Using Water Chemistry, Isotopes and Microbiology to Evaluate Groundwater Sources, Flow Paths and Geochemical Reactions in the Death Valley Flow System, USA

Thomas, James M.; Moser, Duane P.; Fisher, James S.; Reihle, Jessica; Wheatley, A.; Hershey, Ronald L.; Baldino, Cristi; Weissenfluh, Darrick

doi:10.1016/j.proeps.2013.03.033

Cited by 15 publications

(10 citation statements)

References 15 publications

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“…Although these caveats in experimental design may amplify the differences between observed thermal spring and subsurface communities, it is important to note that in a study of Death Valley springs and their groundwater source, 21,794 partial 16S rRNA sequences were generated through pyrotagging and showed absolutely no overlap of the archaeal and bacterial communities in deep groundwater and surficial springs (Thomas et al, 2013 ). The lack of consensus between springs and the water that feeds them, as reported by Thomas et al ( 2013 ) and this study highlight the fact that we do not fully understand how microbial communities develop in the transition between surface spring and subsurface waters.…”

Section: Discussionmentioning

confidence: 99%

Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water

et al. 2014

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South Africa has numerous thermal springs that represent topographically driven meteoric water migrating along major fracture zones. The temperature (40–70°C) and pH (8–9) of the thermal springs in the Limpopo Province are very similar to those of the low salinity fracture water encountered in the South African mines at depths ranging from 1.0 to 3.1 km. The major cation and anion composition of these thermal springs are very similar to that of the deep fracture water with the exception of the dissolved inorganic carbon and dissolved O2, both of which are typically higher in the springs than in the deep fracture water. The in situ biological relatedness of such thermal springs and the subsurface fracture fluids that feed them has not previously been evaluated. In this study, we evaluated the microbial diversity of six thermal spring and six subsurface sites in South Africa using high-throughput sequencing of 16S rRNA gene hypervariable regions. Proteobacteria were identified as the dominant phylum within both subsurface and thermal spring environments, but only one genera, Rheinheimera, was identified among all samples. Using Morisita similarity indices as a metric for pairwise comparisons between sites, we found that the communities of thermal springs are highly distinct from subsurface datasets. Although the Limpopo thermal springs do not appear to provide a new window for viewing subsurface bacterial communities, we report that the taxonomic compositions of the subsurface sites studied are more similar than previous results would indicate and provide evidence that the microbial communities sampled at depth are more correlated to subsurface conditions than geographical distance.

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Section: Discussionmentioning

confidence: 99%

Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water

et al. 2014

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“…This enrichment was operated continuously for 6 months, with a media reservoir change 19 performed every 10-30 days. The dilution rate in the bioreactor amounted to one reactor volume 20 per day.…”

Section: Electrochemical Enrichment Bioreactor 31mentioning

confidence: 99%

“…interesting questions about the relevance of EET in energy-limited subsurface environments, 19 where the diverse host rocks may provide insoluble electron acceptors (or electron donors) that 20…”

Section: Implications 14mentioning

confidence: 99%

Isolation and characterization of electrochemically active subsurface Delftia and Azonexus species

Jangir

French

Momper

et al. 2016

Preprint

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1Continental subsurface environments can present significant energetic challenges to the resident 2 microorganisms. While these environments are geologically diverse, potentially allowing energy 3 harvesting by microorganisms that catalyze redox reactions, many of the abundant electron 4 donors and acceptors are insoluble and therefore not directly bioavailable. Extracellular electron 5 transfer (EET) is a metabolic strategy that microorganisms can deploy to meet the challenges of 6 interacting with redox-active surfaces. Though mechanistically characterized in a few metal-7 reducing bacteria, the role, extent, and diversity of EET in subsurface ecosystems remains 8 unclear. Since this process can be mimicked on electrode surfaces, it opens the door to 9 electrochemical techniques to enrich for and quantify the activities of environmental 10 microorganisms in situ.

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“…The regional hydrology of this system is well characterized due to U.S. Government studies of groundwater resources and potential contaminant transport from nearby underground nuclear tests. Specific sites for deep-life study in and around Death Valley were proposed based on prior microbiological observations of groundwater transmitted along fault zones with known seismic activity (Thomas et al, 2013;Fig. 5).…”

Section: Active Extensional Crustal Environmentsmentioning

confidence: 99%

Workshop to develop deep-life continental scientific drilling projects

et al. 2015

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Using Water Chemistry, Isotopes and Microbiology to Evaluate Groundwater Sources, Flow Paths and Geochemical Reactions in the Death Valley Flow System, USA

Cited by 15 publications

References 15 publications

Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water

Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water

Isolation and characterization of electrochemically active subsurface Delftia and Azonexus species

Workshop to develop deep-life continental scientific drilling projects

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