Utility of immunological tests for bird-related hypersensitivity pneumonitis

Suhara, Kozo; Miyazaki, Yasunari; Okamoto, Tsukasa; Yasui, Makito; Tsuchiya, Kimitake; Inase, Naohiko

doi:10.1016/j.resinv.2014.08.001

Cited by 37 publications

(32 citation statements)

References 36 publications

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“…However, because these cells are exceedingly rare in the blood, PBMCs proliferation assay is specific but not sufficiently sensitive for a diagnosis. As previously reported, patients with BRHP showed increasing proliferation not more than 50-60% with PBMCs proliferation assay using crude pigeon plasma [32, 33]. …”

Section: Discussionsupporting

confidence: 54%

Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping

et al. 2017

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BackgroundAvian antigen is a common cause of hypersensitivity pneumonitis (HP). Inhalation challenge with pigeon serum and pigeon dropping extract (PDE) elicits a hypersensitivity reaction in patients with bird-related hypersensitivity pneumonitis (BRHP), but the antigenic components in these materials have yet to be fully elucidated.MethodPigeon serum, pigeon intestine homogenates, and PDE were immunoblotted with serum samples from 8 patients with BRHP, 2 patients with summer-type HP, 2 patients with humidifier lung, and 3 healthy volunteers. Among the protein spots found in both pigeon serum and PDE, those that reacted with sera from BRHP patients were identified by mass spectrometry. Immunoassays using recombinant protein were performed to confirm the antigenicity of the identified protein. Cytokine production by peripheral blood mononuclear cells (PBMCs) stimulated with recombinant protein was also assessed.ResultsImmunoglobulin lambda-like polypeptide-1 (IGLL-1) was identified from all spots on 2-DE immunoblots of both pigeon serum and PDE. The BRHP patients exhibited higher levels of serum IgG antibody against the recombinant IGLL-1 (rIGLL-1) compared to the control subjects, as well as a stronger PBMCs proliferative response to rIGLL-1. Cytokine production by PBMCs from BRHP patients after rIGLL-1 exposure indicated that the protein could induce Th1 prone immune responses: an increase in TNF-α and an absence of elevated IL-10 production.ConclusionsPigeon IGLL-1 was identified as the BRHP antigen present in both pigeon serum and PDE.Electronic supplementary materialThe online version of this article (doi:10.1186/s12931-017-0555-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 54%

Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping

et al. 2017

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“…Although the pathogenesis of PBL is known to be closely related to long-term exposure to exogenous antigens and immune reactions are known to mediate lung injury, the cellular immunological mechanism of HP remains unclear [15]. In the early stages of the immune response, proteins in pigeon dropping products, such as feathers and dander, are recognized and phagocyted by macrophages in the lung, forming an immunocomplex with IgG and activating …”

Section: Discussionmentioning

confidence: 99%

Associations of Pulmonary Fibrosis with Peripheral Blood Th1/Th2 Cell Imbalance and EBF3 Gene Methylation in Uygur Pigeon Breeder’s Lung Patients

Wu¹,

Luo²,

Pang³

et al. 2018

Cell Physiol Biochem

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Background/Aims Pigeon breeder’s lung (PBL) results from Th1/Th2 cell imbalance. B cells inhibit the immune activity of Th1, and EBF3 is a key B cell factor. This study explored the relationship between EBF3 and Th1/Th2 imbalance in chronic PBL cases complicated with pulmonary fibrosis (PF). Methods Twenty Uygur PBL+PF patients, 20 pigeon breeders without PBL or PF, and 20 healthy individuals without pigeon breeding history constituted the patient I, negative control, and normal control groups, respectively. Peripheral blood specimens and case backgrounds were collected between June 2016 and March 2017. EBF3 gene methylation was analyzed by matrix assisted laser desorption ionization-time of flight mass spectrometry. To compare different mechanisms of PF progression in PBL, samples from 20 Uygur PBL patients without PF (at acute and sub-acute stages) were collected between October 2017 and February 2018, constituting the patient II group. EBF3 mRNA expression was evaluated by real-time polymerase chain reaction. IFN-γ, IL-4 and IL-10 expression and Th1/Th2 imbalance in PBL were evaluated by enzyme-linked immunosorbent assay and flow cytometry. Results CpG-2 and general methylation rates in the patient I group were lower than those in the control groups (P˂0.017). The level of EBF3 mRNA expression in the patient I group was significantly higher than that in any other group. Compared with the control groups, the patient I group showed a significantly higher level of IL-4, whereas the patient II group showed a significantly lower level. IL-10 was also expressed more highly in the patient I group than in any other group (P< 0.01). Flow cytometry showed INF-γ dominance (Th1 cytokine) in PBL at the acute/sub-acute stage and IL-4 dominance (Th2 cytokine) at the chronic stage after PF occurred. The general methylation rate was negatively correlated with the mRNA level, with the latter being positively correlated with the IL-10 level and number of pigeons bred in the past 3 months. IL-4 expression was negatively correlated with INF-γ but positively correlated with PF area and duration of pigeon breeding history. Conclusions After PF occurs in chronic PBL, the inflammation type changes from Th1 dominance to Th2 dominance. During PBL development, IL-10 increases before IL-4 does, which may be associated with EBF3 hypomethylation and the involvement of B lymphocytes.

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“…In addition, inhibition testing with the patient serum can be used as a detection tool for identifying the offending antigen source . Lymphocyte proliferation tests with the suspected antigen cannot be used in routine practice as the method has not been standardized, but in selected cases, for example using BAL cells from patients with acute bird‐related HP, this test may be a useful diagnostic tool and highlight the important role of cellular immunity in the onset of bird‐related HP .…”

Section: Diagnosismentioning

confidence: 99%

Occupational hypersensitivity pneumonitis: an EAACI position paper

Quirce

Vandenplas

Campo

et al. 2016

Allergy

154

109

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The aim of this document was to provide a critical review of the current knowledge on hypersensitivity pneumonitis caused by the occupational environment and to propose practical guidance for the diagnosis and management of this condition. Occupational hypersensitivity pneumonitis (OHP) is an immunologic lung disease resulting from lymphocytic and frequently granulomatous inflammation of the peripheral airways, alveoli, and surrounding interstitial tissue which develops as the result of a non-IgE-mediated allergic reaction to a variety of organic materials or low molecular weight agents that are present in the workplace. The offending agents can be classified into six broad categories that include bacteria, fungi, animal proteins, plant proteins, low molecular weight chemicals, and metals. The diagnosis of OHP requires a multidisciplinary approach and relies on a combination of diagnostic tests to ascertain the work relatedness of the disease. Both the clinical and the occupational history are keys to the diagnosis and often will lead to the initial suspicion. Diagnostic criteria adapted to OHP are proposed. The cornerstone of treatment is early removal from exposure to the eliciting antigen, although the disease may show an adverse outcome even after avoidance of exposure to the causal agent.

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Utility of immunological tests for bird-related hypersensitivity pneumonitis

Cited by 37 publications

References 36 publications

Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping

Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping

Associations of Pulmonary Fibrosis with Peripheral Blood Th1/Th2 Cell Imbalance and EBF3 Gene Methylation in Uygur Pigeon Breeder’s Lung Patients

Occupational hypersensitivity pneumonitis: an EAACI position paper

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