Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29

Schmid, Maximilian T.; Song, Hyunjeong; Raschbauer, Michaela; Emerstorfer, Florian; Omann, Markus; Stelzer, Franz; Neureiter, Markus

doi:10.1016/j.procbio.2019.08.001

Cited by 32 publications

(31 citation statements)

References 23 publications

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“…Nevertheless, pyrogenic lipopolysaccharides present in the outer membranes of Gram-negative bacteria are co-isolated along with PHA and represent serious contamination of the material, which complicates the utilization of PHA in numerous fields such as medicine, cosmetics or food packaging [ 45 ]. Moreover, the Gram-positive Bacillus and related species can be used for PHA synthesis [ 46 ] which overcome the problems with PHA materials contamination by pyrogens. Nevertheless, bacilli are spore-forming bacteria which bring numerous complications to the process since sudden sporulation stops the desirable metabolic activity of the culture and is associated with the mobilization of PHA storage which reduces product titers and yields [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

et al. 2021

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Actinobacteria belonging to the genus Rubrobacter are known for their multi-extremophilic growth conditions—they are highly radiation-resistant, halotolerant, thermotolerant or even thermophilic. This work demonstrates that the members of the genus are capable of accumulating polyhydroxyalkanoates (PHA) since PHA-related genes are widely distributed among Rubrobacter spp. whose complete genome sequences are available in public databases. Interestingly, all Rubrobacter strains possess both class I and class III synthases (PhaC). We have experimentally investigated the PHA accumulation in two thermophilic species, R. xylanophilus and R. spartanus. The PHA content in both strains reached up to 50% of the cell dry mass, both bacteria were able to accumulate PHA consisting of 3-hydroxybutyrate and 3-hydroxyvalerate monomeric units, none other monomers were incorporated into the polymer chain. The capability of PHA accumulation likely contributes to the multi-extremophilic characteristics since it is known that PHA substantially enhances the stress robustness of bacteria. Hence, PHA can be considered as extremolytes enabling adaptation to extreme conditions. Furthermore, due to the high PHA content in biomass, a wide range of utilizable substrates, Gram-stain positivity, and thermophilic features, the Rubrobacter species, in particular Rubrobacter xylanophilus, could be also interesting candidates for industrial production of PHA within the concept of Next-Generation Industrial Biotechnology.

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Section: Discussionmentioning

confidence: 99%

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

et al. 2021

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“…Sugar beet (Schmid et al, 2019) Desugarized sugar beet (Schmid et al, 2019) Dry matter % 73. production and its utilization leads to 9-11% replacement of the total energy demand provided by bagasse combustion (data were only available from bioethanol plants based on sugar cane) (Nakashima and de Oliveira Junior, 2020). An alternative utilization of biogas was described by Leme and Seabra (2017).…”

Section: Unit Sugar Canementioning

confidence: 99%

“…The residues from sugar beet processing are sugar beet pulp (SBP) and molasses which are obtained in large amounts (Suhartini et al, 2018;Schmid et al, 2019). Per ton of fresh sugar beet, 140 kg sugar, 300-350 kg SBP and 35 to 50 kg molasses arise (Stoyanova et al, 2014;Zieminski and Kowalska-Wentel, 2017;Schmid et al, 2019). The composition of molasses from sugar cane is presented in Table 7.…”

Section: Sugar Beetmentioning

confidence: 99%

“…The production of sugar from sugar cane and sugar beet takes place in several steps and during both processes, molasses is obtained in large amounts with a high residual sugar content. (Ergüder et al, 2001) SBP (Suhartini et al, 2018) Bagasse (Janke et al, 2016) Every ton of sugar produced requires a raw amount of 7 t of sugar beet with 0.25-0.35 t of molasses as a by-product (Schmid et al, 2019). The main utilization of molasses lies in the pharmaceutical and chemical industry, as animal feed or fermentation substrate for the production of baker's yeast, rum, or bioethanol (Eggleston and Lima, 2015;Matissek, 2016).…”

Section: Utilization Of Molasses From Sugar Productionmentioning

confidence: 99%

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Anaerobic Digestion of Pretreated Industrial Residues and Their Energetic Process Integration

Bochmann

Pesta

Rachbauer

et al. 2020

Front. Bioeng. Biotechnol.

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The food and beverage industry offers a wide range of organic feedstocks for use in biogas production by means of anaerobic digestion (AD). Microorganisms convert organic compounds-solid, pasty, or liquid ones-within four steps to biogas mainly consisting of CH 4 and CO 2. Therefore, various conversion technologies are available with several examples worldwide to show for the successful implementation of biogas technologies on site. The food and beverage industry offer a huge potential for biogas technologies due to the sheer amount of process residues and their concurrent requirement for heat and power. The following study analyzes specific industries with respect to their implementation potential based on arising waste and heat and power demand. Due to their chemical composition, several feedstocks are resistant against microbiological degradation to a great extent. A combination of physical-, chemical-, and microbiological pretreatment are used to increase the biological availability of the feedstock. The following examples will discuss how to best implement AD technology in industrial processes. The brewery industry, dairy production, slaughterhouses, and sugar industry will serve as examples.

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“…In this study desugarized sugar beet molasses (DSBM) was used because it is available in large quantities and it can be directly used as the sole fermentation substrate. 22 After the fermentation the biomass is harvested and the PHA must be extracted, which could be responsible for up to 50% of the total production costs. 23 Chlorinated solvents like chloroform, dichloromethane and 1,2-dichloroethane are commonly used for extraction.…”

Section: Introductionmentioning

confidence: 99%

Pilot scale production and evaluation of mechanical and thermal properties of P(3HB) from Bacillus megaterium cultivated on desugarized sugar beet molasses

Schmid

Sykacek

O’Connor³

et al. 2021

J of Applied Polymer Sci

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Production of poly[(R)-3-hydroxyalkanoate] (PHA) biopolyesters must become more cost efficient in order to be competitive with conventional plastics as well as other bioplastics. This study introduces a simple and reproducible largescale production process of poly(3-hydroxybutyrate) P(3HB) with Bacillus megaterium based on desugarized sugar beet molasses and compares its material properties with commercially available types of PHA. A repeated batch cultivation system was evaluated in 1.2 L bioreactors and could be maintained for at least six cycles without a decrease in process stability, yielding a concentration of 20.4 g/L cell dry mass and 12 g/L P(3HB) per average process cycle. Feasibility of P(3HB) production was demonstrated at 500 L pilot scale to provide sufficient amounts of product for the determination of mechanical and thermal polymer properties. A volumetric productivity of 0.2 g L À1 h À1 was achieved.The resulting polymer exhibited a high tensile modulus of 3.73 GPa and tensile strength of 38.5 MPa. Despite a lower ductility and Vicat softening temperature, the properties of the recovered P(3HB) are comparable to commercial grade P(HB)HV polymers.

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Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29

Cited by 32 publications

References 23 publications

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

Anaerobic Digestion of Pretreated Industrial Residues and Their Energetic Process Integration

Pilot scale production and evaluation of mechanical and thermal properties of P(3HB) from Bacillus megaterium cultivated on desugarized sugar beet molasses

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