Using UV spectrophotometry to analyze peritoneal dialyzate

Vasilevskii, A. M.; Коноплев, Г. А.

doi:10.1364/jot.71.000180

Cited by 4 publications

(3 citation statements)

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“…[12][13][14][15] Realizing such monitoring was proved in papers. 12,15,16 The study of many hemodialysis and peritoneal dialysis sessions has shown that there is a certain correlation between low molecular weight components, including creatinine, uric acid and urea, and concentration changes in effluent dialyzate, and corresponding UV extinction spectra changes in the 200 to 400-nm spectral region.…”

Section: Introductionmentioning

confidence: 99%

Peculiar character of dialyzate ultraviolet extinction spectra as an indicator of nucleic acid metabolism in humans

Vasilevsky

Konoplyov

2005

J. Biomed. Opt.

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UV extinction spectra of effluent dialyzate in the process of hemodialysis and peritoneal dialysis are studied. It is found that they have distinctive individual features unique to each patient. The shape of the spectrum remains virtually invariable for a given subject during the whole period of the study (more than a year) and does not depend on the treatment modality or other factors like sex, age, the stage of chronic renal failure, and concomitant illnesses. Absorption bands in two spectral regions--255 to 265 nm and 288 to 298 nm--account for the unique character of dialyzate spectra. Spectra classification based on the ratio of integral extinctions in these regions are introduced. It is assumed that the shape of the spectrum depends on the relative contents of plasma components. Statistical analysis of individual features of spectra for the groups of patients undergoing hemodialysis (78 people) and peritoneal dialysis (42 people) is carried out. It is revealed that the distribution of patients by the ratio of integral extinctions in the specified spectral regions adheres to the normal law. Presumably individual features of spectra could be related to uric acid and a biochemically unidentified substance with an absorption peak near 260 nm.

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Section: Introductionmentioning

confidence: 99%

Peculiar character of dialyzate ultraviolet extinction spectra as an indicator of nucleic acid metabolism in humans

Vasilevsky

Konoplyov

2005

J. Biomed. Opt.

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“…The interpretation of the second and the third peaks in the chromatograms was based on the authors' earlier research in the field of HD and PD effluent dialysate UVabsorption spectroscopy and optical dialysis monitoring [62][63][64]. According to the extensive experimental data, the most significant contribution in the UV absorption at 280 nm is made by uric acid, which is relatively abundant in blood plasma (and as a result in effluent dialysate) and is extremely optically active in the UV region with an absorption maximum of around 290 nm [63].…”

Section: Discussionmentioning

confidence: 99%

Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis

Kuznetsov¹,

Frorip²,

Sünter³

et al. 2022

Chemosensors

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Point-of-care testing (POCT) devices are becoming increasingly popular in the medical community as an alternative to conventional laboratory testing, especially for home treatments or other forms of outpatient care. Multiple-use chemical sensors with minimal requirements for disposables are among the most practical and cost-effective POC diagnostic instruments, especially in managing chronic conditions. An affordable, simple, and easy-to-use optical sensor based on fast protein liquid chromatography with direct UV absorption detection was developed for the rapid determination of the total protein concentration in effluent peritoneal dialysate and for the assessment of protein losses in end-stage renal disease (ESRD) patients on constant ambulatory peritoneal dialysis (CAPD). The sensor employs non-disposable PD-10 desalting columns for the separation of molecules with different molecular weights and a deep UV LED (maximum at 285 nm) as a light source for optical detection. The analytic procedure is relatively simple, takes 10–15 min, and potentially can be performed by patients themselves or nursing staff without laboratory training. Preliminary clinical trials on a group of 23 patients on CAPD revealed a good concordance between the protein concentrations in dialysate samples measured with the sensor and an automated biochemical analyzer; the mean relative error was about 10%, which is comparable with routine clinical laboratory methods.

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“…During the last decade, in connection with commercialization of the UV-technology for dialysis dose monitoring, new interest has appeared in the optical field. Some works, by the groups from Japan, related to spectroscopic analysis of uremic substances in dialysate have been presented [26,27] and additionally some papers from St. Petersburg [28]. Validation of a clinical prototype device [29] and the commercially available UV absorbance dialysis dose monitor have been also presented [30].…”

Section: Optical Online Monitoring Of Dialysis Dose 21 Background Of ...mentioning

confidence: 99%

Optical Online Monitoring of Uremic Toxins beyond Urea

Uhlin,

Fridolin

2023

Updates on Hemodialysis

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This chapter presents origin and physical basis of the optical method for traditional haemodialysis (HD) dose assessment, accepted as a valid bloodless, robust, automatic, in situ and online monitoring technology in clinical praxis. Dialysis dose Kt/V, total removed urea (TRU) and the nutrition parameters PCR, nPCR estimation from ultraviolet (UV) absorbance in the spent dialysate is explained. Since urea, a small water-soluble uremic solute and a surrogate marker for the efficiency of dialysis treatment to clear the blood of toxins and metabolic end products, is not representative for all retained uremic toxins removed with the modern dialysis care, new developments of optical online monitoring of uremic toxins, beyond urea, are discussed. Optical intradialytic monitoring of small-, middle- and protein-bound molecules’ removal, exemplified by marker molecules uric acid, beta-2 microglobulin and indoxyl sulphate, is described. A new concept and sensor technology for multi-component uremic toxins’ intradialytic optical monitoring of spent dialysate with some clinical examples are introduced. Drug interference studies during the optical dialysis monitoring and future directions in optical monitoring are included. Offered benefits will be more patient-centred, integrated and cost-efficient care, as feedback for clinicians helps to improve and personalize the treatment quality, minimizing costly adverse effects.

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Using UV spectrophotometry to analyze peritoneal dialyzate

Cited by 4 publications

References 0 publications

Peculiar character of dialyzate ultraviolet extinction spectra as an indicator of nucleic acid metabolism in humans

Peculiar character of dialyzate ultraviolet extinction spectra as an indicator of nucleic acid metabolism in humans

Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis

Optical Online Monitoring of Uremic Toxins beyond Urea

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