Utilization of Eosin Dye as an Ion Pairing Agent for Determination of Pharmaceuticals: A Brief Review

Abstract

Globally, dyes are widely used in the pharmaceutical, food, textile, cosmetics, plastics, leather, paint, ink and paper industries. Eosin is an acidic orange-pink dye and has very strong staining properties. Haematoxylin and eosin Y (H and E) combination is the most common staining and primary diagnostic technique in histo-pathological laboratories. This review mainly discussed the utility of eosin dye in quality control laboratories as an ion pairing agent for drug analysis. Eosin Y is one the most common ion pairing agent and its mono and di anionic forms are capable of interacting with many drug molecules to form colored/fluorescent binary or ternary complexes that can be analyzed with or without extraction by spectrofluorimetry and/or spectrophotometry. Quenching fluorescence and advantages of spectrofluorimetry over spectrophotometry were also discussed. Fluorescence detection greatly enhances the sensitivity and providing a sensitive and relatively inexpensive instrumental method of analysis using eosin Y for various important drugs in pure, commercial dosage forms and biological fluids.

Inhibition Effect of Eosin Y on Carbonic Anhydrase (CA) I and II Isoenzymes Purified from Human Erythrocytes

“…In conclusion, Eosin Y, the most commonly used xanthan dye in histological and drug analysis studies (Rahman, 2017), showed inhibition effect on hCA I and hCA II isoenzymes. Carbonic anhydrase inhibitors are drug candidates for the treatment of many diseases, from glaucoma to neuropathic pain (Supuran, 2018).…”

“…In addition, Eosin Y is also one of the most common ion-coupling agents, and its mono and di-anionic forms can interact with many drug molecules. Therefore, it belongs to the xanthan dye group, which is widely used in the analysis of many drugs (Waheed et al, 2000;Rahman, 2017). In this study, in order to investigate the inhibitory effects of eosin Y on hCA I and hCA II isoenzymes, firstly, CA I and CA II isoenzymes were purified from human erythrocytes and the purification table was prepared with the data obtained (Table 1).…”

“…Combination of Eosin Y with hematoxylin is the most common staining and primary diagnostic technique used in histopathological studies. Eosin Y is also one of the most common ion-coupling agents, and its mono and di-anionic forms can interact with many drug molecules (Waheed et al, 2000;Rahman, 2017). The aim of this study was to investigate the inhibitory effect of Eosin Y on CA I and CA II isoenzymes of human erythrocytes.…”

A Novel Spectrophotometric Determination of Methyldopa Through Ternary Complexation Procedure Using Fe(iii), Mn(ii), and Co(ii) With 2-Aminopyridine

“…The ternary complex formation has been considered to be of an important role in the field of spectrophotometric analysis [13,14]; they are divided into two types, ion-association and a mixed-ligand complex. Different pharmaceutical compounds have been analyzed using ion-pair complexes [15,16]. Meanwhile, the formation of ternary complexes starts to get much attention [17,18].…”

Photophysical and antibacterial activity of light-activated quaternary eosin Y

“…They belong to the group of xanthene fluorescent dyes with a wide range of photophysical and biological applications, due to their low toxicity in vivo, and high water solubility [3]. The utility of eosin derivatives is associated to their good spectral characteristics and the possibility to interact with different type of biomolecules [4][5][6][7][8]. Photophysical properties of eosin in solution strongly depend on the solvents polarity and possibility of hydrogen bond formations.…”