Using TRIS-Buffered Plasma-Activated Water to Reduce Pathogenic Microorganisms on Poultry Carcasses with Evaluation of Physicochemical and Sensory Parameters

Abstract

Foodborne diseases are mainly caused by the contamination of meat or meat products with pathogenic microorganisms. In this study, we first investigated the in vitro application of TRIS-buffered plasma-activated water (Tb-PAW) on Campylobacter (C.) jejuni and Escherichia (E.) coli, with a reduction of approx. 4.20 ± 0.68 and 5.12 ± 0.46 log10 CFU/mL. Furthermore, chicken and duck thighs (inoculated with C. jejuni or E. coli) and breasts (with natural microflora) with skin were sprayed with Tb-PAW. Samples were packed under a modified atmosphere and stored at 4 °C for 0, 7, and 14 days. The Tb-PAW could reduce C. jejuni on days 7 and 14 (chicken) and E. coli on day 14 (duck) significantly. In chicken, there were no significant differences in sensory, pH-value, color, and antioxidant activity, but %OxyMb levels decreased, whereas %MetMb and %DeoMb increased. In duck, we observed slight differences in pH-value, color, and myoglobin redox forms for the Tb-PAW, which were not perceived by the sensory test persons. With only slight differences in product quality, its application as a spray treatment may be a useful method to reduce C. jejuni and E. coli on chicken and duck carcasses.

Identification and classification of the genomes of novel Microviruses in poultry slaughterhouse

“…Written informed consent was obtained from all participants. (3) Air Samples: BioSamplers KIT (225-9595, SKC, Eighty Four, PA) were installed at approximately 1.5 meters above floor at the ventilation points in the slaughter area, pre-slaughter area, and caged area (3 sampling points in total). Using 0.5% BSA-PBS buffer at a flow rate of 8 mL/h, sampling was conducted for 12 hours per day at 110V.…”

“…(2) Combined nasal swab samples from 20 frontline slaughterhouse workers into one pool. (3) Combined air samples collected continuously for 3 days from each sampling point, creating one pool per sampling point. (4) Combined soil samples collected from each space (mixed samples with four or more points) into one pool.…”

“…They are also the key points for the gathering and transmission of pathogenic microorganisms (2). Due to the high density and mobility of poultry when entering the market or slaughterhouses, poultry comes from diverse sources and has varying hygienic conditions, and may carry multiple pathogenic microorganisms (3). The slaughter process is prone to contaminating the environment and the personnel involved.…”

A review on plasma‐activated water and its application in the meat industry

“…Grosse‐Peclum et al. (2023) reported that PAW caused slight differences in pH, color, and myoglobin redox forms when it interacted with chicken carcass, but these differences were not perceived during the sensory analysis.…”

Identification and classification of the genomes of novel microviruses in poultry slaughterhouse

“…They are also key points for the transmission of pathogenic microorganisms ( Benfu et al, 2021 ). Due to the high density and mobility of poultry when entering the market or slaughterhouses, poultry comes from diverse sources and has varying hygienic conditions, and may carry multiple pathogenic microorganisms ( Große-Peclum et al, 2023 ). The slaughter process is prone to contaminating the environment and the personnel involved.…”