Using Proteomic Analysis of the Human Amniotic Fluid to Identify Histologic Chorioamnionitis

Buhimschi, Irina A.; Zambrano, Eduardo; Pettker, Christian M.; Bahtiyar, Mert O.; Paidas, Michael J.; Rosenberg, Victor; Thung, Stephen; Salafia, Carolyn M.; Buhimschi, Catalin S.

doi:10.1097/aog.0b013e31816102aa

Cited by 54 publications

(60 citation statements)

References 45 publications

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“…However, a limitation of this study is that we are unable to attribute fertilization to oocyte quality only, although we Fig. 2 Spectral counts of heparan sulfate proteoglycan perlecan in the follicular fluids containing fertilized oocytes (fFF) and non-fertilized oocytes (nfFF) in each patient (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) tried to minimize the influence of sperm quality using ICSI. An increased number of samples would enable the use of a proteomics analysis to identify distinctive biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…Proteomics is helpful not only for identifying new disease-related biomarkers, but also for improving understanding of the pathophysiology of diseases. The application of proteomic analyses in medical research is intended to enrich a targetsetofdisease-relatedproteins.Inthegynecologicfield,this technique has been applied in the analysis of amniotic [5,10] and follicular fluid [3,11,18].…”

Section: Introductionmentioning

confidence: 99%

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A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Bayasula

Iwase

Kobayashi

et al. 2013

J Assist Reprod Genet

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Purpose Human follicular fluid constitutes the microenvironment of follicles and includes various biological active proteins that can affect follicle growth and oocyte fertilization. Conducting proteomic evaluations of human follicular fluid may be helpful for identifying potential biomarkers possibly possessing a predictive value for oocyte quality and the success of in vitro fertilization. Method We performed proteomic profiling of human follicular fluids containing oocytes that were fertilized and resulted in pregnancy and follicular fluids containing oocytes that were not fertilized in the same patients undergoing intracytoplasmic sperm injection using the LTQ Orbitrap coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses. ResultsWe identified a total of 503 proteins in human follicular fluids containing fertilized and non-fertilized oocytes obtained from 12 patients. We also found that 53 proteins exhibited significantly different spectral counts between the two groups, including heparan sulfate proteoglycan perlecan, which showed significant upregulation in the follicular fluids containing fertilized oocytes in comparison with that observed in the follicular fluids containing non-fertilized oocytes. Conclusion Our results suggest a possibility that proteins identified by LC/MS/MS in follicular fluid might not only be involved in folliculogenesis, but also function as biomarkers possessing predictive potential for oocyte maturation and the success of IVF when their expression levels are significantly different between fertilized and non-fertilized oocytes, although no distinctive biomarkers were identified in the current study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Bayasula

Iwase

Kobayashi

et al. 2013

J Assist Reprod Genet

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“…Therefore, the presence of Candida Mn antigen was observed very early in the Mn-positive group and bound with higher levels of some of the inflammatory markers that have been demonstrated to be associated with histological chorioamnionitis, funisitis, and early-onset neonatal sepsis (25,33). It is possible to speculate a vertical transmission of Candida spp.…”

Section: Discussionmentioning

confidence: 99%

Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates

et al. 2013

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Background: Candida mannan (Mn) detection in bronchoalveolar lavage fluid (BALF) was shown to be useful for earlier identification and preemptive therapy targeting in preterm infants at high risk of invasive Candida infection. We investigated whether early detection of Candida Mn in BALF is associated with the presence of some neutrophilic products, as markers of prenatal infection/inflammation. Methods: BALF specimens were collected during the first 48 h of life from mechanically ventilated preterm newborns. Samples were analyzed by high-performance liquid chromatography-electrospray ionization-mass spectrometry. The relative amounts of α-defensins 1-4 and S100A proteins were measured by extracted ion current peak area. Absolute and differential white cell counts in BALF were obtained. Mn antigen concentrations were determined by the Platelia Candida antigen kit. results: Twenty-five studied neonates were divided into two groups: Mn-positive group and Mn-negative group. Levels of α-defensins 1-4 and S100A12 were significantly higher in the Mn-positive group than in the Mn-negative group. Moreover, positive significant correlations between the absolute number of neutrophils and the levels of α-defensins 1-4 and S100A8 were observed. conclusion: The detection of Mn antigen in BALF of preterm infants is consistent with evidence of an innate immune response in their lungs as demonstrated by higher levels of α-defensins and S100A proteins.

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“…Because bacterial cultures and Gram stain have known limitations in the diagnosis of chorioamnionitis, we confirmed the presence of intra-amniotic inflammation secondary to IAI by using mass spectrometry [4, 15 ]. The methodology for generation of the proteomic Mass Restricted (MR) score has been previously described.…”

Section: Methodsmentioning

confidence: 99%

Compartmentalization of acute phase reactants Interleukin-6, C-Reactive Protein and Procalcitonin as biomarkers of intra-amniotic infection and chorioamnionitis

et al. 2015

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Background The arsenal of maternal and amniotic fluid (AF) immune response to local or systemic infection includes among others the acute-phase reactants IL-6, C-reactive protein (CRP) and procalcitonin (PCT). If these molecules can be used as non-invasive biomarkers of intra-amniotic infection (IAI) in the subclinical phase of the disease remains incompletely known. Methods We used time-matched maternal serum, urine and AF from 100 pregnant women who had an amniocentesis to rule out IAI in the setting of preterm labor, PPROM or systemic inflammatory response (SIR: pyelonephritis, appendicitis, pneumonia) to infection. Cord blood was analyzed in a subgroup of cases. We used sensitive immunoassays to quantify the levels of inflammatory markers in the maternal blood, urine and AF compartment. Microbiological testing and placental pathology was used to establish infection and histological chorioamnionits. Results PCT was not a useful biomarker of IAI in any of the studied compartments. Maternal blood IL-6 and CRP levels were elevated in women with subclinical IAI. Compared to clinically manifest chorioamnionitis group, women with SIR have higher maternal blood IL-6 levels rendering some marginal diagnostic benefit for this condition. Urine was not a useful biological sample for assessment of IAI using either of these three inflammatory biomarkers. Conclusions In women with subclincal IAI, the large overlapping confidence intervals and different cut-offs for the maternal blood levels of IL-6, CRP and PCT likely make interpretation of their absolute values difficult for clinical decision-making.

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Using Proteomic Analysis of the Human Amniotic Fluid to Identify Histologic Chorioamnionitis

Abstract: II.

Cited by 54 publications

References 45 publications

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates

Compartmentalization of acute phase reactants Interleukin-6, C-Reactive Protein and Procalcitonin as biomarkers of intra-amniotic infection and chorioamnionitis

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