Utilization of coffee pulp as a substrate for pectinase production by Aspergillus sp. VTMS through solid state fermentation

Hidayah, Atim Ainul; Azizah,; Winarsa, Rudju; Muzakhar, Kahar

doi:10.1063/5.0030474

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…Optimum incubation time was known by counting the reducing sugar level produced by Aspergillus sp. VTM5 [5]. Optimization of pectinase production by Aspergillus sp.…”

Section: Pectinase Production By Aspergillus Sp Vtm5mentioning

confidence: 99%

“…This fungus has been known to hydrolyze POEFB and produce a reducing sugar of 5.57 µg/ml [4]. Other than that, this fungus was also known to be able to produce pectinase using coffee pulp waste as a substrate through solid-state fermentation [5].…”

Section: Introductionmentioning

confidence: 99%

“…Aspergillus sp. VTM5 based on Hidayah et al [5] can produce pectinase with a reducing sugar concentration of 85.9 µg/ml after 72 h incubation using coffee pulp as a substrate through solid-state fermentation. Crude pectinase has a stable pH range of 3-5, while the optimum pH is 4.5.…”

Section: Introductionmentioning

confidence: 99%

“…Crude pectinase has a stable pH range of 3-5, while the optimum pH is 4.5. However, in Hidayah et al [5] research, large-scale production process and purification of pectinase have not been carried on. Therefore, this research aims to produce and purify pectinase from Aspergillus sp.…”

Section: Introductionmentioning

confidence: 99%

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Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

Azizah¹,

Hidayah²,

Amelia³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Hydrolytic Pectinase is an important enzyme and is widely used in several industries. In the previous investigation, this enzyme could be produced by Aspergillus sp. VTM5 under solid-state fermentation and the coffee pulp waste substrates were used. The specific activity of about 0.4 U/ml of pectinase was detected when 40gr coffee pulp water-saturated inoculated with 1.2x10 7 spores of Aspergillus sp. VTM5 was incubated at 30 °C for 168 h, and without any nutrients added. Against 0.5% alkaline extract of pomelo pulp substrate in 20 Mm acetate buffer pH 5, pectinase activity increases up to 1.03 U/ml, and the purity 3.5 times, after dialyzed using 12-14 kDa, pores cellulose membrane tube higher than previously step. Open column purification using Fractogel EMD SO3-(S) cation exchange chromatography, pectinase was eluted at 0.45 M NaCl. The pectinase activity significantly increased to 0.295 U/ml with 12.37-fold and 17.04% yield. Based on these results, it can be proved that coffee pulp waste is suitable as a substrate for pectinase production by Aspergillus sp. VTM5. Further research to analyze the properties and characterize the stability of pectinase against temperature, pH, the effect of heavy metals, and molecular analysis is needed.

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“…Optimum incubation time was known by counting the reducing sugar level produced by Aspergillus sp. VTM5 [5]. Optimization of pectinase production by Aspergillus sp.…”

Section: Pectinase Production By Aspergillus Sp Vtm5mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

Azizah¹,

Hidayah²,

Amelia³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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“…The addition of 4ml distilled water to attain the nal volume and OD was measured at 570nm. One unit of enzyme activity corresponds to the micromoles of glucose as reducing sugar molecules produced under the reaction condition per minute (Hidayah et al, 2020) Thermal Deactivation of Crude Enzyme Extract Thermal studies were experimented by incubating the crude enzyme extract at speci c temperature in the absence of the substrate. The standard amount of enzyme extracts were taken in test tube and placed in waterbath.…”

Section: Solid State Fermentationmentioning

confidence: 99%

Kinetic Analysis of Crude Enzyme Extract Produced Via Solid State Fermentation of Banana Pseudo Stem Waste

Subash

Perumalsamy²

2021

Preprint

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Banana Pseudo stem waste after each harvest contributes about 70–80 Milli Tons Per hector. The banana pseudo stem will be thrown as waste biomass after each harvest as it is unstable for the upcoming harvest. The biggest challenge in banana cultivation is the utilization of biomass of banana pseusostem waste into valuable products. In this study, Xylano-pectinase enzyme extract was produced from banana pseudo stem waste under solid-state fermentation by Enterobacter cloacae PMC04. The highest pectinase and xylanase activities obtained using banana pseudo stem as carbon source were 124.62 U/ml and 173.81 U/ml respectively. Thermodynamics stated that range 40-50oC were considered to be the optimal temperature for xylano-pectinase enzyme production and subsequent degumming of banana fibers. The crude enzyme extract were then used in the degumming of banana fibers for textile application. Textile processing of banana fiber necessitates the removal of hemicellulose substance which can be achieved by crude xylano-pectinase enzyme. It was found that crude xylano-pectinase was efficient in the removal of hemicellulose substance from the fibers. Results obtained from this study demonstrate that the proposed bioprocess could be successfully applied for the degumming of banana fibers sustainably.

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Agro‐Wastes for Cost Effective Production of Industrially Important Microbial Enzymes

Dwivedi

Tanveer

Yadav

et al. 2022

Microbial Biotechnology

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Utilization of coffee pulp as a substrate for pectinase production by Aspergillus sp. VTMS through solid state fermentation

Cited by 9 publications

References 16 publications

Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

Kinetic Analysis of Crude Enzyme Extract Produced Via Solid State Fermentation of Banana Pseudo Stem Waste

Agro‐Wastes for Cost Effective Production of Industrially Important Microbial Enzymes

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