Using synergistic exogenous phytohormones to enhance somatic embryogenesis from leaf explants of a<i>Eucalyptus grandis</i>clone

Nakhooda, Muhammad; Mandiri, Eshani

doi:10.2989/20702620.2015.1108620

Cited by 8 publications

(2 citation statements)

References 27 publications

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“…Because of these similarities, SE is not only the ultimate reflection of cell totipotency but also an excellent model system for studying the early stages of embryogenesis (Zimmerman, 1993 ; Quiroz-Figueroa et al, 2006 ; Nowak et al, 2015 ). Currently, SE is used for seedling propagation (Yang et al, 2013 ; Nakhooda and Mandiri, 2016 ), germplasm conservation (Bakhshaie et al, 2010 ; Pullman et al, 2016 ), artificial seed production (Micheli and Standard, 2016 ) and breeding for genetic engineering (Corredoira et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Small RNA and Transcriptome Sequencing Reveal a Potential miRNA-Mediated Interaction Network That Functions during Somatic Embryogenesis in Lilium pumilum DC. Fisch.

et al. 2017

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Plant somatic embryos are widely used in the fields of germplasm conservation, breeding for genetic engineering and artificial seed production. MicroRNAs (miRNAs) play pivotal roles in somatic embryogenesis (SE) regulation. However, their regulatory roles during various stages of SE remain unclear. In this study, six types of embryogenic samples of Lilium pumilum DC. Fisch., including organogenic callus, embryogenic callus induced for 4 weeks, embryogenic callus induced for 6 weeks, globular embryos, torpedo embryos and cotyledon embryos, were prepared for small RNA sequencing. The results revealed a total of 2,378,760 small RNA reads, among which the most common size was 24 nt. Four hundred and fifty-two known miRNAs, belonging to more than 86 families, 57 novel miRNAs and 40 miRNA*s were identified. The 86 known miRNA families were sorted according to an alignment with their homologs across 24 land plants into the following four categories: 23 highly conserved, 4 moderately conserved, 15 less conserved and 44 species-specific miRNAs. Differentially expressed known miRNAs were identified during various stages of SE. Subsequently, the expression levels of 12 differentially expressed miRNAs and 4 targets were validated using qRT-PCR. In addition, six samples were mixed in equal amounts for transcript sequencing, and the sequencing data were used as transcripts for miRNA target prediction. A total of 66,422 unigenes with an average length of 800 bp were assembled from 56,258,974 raw reads. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment indicated that 38,004 and 15,497 unigenes were successfully assigned to GO terms and KEGG pathways, respectively. Among the unigenes, 2,182 transcripts were predicted to be targets for 396 known miRNAs. The potential targets of the identified miRNAs were mostly classified into the following GO terms: cell, binding and metabolic process. Enriched KEGG analysis demonstrated that carbohydrate metabolism was the predominant pathway in Lilium SE. Thus, we performed systemic characterization, homology comparisons and profiling of miRNA expression, and we constructed an miRNA-target network during Lilium SE for the first time. Our findings establish a foundation for the further exploration of critical genes and elucidation of SE in Lilium.

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Section: Introductionmentioning

confidence: 99%

Small RNA and Transcriptome Sequencing Reveal a Potential miRNA-Mediated Interaction Network That Functions during Somatic Embryogenesis in Lilium pumilum DC. Fisch.

et al. 2017

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“…A área de cultura de tecidos vegetais para a indução in vitro de calos em Eucalyptus grandis vem sendo estudada visando principalmente à otimização de protocolos para a regeneração e propagação de plantas e como ferramenta na área de transformação genética (Fernando et al, 2016;Hajari et al, 2006;Li et al, 2015a;Nakhooda and Mandiri, 2016;Oberschelp et al, 2015 (Fernando et al, 2016;Hajari et al, 2006;Nakhooda and Mandiri, 2016;Oberschelp et al, 2015 grandidentata) e soja (Möller et al, 2006a;Yamagishi et al, 2012Yamagishi et al, , 2013Yamagishi et al, , 2015 em calos com a coloração esbranquiçada e recém-formados. Nesses casos, foi possível observar a presença dos elementos traqueais pela birrefringência exibida por essas células.…”

Section: Obtenção In Vitro E Caracterização Morfológica Dos Elementosunclassified

Perfil transcricional da xilogênese em <i>Eucalyptus grandis</i>

Pinto¹

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Transcriptional profile of xylogenesis in Eucalyptus grandis Eucalyptus (Eucalyptus spp.) is the most important commercial tree in Brazil, playing a significant role in the pulp and paper industry. An increase in the yield of cellulosic pulp extraction and a reduction of production cost are the main objectives of the eucalyptus breeding programs. As the delignification process is fundamental for the extraction of cellulose, it is highly important to understand the biosynthesis and deposition of the secondary cell wall during xylogenesis. The differentiation of the tracheary elements is characterized by the deposition of the secondary cell wall, so studies of gene expression involving these xylem cells is an interesting strategy. Sequencing of the transcriptome of cells in suspension at different stages of development (meristematic cells, elongated cells and tracheal elements) was performed generating 348 million paired-end reads with 100 bp each, with most readings (80%) aligned and mapped against reference genome. The analysis of differentially expressed genes (DEG) identified a total of 3426, 1044 and 4665 DEGs in the comparisons M vs. A, A vs. T and M vs. T, respectively. Being 1316, 567, 2637 up-regulated genes and 2110, 476, 2028 down-regulated genes in the M/A, T/A, T/M, respectively. A total of 4229 genes were functionally annotated and mapped in 310 routes identified by the KEGG, yielding 40 sequences related to the phenylpropanoid route, the fifth most representative route. When DEGs with log2 fold change ≤-1.5 e log2 fold change ≥ 1.5 were considered, 34 metabolic routes were identified. From these, the main route was the phenylpropanoids pathway, with 14 sequences correlated to the enzyme lactoperoxidase. The GO terms enrichment was carried out and the networks of cellulose and xyloglucans biosynthesis, ethylene signaling routes, biogenesis routes and the plant cell wall organization and cell death, among others, were highlighted. Thirty-one transcription factors were identified, being 3 MYB transcription factors, 2 NAC transcription factors and 10 WRKY transcription factors. In addition, 21, 7 and 23 genes of the cellulose and hemicelluloses pathway and 24, 8 and 21 genes of lignin monomer synthesis route were found in the M vs. A, A vs. T and M vs. T comparisons, respectively. Besides this, encoding genes laccases and peroxidases were found, participating in the polymerization of lignin monomers. The results allowed a broader understanding of the transcriptional regulation of secondary cell wall biosynthesis in eucalyptus xylem cells and revealed new genes participating in xylogenesis. This set of transcriptional data will help in the accomplishment of genetic manipulations in order to obtain eucalyptus plants with extractability characteristics aspired by the industry.

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In Vitro Approaches for the Improvement of Eucalyptus

Kendurkar

Rangaswamy

2018

Biotechnologies of Crop Improvement, Volume 1

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Using synergistic exogenous phytohormones to enhance somatic embryogenesis from leaf explants of aEucalyptus grandisclone

Cited by 8 publications

References 27 publications

Small RNA and Transcriptome Sequencing Reveal a Potential miRNA-Mediated Interaction Network That Functions during Somatic Embryogenesis in Lilium pumilum DC. Fisch.

Small RNA and Transcriptome Sequencing Reveal a Potential miRNA-Mediated Interaction Network That Functions during Somatic Embryogenesis in Lilium pumilum DC. Fisch.

Perfil transcricional da xilogênese em <i>Eucalyptus grandis</i>

In Vitro Approaches for the Improvement of Eucalyptus

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