Ustilago Species as a Cause of Central Line-Related Blood Stream Infection

McGhie, Travea; Huber, Thomas W.; Kassis, Christelle; Jinadatha, Chetan

doi:10.1097/maj.0b013e31826f56ed

Cited by 11 publications

(10 citation statements)

References 7 publications

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“…the gut mycobiome in human health. Some fungi coming from the diet or the environment are important to mediate interaction in the gut bacterial communities and regulate metabolic homeostasis [see [72][73]. This is the case for Eurotiales, Hypocreales, Saccharomycetales and Trichosporonales that are crucial parts of healthy mycobiome in control and lactobacillus-treated rats (see Figs.…”

Section: Discussionmentioning

confidence: 99%

“…As seen in rodents, a large variety of 'forgotten' odd fungi, including Ustilaginales and Ustilago sp. , are found in the human digestive tract [72][73][74][75]. So, it is not so doubtful, odd or surprising that fungal sequences such as Lecanicillium, Phallus and Ustilago are found in the fecal DNA from CTX-rat models.…”

Section: Profiling Microbiome In Five Different Groups Of Rats In Rel...mentioning

confidence: 99%

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<em>Lactobacillus</em> on Recovering of Immune-Damage by Chemotherapy (Cancer Treatment)

Yue¹,

Zhang²,

Bian³

et al. 2022

Preprint

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We demonstrated the role of natural probiotics 3L, 3 Lactobacilli, in the establishment of a strong and sustainable beneficial healthy gut flora, after chemotherapy through experimental results through in vivo model. Using rat CTX model (immunosuppression induced by cyclophosphamide), we suggested some new adjuvant to chemotherapy as drugs + lactobacillus treament. Further, we proposed a new probiotic formulation (L. acidophilus + L. casei + L. plantarum) to be explored in the prevention of health condition loss by alteration of the general immune system, in numerous studies that reported the use of probiotics involving Lactobacillus in the post-chemo or post-surgical procedures. Here, in our study, Illumina MiSeq sequencing was used to generate sequencing data from microbial genomic DNA libraries, which is appreciable to check for the effects of 3L on bacteria. Microbiome analysis, phylogenetic and classification reports, community data have supported the experiments and the results where 3L had strong beneficial effects on the microbiome. Further, the influence on specific metabolic pathways are assisted in deriving the conclusion of the study (use of 3L for cancer therapy) to the mode of action, mechanistically by correcting microbiota composition and enhancing specific gut metabolic functions.

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Section: Discussionmentioning

confidence: 99%

Section: Profiling Microbiome In Five Different Groups Of Rats In Rel...mentioning

confidence: 99%

<em>Lactobacillus</em> on Recovering of Immune-Damage by Chemotherapy (Cancer Treatment)

Yue¹,

Zhang²,

Bian³

et al. 2022

Preprint

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“…In past reports Pseudozyma spp was often initially misidentified as Cryptococcus laurentii by the VITEK 2 system (BioMérieuxInc, Durham, North Carolina, USA) 9. In most of the reported cases the organism was susceptible to amphotericin B, itraconzole and voriconazole but had variable susceptibility (sensitive, intermediate or resistant) to fluconazole.…”

Section: Discussionmentioning

confidence: 99%

Pseudozymaspp catheter-associated blood stream infection, an emerging pathogen and brief literature review

et al. 2014

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Pseudozyma spp are amorphic yeasts. They are commonly plant pathogens, but rarely cause invasive fungal disease in humans. Only three cases of central venous catheter (CVC)-associated blood stream infections due to this organism have been reported in the literature. Main underlying risk factors for Pseudozyma spp infection are bowel surgery, CVC and total parenteral nutrition. We present a rare case of Pseudozyma spp catheter-associated blood stream infection that was successfully treated with antifungal therapy and removal of CVC. It is important to recognise and differentiate this species from other yeasts as it may require the use of amphotericin B or voriconazole instead of fluconazole, to which the organism is variably resistant.

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“…The plant pathogen Ustilago (the agent of corn smut) and the closely related genus Pseudozyma are ubiquitous in the environment and are commonly regarded as laboratory contaminants. However, these organisms can be transmitted by the airborne route and have occasionally caused fungemia and central venous catheter infections in humans (5,7,(10)(11)(12)(13)(14)(15)(16).…”

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confidence: 99%

Cross-Reacting Ustilago maydis Causing False-Positive Cryptococcal Antigen Test Results

et al. 2017

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C ryptococcus neoformans is a yeast within the division Basidiomycota that may cause pulmonary and central nervous system (CNS) disease. Latex agglutination (LA) and lateral flow assay (LFA) for detection of cryptococcal polysaccharide antigen are sensitive and specific tests for diagnosis of invasive disease (1, 2); however, cross-reactivity has been described with Trichosporon asahii (3). Our objective was to determine if there is cross-reactivity with other Basidiomycete yeasts, including rare agents of human disease such as Rhodotorula, Sporobolomyces, and Ustilago spp. (4-8). Clinical isolates of Trichosporon, Rhodotorula, Sporobolomyces, and Ustilago spp. were retrieved from the Quebec provincial reference laboratory (LSPQ). Sporobolomyces, Trichosporon asahii, Rhodotorula glutinis, R. minuta, and R. mucilaginosa isolates were taxonomically confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) (Vitek MS; bioMérieux, Inc., Saint-Laurent, Quebec, Canada). Ustilago spp. and Rhodotorula slooffiae were confirmed by sequencing their ribosomal DNA D 1-D 2 and internal transcribed spacer (ITS) regions. Cryptococcus neoformans (LSPQ-16-A479650) and Candida albicans (ATCC 60433) strains were used as positive and negative controls, respectively. Growth in Sabouraud dextrose broth was confirmed by measuring the increase in optical density after 24 h (DensiCHEK Plus; bioMérieux, Inc.). Turbidity was adjusted to a 0.5 McFarland (MF) standard equivalent, and organism count/ml was determined by a hemocytometer. This turbidity corresponded to an average of 3.2 organisms/ml (standard deviation, 0.9 organisms/ ml), which is similar to the organism burden reported to occur in the cerebrospinal fluid of patients with cryptococcal meningitis (9). Serial 2-fold dilutions were performed to determine the cutoff for positivity. LA testing was performed using the CALAS cryptococcal antigen latex agglutination kit (Meridian Bioscience, Inc., Cincinnati, OH, USA). Flocculation was graded on a scale from 0 to 4ϩ, with 2 or higher representing a positive result. LFA was performed using the IMMY cryptococcal lateral flow assay (Immuno-Mycologics, Inc., Norman, OK, USA). Both assays were performed per the manufacturers' instructions, and assessment was made in a blind fashion. In total, 23 samples were tested, including 9 isolates of Rhodotorula spp., 5 isolates of Sporobolomyces salmonicolor, 3 isolates of Trichosporon asahii, and 3 isolates of Ustilago maydis (Table 1). Only C. neoformans, Trichosporon, and Ustilago isolates were unequivocally positive by both assays. One Rhodotorula mucilaginosa isolate and one Sporobolomyces salmonicolor isolate had discordant results; these isolates were weakly positive by the LA assay at titers of 1:1 and 1:4, respectively, but were negative by the LFA. All other isolates had concordant results between assays

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Ustilago Species as a Cause of Central Line-Related Blood Stream Infection

Cited by 11 publications

References 7 publications

<em>Lactobacillus</em> on Recovering of Immune-Damage by Chemotherapy (Cancer Treatment)

<em>Lactobacillus</em> on Recovering of Immune-Damage by Chemotherapy (Cancer Treatment)

Pseudozymaspp catheter-associated blood stream infection, an emerging pathogen and brief literature review

Cross-Reacting Ustilago maydis Causing False-Positive Cryptococcal Antigen Test Results

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