Uterine cervical punch biopsy specimens can be analyzed by ¹h mrs

Abstract

Biopsy specimens of the uterine cervix, including colposcopically directed punch biopsy specimens of females with atypical Papanicolaou smear tests, are suitable for analysis by magnetic resonance (MR) spectroscopy. A narrow lined lipid MR spectrum, characteristic of malignant tissue, is obtained from a 6-mm3 biopsy specimen of histologically confirmed squamous carcinoma of the cervix. In contrast, specimens containing inflammatory cells generate a broad component only centered at 1.3 ppm with a T2 relaxation value of less than 350 ms. Most biopsy specimens which contain dysplastic cells or evidence of human papilloma virus (HPV) infection have a discernible lipid spectrum similar to that of the malignant tissue specimen. Long T2 relaxation values found in malignant tissue specimens at 1.3 and 1.2 ppm are observed in some but not all of the biopsies which show evidence of HPV infection. The suitability of small tissue samples, such as punch biopsy specimens, for study by MR illustrates the sensitivity of this technique and its potential as an aid to histopathological discrimination between the various precursor states of cervical cancer.

Synchronous Endometrial and Cervical Tumors in a 26-Year-Old Nullipara: Diagnostic Modalities Updated

“…In our case, spectroscopy analysis of cervix and endometrium revealed high choline signals. A high level of choline suggested that the neoplasm was still metabolically active and maintained its potential to proliferation [15] . The Ki-67 proliferation index measurement for both cervical (60%) and endometrial carcinoma (55%) also supported this notion.…”

“…Choline is a cell membrane and myelin marker, which is involved in the production of acetylcholine and is observed at 3.2 ppm [13][14][15] . In most of the proliferative processes, an increased choline level can be observed.…”

“…In most of the proliferative processes, an increased choline level can be observed. The increase in choline level is considered as a sensitive index of phospholipid turnover caused by intensifi ed proliferation of neoplastic cells, indirectly pointing to its accelerated increase [13][14][15] . In our case, spectroscopy analysis of cervix and endometrium revealed high choline signals.…”

Detection of cancer in cervical tissue biopsies using mobile lipid resonances measured with diffusion-weighted 1 H magnetic resonance spectroscopy

“…MLR have been shown to be involved in tumour cell physiology, growth and metastasis (5) and cellular studies, which measure various lipid components in defined conditions, have demonstrated a correlation between an increase in MLR with suppressed proliferation (6)(7)(8), ongoing apoptosis (9)(10)(11)(12), growth arrest (7,12), and necrosis (13,14). In tissue studies, metabolic profiling of cervical biopsies (15)(16)(17) and other cancers (18,19) with magnetic resonance spectroscopy has been previously described, but measurement of MLR peaks has been confounded by signals from low molecular weight metabolites which complicate peak assignment and quantification. Several methods have been used to improve the discrimination of lipid signals from those of low molecular weight metabolites.…”

Molecular and Metabolic Consequences Following E6 Transfection in an Isogenic Ovarian Cell Line (A2780) Pair

“…Mobile lipid resonances (MLR) at 0.9 ppm and 2.0ppm are also increased in cervical cancer compared to pre-invasive (CIN) tissue [8,9]. However, because of the prevalence of HPV infection in these patients, the metabolite patterns specifically associated with the effects of viral proteins cannot be distinguished in these tissue studies.…”