Utilidad de un método que determina simultáneamente retinol y α-tocoferol en leche materna por cromatografía líquida de alta resolución

Martínez-Rojano, Hugo; Sámano, Reyna; Tolentino, Mari Cruz; Hernández, R.M.; Ramírez, Cristina; Zárate, M.L. Pizano

doi:10.1016/j.rprh.2016.11.009

Cited by 3 publications

(4 citation statements)

References 18 publications

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Section: Introductionmentioning

confidence: 99%

“…25 Antioxidant activity limits the generation of free radicals 26 and can be measured by chemical, 27 electrochemical, 28 or cellular methods. 29 Its effectiveness is evaluated with physiologically active antioxidants such as glutathione, 30 α-tocopherol, 31 ergothioneine, 32 and synthetics such as Trolox, 33 BHT, 34 and BHA. 35 Most studies that measure antioxidant activity using silver nanoparticles are based on methods such as DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2ʹazino-bis [3-ethylbenzothiazoline-6-sulfonic acid]), and FRAP (ferric reducing ability of plasma), among others.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Pilaquinga¹,

Morey²,

Fernández³

et al. 2021

IJN

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Purpose The importance of studying polyphenolic compounds as natural antioxidants has encouraged the search for new methods of analysis that are quick and simple. The synthesis of silver nanoparticles (AgNPs) using plant extracts has been presented as an alternative to determine the total polyphenolic content and its antioxidant activity. Methods In this study, aqueous leaf extract of Solanum mammosum , a species of plant endemic to South America, was used to produce AgNPs. The technique of oxygen radical absorption capacity using fluorescein (ORAC-FL) was used to measure antioxidant activity. The oxidation of the 2´,7´-dichlorodihydrofluorescein diacetate (DCFH 2 -DA) as fluorescent probe was used to measure cellular antioxidant activity (CAA). Electrochemical behavior was also examined using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Total polyphenolic content (TPH) was analyzed using the Folin-Ciocalteu method, and the major polyphenolic compound was analyzed by high performance liquid chromatography with diode array detector (HPLC/DAD). Finally, a microbial analysis was conducted using Escherichia coli and Bacillus sp. Results The average size of nanoparticles was 5.2 ± 2.3 nm measured by high-resolution transmission electron microscopy (HR-TEM). The antioxidant activity measured by ORAC-FL in the extract and nanoparticles were 3944 ± 112 and 637.5 ± 14.8 µM ET/g of sample, respectively. Cellular antioxidant activity was 14.7 ± 0.2 for the aqueous extract and 12.5 ± 0.2 for the nanoparticles. The electrochemical index (EI) was 402 μA/V for the extract and 324 μA/V for the nanoparticles. Total polyphenolic content was 826.6 ± 20.9 and 139.7 ± 20.9 mg EGA/100 g of sample. Gallic acid was the main polyphenolic compound present in the leaf extract. Microbiological analysis revealed that although leaf extract was not toxic for Escherichia coli and Bacillus sp., minor toxic activity for AgNPs was detected for both strains. Conclusion It is concluded that the aqueous extract of the leaves of S. mammosum contains nontoxic antioxidant compounds capable of producing AgNPs. The methods using AgNPs can be used as a fast analytical tool to monitor the presence of water-soluble polyphenolic compounds from plant origin. Analysis and detection of new antioxidants from plant extracts may be potentially applicable in biomedicine.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Pilaquinga¹,

Morey²,

Fernández³

et al. 2021

IJN

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“…Tocopherol extraction was carried out as explained by Shiozaki & Murakami (2016) and α-tocopherol was quantified as described by Martínez-Rojano et al (2016). Two mL of ethanol was added to grape seed oil extract, passed through 0.2 µm pore nylon filters and injected into the HPLC equipment (Series 200, Perkin Elmer, USA) with a UV detector at 290 nm, equipped with a Kromasil C18 reversed phase column of 5 µm and dimensions 4.6 x 150 mm.…”

Section: α-Tocopherolmentioning

confidence: 99%

Characterization of Black Borgoña (Vitis labrusca) and Quebranta (Vitis vinifera) grapes pomace, seeds and oil extract

et al. 2022

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Proximal composition, dietary minerals, total phenolic compounds (TPC) and antioxidant capacity (AC) were quantified in Black Borgoña (BBGP) and Quebranta grapes pomace (QGP) from Chincha and Ica (Peru). TPC and AC of grapes seeds and oil were also investigated, as quality and fatty acids of the oil. The highest amount of protein was for QGP (Chincha) (13.29 ± 0.01 g/100 g dw) and lipids in BBGP (Ica) (13.67 ± 0.01 g/100 g dw). Fe in QGP (Chincha) (61.46 ± 2.76 mg/100 g dw) and Ca in BBGP (Ica) (424.24 ± 13.35 mg/100 g dw) were higher than in the other samples. The most abundant fatty acid was linoleic with 71.70 ± 0.01% in Black Borgoña grape seed oil (BBGSO) (Chincha). Highest TPC corresponded to Quebranta grape seeds (QGS) (Ica) (14.43 ± 0.75 mg GAE/100 g dw). The highest AC by DPPH and FRAP was achieved by QGS (Chincha) (1255.39 ± 61.45 µmol TE/g dw) and QGS (Ica) (960.77 ± 56.68 µmol TE/g dw), respectively. Differences in AC by ABTS for grape seed oil were not significant (p > 0.05). In conclusion, BBGP and QGP, seeds and oil showed an enormous potential for their application as functional additives in the food industry.

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“…Tocopherol extraction was carried out as explained by Shiozaki and Murakami [18] and α-tocopherol was quantified as described by Martínez-Rojano et al [19]. Two mL of ethanol were added to the grape seed oil extract, passed through 0.2 µm pore nylon filters and injected into the HPLC equipment (Series 200, Perkin Elmer, USA) with a UV detector at 290 nm, equipped with a Kromasil C18 reversed phase column of 5 µm and dimensions of 4.6 x 150 mm.…”

Section: α-Tocopherolmentioning

confidence: 99%

Functional and chemical qualities of Vitis labrusca grape seed oil extracted by supercritical CO2

Barriga-Sánchez¹,

Rumiche²,

Sanchez-Gonzales³

et al. 2022

Rev. Colomb. Quim.

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Grape seed oil, which is extracted with highly toxic organic solvents that are harmful to human health, is produced from tons of grape pomace waste, generated during winemaking. Sometimes, this waste is used to make compost or is burnt, which causes environmental contamination. The functional qualities, antioxidant capacity (AC), α-tocopherol and total phenolic compounds content (TPC) of Black Borgoña (Vitis labrusca) grape seed oil, extracted by supercritical CO2, were evaluated. The high content of linoleic acid (w-6) and monounsaturated fatty acids contributed to the beneficial effect on the functional quality indices, which were 0.20, 0.23, 11.80 for IA, IT and H:H, respectively. In addition, a POV of 6.23 ± 0.08 milliequivalents of peroxide/kg oil and an anisidine index of 2.70 ± 0.05 indicated a good quality oil. Also, a high concentration of α-tocopherol (9.82 ± 0.02 mg/100 g oil) and a high TPC (114.14 ± 3.24 mg GAE/kg oil) were obtained. This study demonstrated that supercritical CO2 extraction is a suitable method for the delivery of a high-quality grape seed oil.

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Utilidad de un método que determina simultáneamente retinol y α-tocoferol en leche materna por cromatografía líquida de alta resolución

Cited by 3 publications

References 18 publications

Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Characterization of Black Borgoña (Vitis labrusca) and Quebranta (Vitis vinifera) grapes pomace, seeds and oil extract

Functional and chemical qualities of Vitis labrusca grape seed oil extracted by supercritical CO2

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