Utilization of a complex arrangement approach for spectroscopic examination with Eosin Y of various cephalosporins in their pure or pharmaceutical dosage forms, and in human plasma

Salem, Hesham; Omar, Mahmoud A.; Mazen, Dina Z; El-Deen, Deena A.M. Nour

doi:10.1002/bio.4057

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…[23] Complexes formed between the ion-pairing agent eosin Y and numerous drugs for spectrophotometric or spectrofluorimetric investigation have been published in the literature. [24][25][26][27][28][29] Because many extraction processes utilize halogenated solvents, which are hazardous to humans and the surrounding environment, these methods have several disadvantages, including reduced sensitivity and decreased simplicity. In this work, eosin is used for the determination of GTF and the formation of the binary complex without further extraction procedures for the resulting complex.…”

Section: Introductionmentioning

confidence: 99%

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Gammal

El‐Wasseef

El‐Ashry

et al. 2022

J Chinese Chemical Soc

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Multiple sensitive eco-friendly analytical techniques were applied for the assessment of two important fourth-generation fluoroquinolones namely, gemifloxacin (GFX), and gatifloxacin (GTF). GFX, which is utilized as an essential adjunct therapy for the management of pneumonia in SARS-CoV-2 was determined by green indicating micellar liquid chromatographic in authentic powder and dosage forms. The separation was performed by using a reversed-phase C 18 column; the micellar mobile phase comprised of 12.5% npropanol, 0.15 M sodium lauryl sulfate, and 0.3% triethylamine in 0.02 M Ophosphoric acid adjusted to pH 3.0 and pumped at 1 ml/min flow rate and detected at 266 nm by UV detection. The method was linear over the range of 4.0-90 μg.ml À1 with a recovery percentage of 99.81 ± 0.69. The acidic degradation behavior of GFX along with the kinetic investigation was evaluated as recommended by ICH-stress conditions. The separation of the degradation product was performed in good elution time (less than 8 min), and the suggested technique has a good reproducibility (R.S.D. less than 1.0%) and provides an excellent resolution (R s = 4.5) between GFX and its degradation product. Concerning the second drug, GTF, simple, extremely sensitive, and accurate spectrofluorimetric and spectrophotometric techniques were adopted for the determination of the drug in its pharmaceutical preparations. The suggested approaches utilize the concept of complex formation with eosin Y in an aqueous buffered solution. Regarding the spectrophotometric method (method I), the binary complex has a maximum absorption at 544 nm, while for spectrofluorimetric determination (method II) the formed complex was recorded at λ em 491 nm after excitation at 286 nm. For both approaches, variables affecting the formation of the binary complexes were extensively investigated and optimized. The suggested techniques were shown to be rectilinear across the concentration range of 0.5-8 and 0.05-1 μg.ml À1 , respectively, with recovery percentages of 100.34 ± 0.85 and 99.90 ± 0.81 for method (I) and method (II), respectively. In compliance with ICH guidelines, the analytical performance of

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Section: Introductionmentioning

confidence: 99%

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Gammal

El‐Wasseef

El‐Ashry

et al. 2022

J Chinese Chemical Soc

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Analysis of Two Oncological Drugs Futibatinib and Capivasertib via Ion‐Pairing With Eosin Y as a Spectrofluorimetric and Spectrophotometric Probe

Salem,

Emad,

El‐Araby

et al. 2024

Luminescence

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Using spectroscopy, two quick and sensitive analytical methods based on eosin Y ion pairing were developed and assessed in order to determine capivasertib and futibatinib with high selectivity and sensitivity. The quenching impact of futibatinib or capivasertib on the eosin Y's fluorescence at a pH 3.8 and 3.3 for futibatinib and capivasertib, respectively, in 0.1‐M acetate buffer solution was observed using two spectrofluorometric techniques. These techniques are regarded as the original spectrofluorometric techniques for the assay of futibatinib and capivasertib. For futibatinib and capivasertib, the quenching effect on fluorescence was ranged from 100 to 1000 and 150 to 1500 ng mL−1, respectively. The absorbance of the generated ion‐pair was measured using two different spectrophotometric methods at 550 nm in aqueous buffered solutions with pH values of 3.8 and 3.3 for futibatinib and capivasertib, respectively. In the concentration range of 1.0–10.0 and 2.0–10.0 μg mL−1, Beer's law was followed. The four approaches were applied to the analysis of dosage forms with a high percent recovery successfully, and they were assessed in compliance with ICH guidelines.

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Simultaneous Determination of Ceftazidime in Three Different Pharmaceutical Preparations Combined with Either Tazobactam, Tobramycin or Sulbactam by HPTLC-Spectrodensitometric Method

Salem

Omar

Mazen

et al. 2023

Journal of Chromatographic Science

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A new, simple hight performance thin layer chromatography (HPTLC)-Spectrodensitometric strategy was created and approved for the synchronous estimation of four antibacterial specialists: ceftazidime (CEF), tazobactam (TAZ), tobramycin (TOB) and sulbactam (SUL). The four compounds were separated on TLC aluminum plates covered with silica gel 60 F254, using chloroform–acetonitrile–methanol–ammonia (4:1:0.5:0.15, v/v/v/v) as a mobile phase at 254 nm. Linear correlation was obeyed over the concentration ranges of 12.0–72.0, 2.0–12.0, 3.0–18.0 and 10.0–50.0 μg mL−1 for CEF, TAZ, TOB and SUL, respectively. The proposed approach is efficient, repeatable and convenient as a flexible method for the quality control of diverse combinations of these pharmaceuticals in various pharmaceutical preparations, with high percent recoveries that are highly consistent with labeled data. When the findings of the proposed technique were compared to those of the comparison methods, there were no critical contrasts in terms of precision and accuracy.

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Utilization of a complex arrangement approach for spectroscopic examination with Eosin Y of various cephalosporins in their pure or pharmaceutical dosage forms, and in human plasma

Cited by 3 publications

References 38 publications

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Analysis of Two Oncological Drugs Futibatinib and Capivasertib via Ion‐Pairing With Eosin Y as a Spectrofluorimetric and Spectrophotometric Probe

Simultaneous Determination of Ceftazidime in Three Different Pharmaceutical Preparations Combined with Either Tazobactam, Tobramycin or Sulbactam by HPTLC-Spectrodensitometric Method

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