics approach that focuses on lipids and was a promising technique for overviewing lipid profiles in blood and tissues 5 - 7 . It has been reported that the denovo synthesis of fatty acids was significantly higher in tumor cells and tumor tissues than that of normal cells to meet the needs of rapid growth and expansion of cells.…”

Cell metabolomics analyses revealed a role of altered fatty acid oxidation in neurotoxicity pattern difference between nab-paclitaxel and solvent-based paclitaxel

“…Procedures for the collection of cell samples were similar to that mentioned before except that SH-SY5Y cells were seeded in 60-mm dishes at a seeding density of 5 × 10 5 cells/dish. The extraction procedure and the precursor ion scan of 184 method described by Chao et al [23] for concentration normalization were applied with necessary adjustments. Briefly, a volume of 500 μL of premixed ice-cold mass-grade methanol and mass-grade water (4˚C, 4:1) was added to cell pellets, mixed at 1000 rpm for 2 minutes by using 2010 Geno/Grinder (SPEX, Metuchen, NJ, USA).…”

Advances in analytical tools and current statistical methods used in ultra-high-performance liquid chromatography-mass spectrometry of glycero-, glycerophospho- and sphingolipids

“…AA076, [78] and QOrbitrap [3], [5], [9], [17][18][19][20], [22], [26], [27], [31], [34], [35], [46], [47], [49][50][51], [55][56][57][58], [60], [62][63][64][65], [72], [77], [79] were used. However, some studies settled for low-resolution MS, mostly with a triple quadrupole instrument [7], [8], [23], [24], [30], [41][42][43]. Most rarely, pseudotargeted methods [21], [30], [54] (i.e.…”

“…In lipidomics, a wide range of articles introduce new or improved methods which are validated with UHPLC-MS, capillary-UHPLC-MS (nUHPLC-MS or nano-UHPLC-MS) instruments (Table 2). Most of these studies concentrate on sample preparation [14], [20], [46], [66], development of chromatographic methods [9], [22], [27], [45], MS/MS identification [7], [16], [23], [27], computational methods for improvement of measurement performance [17], and data analysis [21], [25], [96]. Recent challenges in HPLC-MS seem to be fluctuation (repeatability) of analysis [97], [98] results, which hinder reliable identification and interlaboratory tests of lipids.…”