Utility of glomerular morphometry in diagnosing pediatric renal disease

Kashif, A W; Verma, Nisha; Verma, Shipra; Boruah, Dibyajyoti; Sahu, Rajesh; Kalra, Suprita; Malik, Ajay

doi:10.1016/j.mjafi.2020.08.007

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…After digitalization, Bowman’s capsule and the glomerular tuft were traced and their areas were calculated using image analysis software (ImageJ). The area of Bowman’s space was determined by the difference between the area of the glomerular tuft and Bowman’s capsule [ 24 ]. Fifty glomeruli were measured in each histological sample from each animal.…”

Section: Methodsmentioning

confidence: 99%

Chronic supplementation of noni in diabetic type 1-STZ rats: effects on glycemic levels, kidney toxicity and exercise performance

de Oliveira Fernandes,

César,

Melo

et al. 2023

Diabetol Metab Syndr

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Noni is a fruit with potential medicinal use preventing elevated blood glucose levels in diabetes mellitus. Its effects have been attributed to an antioxidant property in several other diseases. However, the effects of noni-chronic supplementation on exercise performance in the presence of diabetes conditions are not known. Thirty-two male Wistar rats were used to verify the effects of chronic noni (Morinda citrifolia L) juice administration on glycemia, triglyceride levels, and its relation to physical performance. In addition, it was verified if chronic noni supplementation is safe for clinical use through kidney morphology analysis. In half of the rats, diabetes mellitus (DM) was induced with STZ. All rats were submitted to an incremental workload running test (IWT) until fatigued so that oxygen consumption and performance indexes (exercise time to fatigue and workload) could be analyzed before noni administration. Then, the control and DM groups received a placebo (saline solution) or noni juice (dilution 2:1) at a dose of 2 mL/kg once a day for 60 days. The result was four groups: control + placebo (CP), control + noni (CN), DM + placebo (DMP), and DM + noni (DMN). Our dose was based on in previous study by Nayak et al. (2011) that observed a significant reduction in glycemia with 2 ml/kg of the noni juice without any toxicity effect cited. Groups were then given a third IWT to verify the effect of the noni juice on exercise performance (exercise time to fatigue, workload, maximal oxygen consumption) and glycemia. Twenty-four hours after the third test, all animals were euthanized and blood and kidneys were removed for posterior analysis. The DM induction with STZ impaired the performance by 39%. Noni administration improved the time to fatigue and workload in DM rats beyond reducing hyperglycemia. These results could be associated with an improved energy efficiency promoted by noni ingestion, since the oxygen consumption was not different between the groups, although the exercise was longer in animals with noni ingestion. Our results provided evidence that chronic noni administration causes kidney damage since increased Bowman’s space area in the control rats, suggesting glomerular hyperfiltration at the same magnitude as the non-treated DM group.In conclusion, chronic noni ingestion promoted glycemic control and improved the performance in DM rats but caused kidney toxicity.

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Section: Methodsmentioning

confidence: 99%

Chronic supplementation of noni in diabetic type 1-STZ rats: effects on glycemic levels, kidney toxicity and exercise performance

de Oliveira Fernandes,

César,

Melo

et al. 2023

Diabetol Metab Syndr

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“…Areas of the glomerular capillary tufts were circumferenced on images captured at 200×. The ImageJ software (1.52p software 32, NIH, USA) was employed to measure the circumferenced areas using the fit ellipse parameter and presented as a percentage to the whole image 76 , 77 .…”

Section: Methodsmentioning

confidence: 99%

The suppression of MAPK/NOX/MMP signaling prompts renoprotection conferred by prenatal naproxen in weaning preeclamptic rats

Abdelhady,

Ali,

Yacout

et al. 2023

Sci Rep

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Although nonsteroidal antiinflammatory drugs (NSAIDs) are frequently used for fever and pain during pregnancy, their possible interaction with perinatal renal injury induced by preeclampsia (PE) has not been addressed. Here, studies were undertaken in the N(gamma)-nitro-l-arginine methyl ester (l-NAME) PE model to assess the influence of gestational NSAIDs on renal damage in weaning dams. PE-evoked increments and decrements in urine protein and creatinine clearance, respectively, were intensified by celecoxib and weakened by diclofenac or naproxen. Naproxen also improved renal cloudy swelling, necrosis, and reduced glomerular area evoked by PE. The concomitant rises in renal expression of markers of oxidative stress (NOX2/4), extracellular matrix metaloproteinase deposition (MMP9), and prostanoids (PGE2, PGF2α, TXA2) were all more effectively reduced by naproxen compared with celecoxib or diclofenac. Western blotting showed tripled expression of mitogen-activated protein kinases (MAPKs; p-p38, p-JNK1, p-ERK1, p-ERK2) in PE kidneys that was overturned by all NSAIDs, with naproxen producing the largest drop in p-ERK2 expression. The PE-provoked elevation in renal expression of autophagic marker LC3 was reduced by naproxen and diclofenac, but not celecoxib. The data suggests superior effect for naproxen over other NSAIDs in rectifying preeclamptic renal injury and predisposing inflammatory, oxidative, autophagic, and fibrotic signals.

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“…The images at 40× magnification from the H&E stained slides were used to assess corpuscular and glomerular tuft areas under 40× magnification, and glomerular density under 10× magnification. Glomerular urinary space area was computed using the formula (Kashif et al 2020): Glomerular urinary space area (µm 2 ) = renal corpuscular area (µm 2 ) -glomerular tuft area (µm 2 ). Glomerular density was computed using the following formula (Nigro et al 2018): Glomerular density (N/µm 2 ) = number of renal corpuscle per section (N) ÷ total area of the section (µm 2 ).…”

Section: Histological Assessment Of the Kidneymentioning

confidence: 99%

Neonatal administration of zingerone prevents the subsequent development of high dietary fructose-induced early features of nephropathy in rats

Muhammad¹,

Lembede²,

Erlwanger³

2023

gpb

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Excessive consumption of fructose-rich diets in early life stages increases the risk for developing nephropathy in adulthood. We investigated the potential preventive effects of neonatally administered zingerone on the development of dietary fructose-induced nephropathy. Four-day-old suckling male and female rat pups were orally gavaged (10 ml/kg) with: distilled water (Con group), 20% fructose solution (Fru group), 20% fructose solution + 40 mg/kg zingerone in distilled water (ZFru group), or 40 mg/kg of zingerone (Zgr group) for 14 days. Thereafter, Con and Zgr groups continued on plain drinking water while Fru and ZFru groups drank 20% fructose solution ad libitum for 10 weeks. The Fru group had significantly increased plasma concentration of the renal injury marker kidney injury molecule one (KIM-1) and decreased glomerular urinary space area compared to the controls in both sexes (p < 0.05). These alterations were prevented by neonatally administered zingerone. Zingerone administration neonatally is a potential prophylaxis for longterm high-fructose diet-induced nephropathy.

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Utility of glomerular morphometry in diagnosing pediatric renal disease

Cited by 6 publications

References 17 publications

Chronic supplementation of noni in diabetic type 1-STZ rats: effects on glycemic levels, kidney toxicity and exercise performance

Chronic supplementation of noni in diabetic type 1-STZ rats: effects on glycemic levels, kidney toxicity and exercise performance

The suppression of MAPK/NOX/MMP signaling prompts renoprotection conferred by prenatal naproxen in weaning preeclamptic rats

Neonatal administration of zingerone prevents the subsequent development of high dietary fructose-induced early features of nephropathy in rats

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