Using β-Lactamase to Trigger Supramolecular Hydrogelation

Yang, Zhimou; Ho, Pak‐Leung; Liang, Gaolin; Chow, Kin-Hung; Wang, Qigang; Cao, Yang; Guo, Zhihong; Xu, Bing

doi:10.1021/ja0675604

Cited by 205 publications

(150 citation statements)

References 22 publications

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“…[16,17] The b-lactamase enzymatic activity has recently also been applied to control formation of supramolecular hydrogels. [18] To extend the reporter utility of Bla to whole living animals is highly desirable but has been impeded by lack of a suitable substrate that can image the Bla activity in vivo. Herein, we report the first bioluminogenic substrate for Bla and demonstrate its application for in vivo imaging of the Bla activity.…”

mentioning

confidence: 99%

A Bioluminogenic Substrate for In Vivo Imaging of β‐Lactamase Activity

2007

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mentioning

confidence: 99%

A Bioluminogenic Substrate for In Vivo Imaging of β‐Lactamase Activity

2007

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“…[8,10,11] The released DTA dye fluoresces only weakly at 525 nm in the aqueous solution, presumably due to the fluorescence quenching effect of water (Figure 2 a). Upon addition of lysozyme fibrils, the DTA dye tends to bind to these highly ordered protein aggregates.…”

Section: Resultsmentioning

confidence: 99%

A Switch‐On Fluorescence Assay for Bacterial β‐Lactamases with Amyloid Fibrils as Fluorescence Enhancer and Visual Tool

Zou

Cheong

Chung

et al. 2010

Chemistry A European J

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Herein is described the development of a novel switch-on fluorescence assay for detecting β-lactamases. The fluorescence assay comprises two components: solid beads coated with a β-lactam antibiotic, which is linked to an environment-sensitive fluorophore (dansylaminothiophenol, DTA), and amyloid fibrils of hen lysozyme (acting as fluorescence enhancer and visual tool). In the presence of the clinically significant TEM-1 β-lactamase, the DTA-antibiotic complex on the solid beads is hydrolyzed, thus releasing the DTA dye into solution. The DTA dye is only weakly fluorescent in solution but gives strong green fluorescence upon binding to lysozyme fibrils. These strongly fluorescent DTA-bound fibrils can be easily visualized by the naked eye upon illumination of the sample with a simple UV lamp. The fluorescence assay can detect TEM-1 at low concentration (0.01 nM). In contrast, no observable fluorescence appears when the fluorescence assay is performed on samples without the TEM-1 β-lactamase.

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“…This has become a major goal in designing and engineering novel drug carrier nanofibers. [11][12][13] Polyoxalate (POX) is a biodegradable polymer that has been recommended for several medical devices such as absorbable sutures and controlled release vehicles. They are synthesized via a simple condensation reaction between diols and oxalyl chloride.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable poly(vinyl alcohol)/polyoxalate electrospun nanofibers for hydrogen peroxide-triggered drug release

Phromviyo

Lert-itthiporn

Swatsitang

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Release of drugs in a controlled and sustainable manner is of great interest for treating some inflammatory diseases, drug delivery, and cosmetics. In this work, we demonstrated the control release of a drug from composite nanofibers mediated by hydrogen peroxide. Composite nanofibers of polyvinyl alcohol (PVA)/polyoxalate (PVA/POX NFs) blended at various weight ratios were successfully prepared by electrospinning. Rhodamine B (RB) was used as a model of drug and was initially loaded into the POX portion. The morphology of NFs was characterized using scanning electron microscopy (SEM). The functional groups presented in the NFs were characterized using IR spectroscopy. In vitro release behavior and cell toxicity of nanofibers were also investigated using the MTT assay. The results indicated that POX content had a significant effect on the size and release profiles of nanofibers. Microstructure analysis revealed that sizes of PVA/POX NFs increased with increasing POX content, ranging from 214 to 422 nm. Release profiles of RB at 37 °C were non-linear and showed different release mechanisms. The mechanism of drug release depended on the chemical composition of the NFs. RB release from the NFs with highest POX content was caused by the degradation of the nanofiber matrix, whereas the RB release in lower POX content NFs was caused by diffusion. The NFs with POX showed a loss of structural integrity in the presence of hydrogen peroxide as seen using SEM. The MTT assay showed that composite nanofibers had minimal cytotoxicity. We anticipate that nanofibrous PVA/POX can potentially be used to target numerous inflammatory diseases that overproduce hydrogen peroxide and may become a potential candidate for use as a local drug delivery vehicle.

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Using β-Lactamase to Trigger Supramolecular Hydrogelation

Cited by 205 publications

References 22 publications

A Bioluminogenic Substrate for In Vivo Imaging of β‐Lactamase Activity

A Bioluminogenic Substrate for In Vivo Imaging of β‐Lactamase Activity

A Switch‐On Fluorescence Assay for Bacterial β‐Lactamases with Amyloid Fibrils as Fluorescence Enhancer and Visual Tool

Biodegradable poly(vinyl alcohol)/polyoxalate electrospun nanofibers for hydrogen peroxide-triggered drug release

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