Utility of a green fluorone-based turn-off fluorescence probe for submicromolar determination and stability testing of two macrolides. Insights into reaction thermodynamics, quenching mechanism, and identification of the oxidative degradation products by ESI+-MS

El-Shaheny, Rania

doi:10.1016/j.microc.2019.04.037

Cited by 7 publications

(3 citation statements)

References 29 publications

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“…Therefore, eosin Y was applied to determine several pharmaceutical compounds using spectrophotometric, [ 11,26,27 ] spectrofluorimetric, [ 28–30 ] or both spectrophotometric and spectrofluorimetric [ 31–33 ] methods by forming either binary or ternary complexes. Due to binary complex formation of eosin Y with CFX, a quenching effect was produced on the native fluorescence of eosin Y at λ ex.…”

Section: Resultsmentioning

confidence: 99%

Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y

2020

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A simple, economic and validated spectrofluorimetric method was developed to assay cefixime (CFX). The technique relies on the quenching effect of CFX on the fluorescence intensity of eosin Y in the presence of acetate buffer pH 3.4 to produce an ion‐pair complex that is measured at 549 nm using an excitation wavelength of 300 nm. Reaction‐influencing factors were carefully investigated and optimized. The fluorescence quenching value was linear to the CFX concentration in the range 0.2–40 μg/ml with a correlation coefficient of 0.992. The calculated limit of detection and limit of quantification were found to be 0.00242 and 0.0080 μg ml−1, respectively. The selectivity of the method was confirmed by studying the effects of excipients and no interference was distinguished. The developed method was used to determine CFX in marketable products and in biological samples. To validate the method, directives of the International Conference on Harmonization were applied and per cent recoveries obtained ranged from 95.30 to 102.50% for pharmaceutical products and from 97.00 to 103.00% for biological fluids.

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Section: Resultsmentioning

confidence: 99%

Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y

2020

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“…ΔH° değerinin negatif olması reaksiyonun ekzotermik olduğunu göstermektedir. Ayrıca, hesaplanan ΔS° değeri negatif olarak elde edilmiştir, bu da reaksiyon kendiliğindenliğinin sıcaklığa bağlı olduğunu ve sıcaklık yeterince düşükse kendiliğinden ilerlediğini düşündürmüştür (El-Shaheny, 2019). Diğer yandan, reaksiyon hızı sabiti ile reaksiyon sıcaklığı arasındaki ilişki, Arrhenius denkleminin varyantı ile ifade edilmiştir (Eşitlik 6).…”

Section: Kinetik Ve Termodinamik çAlışmalarunclassified

Investigation of Aloe Vera Supported Silver Catalyst for Decolourization of Eosin Yellow Using Response Surface Methodology

Yılmaz

2022

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Öz: Eosin sarısı (ES) genellikle hem insan sağlığı hem de çevre için toksik bir boyadır. Bu çalışmada Aloe vera destekli gümüş (Ag/AV) katalizörü sodyum borohidrür (NaBH4) indirgeme yöntemi ile sentezlenmiştir. Sentezlenen malzeme, bir anyonik boya olan ES'nin renk gideriminde potansiyel katalizör olarak kullanılmıştır. Başlangıç ES konsantrasyonu (Co), zaman ve katalizör miktarı, NaBH4 varlığında ES renk giderimi üzerindeki etkileri araştırıldı ve proses koşulları, cevap yüzey yöntemi (response surface methodology -RSM) kullanılarak optimize edildi. Optimizasyon analizinden, 10.96 mg/L Co, 73.50 s süre ve 0.78 mg/mL katalizör miktarında %84.99'luk maksimum ES renksizleştirme verimliliği elde edildi. Ayrıca, kinetik çalışmalar, NaBH4 varlığında Ag/AV üzerinde ES'nin renksizleştirmesinin, yalancı ikinci dereceden bir kinetik modeli takip ettiğini göstermiştir. Termodinamik çalışmalar, ES renk giderme işleminin ekzotermik ve kendiliğinden olduğunu göstermiştir. Sonuç olarak hazırlanan katalizörün ES gibi organik kirleticilerin renksizleştirilmesinde verimli bir şekilde kullanılabileceği sonucuna varılabilir.

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“…Xanthene dyes and similar compounds are receiving increased interest due to their significant importance in analytical applications, affinity for binding to biomolecules, and high quantum yields [19,20]. Among these compounds, pyrosin B which is halogensubstituted fluorone base, has been employed as a dye for lasers, food coloring, and medical diagnostics.…”

Section: Introductionmentioning

confidence: 99%

An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe

Abu‐hassan,

Mahdi,

Alshehri

et al. 2024

Luminescence

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Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone‐based dye (pyrosin B). In a one‐pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle.

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Utility of a green fluorone-based turn-off fluorescence probe for submicromolar determination and stability testing of two macrolides. Insights into reaction thermodynamics, quenching mechanism, and identification of the oxidative degradation products by ESI+-MS

Cited by 7 publications

References 29 publications

Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y

Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y

Investigation of Aloe Vera Supported Silver Catalyst for Decolourization of Eosin Yellow Using Response Surface Methodology

An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe

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