This study quantitatively analyzes the influence of the spectral characteristics, reflectance or transmittance, of different materials on the lighting of an interior space with natural and artificial light. For this purpose, a three-dimensional simulated classroom is used, where each of the components is assigned specific materials with an associated reflectance or transmittance. Additionally, two types of lighting are available: 6500 K daylight and light from six continuous spectrum LED luminaires. The lighting is evaluated on two planes: the work plane and the corneal plane (80 cm and 120 cm from the floor, respectively). Three versions of the same classroom were analyzed by varying the walls (white, blue, and red), each with a different neutral-colored floor. Furthermore, calculations were performed in each situation considering two different types of glazing in the windows, with 20% and 88% transmittance. The photopic and melanopic lighting analysis was carried out with the ALFA calculation program to verify the necessary requirements for adequate lighting. The results show that the white classroom is the best lit, followed by the blue and finally the red, due to the reflectance characteristics of the walls and floor although slight differences among them are found. It was found that in some cases, additional auxiliary luminaires would be required for proper lighting depending on the transmittance of the glazing. This study highlights the critical role of material selection in optimizing both photopic and melanopic lighting, with practical implications for energy efficiency and occupant well-being in educational spaces.